Environmental Science and Pollution Research, Год журнала: 2023, Номер 31(4), С. 6258 - 6276
Опубликована: Дек. 26, 2023
Язык: Английский
The Science of The Total Environment, Год журнала: 2024, Номер 924, С. 171627 - 171627
Опубликована: Март 11, 2024
This study aimed to investigate the effect of soil pH change, and nitrogen amendment on ammonia oxidiser abundance comammox Nitrospira community composition. The experimental design used mesocosms placed in a temperature-controlled incubator for 90 days. A Templeton silt loam was as its physiochemical properties are typical region's dairy farms. results showed that clade B preferred natural (pH 6.1–6.2) with no applied nitrogen. Furthermore, synthetic urine (N700) decreased B. may have been because large amounts available N700 treatments inhibited growth Nitrospira. These suggest while present New Zealand farm soils, but their role nitrification very high environment under patch grazed pastures be limited. Further research is needed confirm this. In contrast comammox, AOB (dominated by Nitrosospira) responded positively application urine. response greatest (7.1), followed then low (4.9) soils. due difference availability. At pH, ammonia/ammonium equilibrium favours production. Calculated availability accurately predicted amoA gene abundance. Interestingly, AOA (which predominantly made up Thaumarchaeota group I.1b E) seemed prefer soils over pH. specific lineage present. did not respond
Язык: Английский
Процитировано
10
Frontiers in Microbiology, Год журнала: 2024, Номер 15
Опубликована: Май 14, 2024
Through the combined use of two nitrification inhibitors, Dicyandiamide (DCD) and chlorate with nitrogen amendment, this study aimed to investigate contribution comammox
Язык: Английский
Процитировано
7
Soil Biology and Biochemistry, Год журнала: 2023, Номер 189, С. 109273 - 109273
Опубликована: Дек. 8, 2023
In agricultural soils, nitrogen (N) fertilizer is typically applied as ammonium salts or urea, and undergoes microbially mediated oxidation. However, insights regarding substrate concentrations nitrifiers are exposed to in the zones immediately surrounding N granules remain unexamined, well how this affects activity inhibition of nitrifier groups adapted different ranges ammonia (NH3) concentrations. To examine millimeter scale changes soil chemistry after sub-surface granule application with high spatiotemporal resolution, we a newly developed NH3 pH planar optical sensor (optode) system. With system, visualized situ subsurface production, diffusion, associated shifts an following (from 5 min up 65.5 h) chloride (NH4Cl) urea laboratory study. Ammonia from both NH4Cl diffused 1.5 2 cm reached maximum >49 >130 ppmv, respectively. Spatially informed destructive subsampling (0.5–2 granules) revealed that nitrate accumulation was greatest closest (0.5–1 cm) granules, while nitrite not detected. contrast, resulted accumulation, highest detected furthest (1.5–2 granules. Urea hydrolysis also caused significant localized increase, circular elevated zone migrating outwards leaving decreased at center. Transcription ammonia-oxidizing bacterial amoA gene areas there were no differences transcription increasing distance Ammonia-oxidizing archaea comammox transcripts This study first directly visualize small-scale concentration relate these nitrification activity.
Язык: Английский
Процитировано
13
Bioresource Technology, Год журнала: 2023, Номер 385, С. 129353 - 129353
Опубликована: Июнь 17, 2023
Язык: Английский
Процитировано
12
Chemosphere, Год журнала: 2024, Номер 358, С. 142093 - 142093
Опубликована: Апрель 26, 2024
Язык: Английский
Процитировано
5
Journal of Environmental Management, Год журнала: 2024, Номер 367, С. 121939 - 121939
Опубликована: Июль 27, 2024
Язык: Английский
Процитировано
4
Applied Soil Ecology, Год журнала: 2024, Номер 199, С. 105417 - 105417
Опубликована: Апрель 20, 2024
The response of complete ammonia-oxidizing (comammox) bacteria to variable nitrogen (N) inputs and nitrification inhibitors (NIs) remains poorly understood. We conducted two experiments, in a soil where both clade A B comammox Nitrospira were present, identify the type (ammonium sulphate vs. urea) level N-fertilization (0–500 mg-N-kg‐1d.w. soil) that are conducive growth comammox, compared canonical microorganisms (AOM), subsequently assess their three commercial (DCD, nitrapyrin, DMPP) new potent (ethoxyquin) NI under these favourable N fertilization regimes. highest concentration ammonium stimulated AOM which showed dose-dependent increase by sources. DMPP primarily, DCD, imposed more persistent inhibition on than nitrapyrin ethoxyquin, result did not concur with persistence NIs (DMPP was least among NIs) but mostly driven selective inhibitory activity (AOB) prevailed studied soil. AOB DCD complemented proliferation archaea (AOA). induced significant changes communities. Comammox enriched depleted ethoxyquin. (i) releasing AOA from competition allows them proliferate efficiently, even high inorganic conditions; (ii) responsive input NIs.
Язык: Английский
Процитировано
3
Frontiers in Microbiology, Год журнала: 2025, Номер 15
Опубликована: Янв. 15, 2025
The contrasting response of AOA, AOB, and comammox Nitrospira amoA transcript abundance to temperature, moisture, nitrogen was investigated using soil microcosms. treatments were selected represent conditions typically found in a New Zealand (NZ) dairy farm. AOB dominated all synthetic urine treated soils. Peak positively correlated with estimated ammonia availability. While gDNA nitrification rate trends similar. AOA strongly influenced by temperature. At 20°C, peak averaged over 1 order magnitude higher than at 8°C. Within the community member Nitrosocosmicus clade ammonium concentrations. presence relative increase an high environment poses interesting contrast current scientific opinion NZ. Comammox showed no correlation moisture. This suggests that previously associations are more complex originally thought. Further research is required determine drivers moisture environment. Overall, these results indicate main farm
Язык: Английский
Процитировано
0
Agronomy, Год журнала: 2025, Номер 15(2), С. 255 - 255
Опубликована: Янв. 21, 2025
The application of nitrification inhibitors (NIs) is an effective way to reduce soil nitrogen (N) losses and increase crop N uptake. Yet, the efficacy NIs commonly varies with dosages, systems environmental conditions. Hence, clarifying suitable type dosage extremely important for structuring best management regime at a regional scale. Here, based on microcosm experiments, we evaluated influence three widely used [Dicyandiamide, DCD; 3,4-Dimethylpyrazole phosphate, DMPP; 2-chloro-6-(trichloromethyl) pyridine, Nitrapyrin] activity intensively cultivated greenhouse soil. results showed that both DCD DMPP imposed transient inhibition (less than five days) regardless dosages applied, and, contrary, Nitrapyrin presented persistent suppression, longer duration action by higher dosage. Accordingly, incorporation 2% applied rate (w/w) recommendable local intensive production. Further, assessed various (0%, 0.25%, 0.5%, 5%) abundance community groups ammonia oxidizers [i.e., ammonia-oxidizing archaea (AOA), bacteria (AOB) completely (Comammox Nitrospira)] qPCR high-throughput amplicon sequencing. strongly lowered AOB Comammox Nitrospira abundances their richness even lowest also significantly altered structure all tested oxidizers, average relative some major members (i.e., Nitrososphaerales Clade Nitrososphaera, A, Nitrosospira briensis Clade, multiformis A.2 A-associated) obviously responded incorporation. Overall, our findings indicated were more sensitive as compared AOA. obtained here highlight importance optimizing fertilization in vegetable inhibits probably suppressing level herein.
Язык: Английский
Процитировано
0
Journal of Water Process Engineering, Год журнала: 2025, Номер 75, С. 107918 - 107918
Опубликована: Май 10, 2025
Язык: Английский
Процитировано
0