Antibiotics,
Год журнала:
2023,
Номер
12(5), С. 794 - 794
Опубликована: Апрель 22, 2023
Diabetic
foot
ulcers
(DFU)
are
a
major
complication
of
diabetes
mellitus
and
public
health
concern
worldwide.
The
ability
P.
aeruginosa
to
form
biofilms
is
key
factor
responsible
for
the
chronicity
diabetic
infections
(DFIs)
frequently
associated
with
presence
persister
cells.
These
subpopulation
phenotypic
variants
highly
tolerant
antibiotics
which
new
therapeutic
alternatives
urgently
needed,
such
as
those
based
on
antimicrobial
peptides.
This
study
aimed
evaluate
inhibitory
effect
nisin
Z
DFI
persisters.
To
induce
development
state
in
both
planktonic
suspensions
biofilms,
isolates
were
exposed
carbonyl
cyanide
m-chlorophenylhydrazone
(CCCP)
ciprofloxacin,
respectively.
After
RNA
extraction
from
CCCP-induced
persisters,
transcriptome
analysis
was
performed
differential
gene
expression
between
control,
cells
Z.
Nisin
presented
high
against
but
unable
eradicate
them
when
present
established
biofilms.
Transcriptome
revealed
that
persistence
downregulation
genes
related
metabolic
processes,
cell
wall
synthesis,
dysregulation
stress
response
biofilm
formation.
treatment,
some
transcriptomic
changes
induced
by
reversed.
In
conclusion,
could
be
considered
potential
complementary
therapy
treating
DFI,
it
should
applied
an
early
treatment
or
after
wound
debridement.
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(15), С. 18360 - 18385
Опубликована: Апрель 4, 2024
Bacterial
keratitis
(BK)
causes
visual
morbidity/blindness
if
not
treated
effectively.
Here,
ciprofloxacin
(CIP)-loaded
nanoparticles
(NPs)
using
glycol
chitosan
(GC)
and
poly(lactic
acid)
(PLA)
conjugate
at
three
different
ratios
(CIP@GC(PLA)
NPs
(1:1,5,15))
were
fabricated.
CIP@GC(PLA)
(1:1)
more
effective
than
other
tested
ratios,
indicating
the
importance
of
optimal
hydrophobic/hydrophilic
balance
for
corneal
penetration
preventing
bacterial
invasion.
The
realized
highest
association
with
human
epithelial
cells,
which
nonirritant
to
hen's
egg-chorioallantoic
membrane
test
(HET-CAM
test)
demonstrated
significant
antibacterial
response
in
vitro
minimum
inhibitory,
bactericidal,
live-dead
zone
inhibition,
biofilm
inhibition
assays
against
keratitis-inducing
pathogen
Pseudomonas
aeruginosa.
antiquorum
sensing
activity
GC
has
been
explored
first
time.
disrupted
quorum
by
inhibiting
production
virulence
factors,
including
acyl
homoserine
lactones,
pyocyanin,
motility,
caused
downregulation
associated
genes.
In
vivo
studies,
displayed
ocular
retention
(∼6
h)
decreased
opacity
load
Overall,
NP
is
a
biofilm-disrupting
treatment
regimen
clinical
translation
potential
BK.
Biofilm,
Год журнала:
2024,
Номер
8, С. 100208 - 100208
Опубликована: Июнь 20, 2024
Pseudomonas
aeruginosa
is
recognized
globally
as
an
opportunistic
pathogen
of
considerable
concern
due
to
its
high
virulence
and
pathogenicity,
especially
in
immunocompromised
individuals.
While
research
has
identified
several
endogenous
quorum
sensing
(QS)
signaling
molecules
that
enhance
the
pathogenicity
P.
aeruginosa,
investigations
on
exogenous
QS
or
modulating
factors
remain
limited.
This
study
found
dopamine
serves
molecule
factor
PAO1,
enhancing
production
biofilms.
Compared
control
group,
treatment
with
40
μM
resulted
a
33.1%
increase
biofilm
formation,
68.1%
swimming
mobility,
63.1%
swarming
147.2%
3-oxo-C12-HSL,
50.5%,
28.5%,
27.0%,
33.2%
increases
alginate,
rhamnolipids,
protease,
pyocyanin,
respectively.
further
explored
mechanism
regulating
formation
PAO1
through
transcriptome
metabolome.
Transcriptomic
analysis
showed
promoted
expression
genes
psl,
alg,
lasA,
rhlABC,
rml,
phz
PAO1.
Metabolomic
revealed
changes
concentrations
tryptophan,
pyruvate,
ethanolamine,
glycine,
3-hydroxybutyric
acid,
alizarin.
Furthermore,
KEGG
enrichment
altered
metabolites
indicated
enhanced
phenylalanine,
tyrosine,
tryptophan
The
results
this
will
contribute
development
novel
advance
our
understanding
interactions
between
host
environment.
Frontiers in Microbiology,
Год журнала:
2023,
Номер
14
Опубликована: Ноя. 9, 2023
Bacterial
biofilm
is
an
emerging
form
of
life
that
involves
cell
populations
living
embedded
in
a
self-produced
matrix
extracellular
polymeric
substances
(EPS).
Currently,
little
known
about
the
molecular
mechanisms
Bifidobacterium
formation.
We
used
fermentation
system
to
preparation
biofilms
on
wheat
fibers,
and
multi-omics
analysis
both
B.
pseudocatenulatum
planktonic
cells
were
performed
identify
genes
metabolites
involved
The
average
diameter
fibers
was
around
50
μm,
while
particle
culture
over
260
μm
at
22
h
with
78.96%
formation
rate
(BR),
field
emission
scanning
electron
microscopy
(FESEM)
results
showed
surface
secreted
EPS.
Transcriptomic
indicated
associated
stress
response
(
groS
,
mntH
nth
pdtaR
pstA
pstC
radA
rbpA
whiB
ybjG
),
quorum
sensing
dppC
livM
luxS
sapF
polysaccharide
metabolic
process
rfbX
galE
zwf
opcA,
glgC
glgP
gtfA
)
may
be
In
addition,
17
weighted
gene
co-expression
network
(WGCNA)
modules
identified
two
them
positively
correlated
BR.
Metabolomic
amino
acids
amides;
organic
acids,
alcohols
esters;
sugar
(trehalose-6-phosphate,
uridine
diphosphategalactose,
diphosphate-N-acetylglucosamine)
main
during
These
indicate
response,
(QS),
EPS
production
are
essential
