Development of Candida autochthonous starter for cigar fermentation via dissecting the microbiome

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Фев. 24, 2023

Introduction The main goal of tobacco fermentation technology is to minimize the alkaloid content while improving flavor substance content. Methods This study revealed microbial community structure and their metabolic functions during cigar leaf by high-throughput sequencing correlation analysis, evaluated performance functional microbes based on in vitro isolation bioaugmentation fermentation. Results relative abundance Staphylococcus Aspergillus increased first but then decreased fermentation, would occupy dominant position bacterial fungal communities, respectively, 21st day. Correlation analysis predicted that , Filobasidium could contribute formation saccharide compounds, Bacillus might have degradation effects nitrogenous substances. In particular, Candida as a co-occurring taxa biomarker later stage not only degrade substrates synthesize substances, also maintaining stability community. Moreover, inoculation, it was found parapsilosis metapsilosis significantly reduce alkaloids increase components leaves. Discussion validated critical role leaves through which help guide development starters directional regulation quality.

Язык: Английский

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Microbial and enzymatic changes in cigar tobacco leaves during air-curing and fermentation

Applied Microbiology and Biotechnology, Год журнала: 2023, Номер 107(18), С. 5789 - 5801

Опубликована: Июль 17, 2023

Metabolic enzyme activity and microbial composition of the air-curing fermentation processes determine quality cigar tobacco leaves (CTLs). In this study, we reveal evolution dominant microorganisms community structure at different stages CTLs. The results showed that changes in metabolic enzymes occurred mainly during phase, with polyphenol oxidase (PPO) being most active browning phase. Pseudomonas, Bacteroides, Vibrio, Monographella, Bipolaris, Aspergillus were key processes. Principal coordinate analysis revealed significant separation communities between phases. Redundancy bacteria such as Proteobacteria, Firmicutes, Bacteroidota, Acidobacteriota fungi Ascomycota Basidiomycota correlated temperature humidity. Bacteria act sugar metabolism, lipid amino acid while degrade lignin, cellulose, pectin through saprophytic action. Spearman correlation network Actinobacteria bacterial taxa, Dothideomycetes, Sordariomycetes, Eurotiomycetes fungal taxa. This research provides basis for improving cigars by KEY POINTS: • Changes POD PPO control color change CTLs stage. Aspergillus, Vibrio play an important role fermentation. Environmental humidity affect process, whereas are associated

Язык: Английский

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Metabolomics analysis of flavor differences in Shuixian (Camellia sinensis) tea from different production regions and their microbial associations

Food Chemistry, Год журнала: 2024, Номер 443, С. 138542 - 138542

Опубликована: Янв. 23, 2024

Язык: Английский

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Analyzing microbial community and volatile compound profiles in the fermentation of cigar tobacco leaves

Applied Microbiology and Biotechnology, Год журнала: 2024, Номер 108(1)

Опубликована: Фев. 29, 2024

Variations in industrial fermentation techniques have a significant impact on the of cigar tobacco leaves (CTLs), consequently influencing aromatic attributes resulting cigars. The entire process CTLs can be categorized into three distinct phases: phase 1 (CTLs prior to moisture regain), 2 post-moisture regain and pile fermentation), 3 after drying). These phases were determined based dynamic changes microbial community diversity. During 2, there was rapid increase total acid content, which facilitated proliferation Aerococcus, bacterial genus capable utilizing reducing sugars, malic acid, citric present leaves. In contrast, fungal microorganisms exhibited relatively stable response with Aspergillus, Alternaria, Cladosporium being dominant groups throughout stages. Bacterial genera found more closely associated variations volatile compounds during compared microorganisms. This association ultimately resulted higher levels aroma components CTLs, thereby improving overall quality findings reinforce significance shaping CTL provide valuable insights for future efforts artificial regulation secondary CTLs. KEY POINTS: • Industrial processes communities. Moisture content influence succession fermentation. strongly CTLs' aldehyde ketone flavors over fungi.

Язык: Английский

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Correlation study on microbial communities and volatile flavor compounds in cigar tobacco leaves of diverse origins

Applied Microbiology and Biotechnology, Год журнала: 2024, Номер 108(1)

Опубликована: Фев. 26, 2024

To elucidate the significant influence of microorganisms on geographically dependent flavor formation by analyzing microbial communities and volatile compounds (VFCs) in cigar tobacco leaves (CTLs) obtained from China, Dominica, Indonesia. Microbiome analysis revealed that predominant bacteria CTLs were Staphylococcus, Aerococcus, Pseudomonas, Lactobacillus, while fungi Aspergillus, Wallemia, Sampaiozyma. The different origins differed to some extent, diversity abundance greater than fungi. Metabolomic 64 VFCs identified, mainly ketones, which 23 could be utilized identify geographical CTLs. Sixteen with OAV 1, including cedrol, phenylacetaldehyde, damascone, beta-damascone, beta-ionone, play important roles shaping profile origins. Combined correlation analysis, bacterial more closely related key favored a positive correlation. Bacillus, Vibrio, Sphingomonas main flavor-related bacteria. study demonstrated essential for qualities CTLs, provided theoretical reference control technology. KEY POINTS: • It is high determine methylerythritol phosphate (MEP) pathway carotenoid synthesis are metabolic pathways Microbial interactions flavor, contributing

Язык: Английский

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Integrated characterization of filler tobacco leaves: HS–SPME–GC–MS, E-nose, and microbiome analysis across different origins

Bioresources and Bioprocessing, Год журнала: 2024, Номер 11(1)

Опубликована: Янв. 18, 2024

Abstract This study delves into the aroma characteristics and microbial composition of filler tobacco leaves (FTLs) sourced from six distinct cigar-growing regions within Yunnan, China, following standardized fermentation. An integrated approach using gas chromatography-mass spectrometry (GC–MS), electronic nose (E-nose), microbiome analysis was employed for comprehensive profiling. Results derived Linear Discriminant Analysis (LDA) E-nose data confirmed presence notable variability in flavor substance profiles among FTLs regions. Additionally, GC–MS used to discern disparities volatile organic compound (VOC) distribution across these regions, identifying 92, 81, 79, 58, 69, 92 VOCs each respective sample set. Significantly, 24 emerged as pivotal determinants contributing heterogeneity diverse origins, indicated by Variable Importance Projection (VIP) analysis. Furthermore, distinctions free amino acid content chemical constituents were observed FTLs. Of noteworthy significance, solanone, isophorone, durene, (-)-alpha-terpineol, 2,3'-bipyridine exhibited strongest correlations with data, fungal microorganisms exerting a more pronounced influence on metabolites, elucidated through two-way orthogonal partial least-squares (O2PLS) modeling. These findings provide theoretical technical basis accurately evaluating synchronization aromas fermentation processes, they will enhance quality fermented foster growth domestic cigar industry ultimately. Graphical

Язык: Английский

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Analysis of differences in aroma and sensory characteristics of the mainstream smoke of six cigars

Heliyon, Год журнала: 2024, Номер 10(4), С. e26630 - e26630

Опубликована: Фев. 1, 2024

Cigars have unique aroma and style characteristics. In order to clarify the differences of components between domestic imported cigars material basis stylistic characteristics different cigars, gas chromatography-mass spectrometry (GC-MS) sensory evaluation were used compare analyze in mainstream smoke four two cigars. The GC-MS results showed that a total 97 measured six types similar, but there their contents. comparison with those had suitable nicotine content, higher contents phytol, neophytadiene, 3-methylpentanoic acid, (+)-δ-cadinene. To further explore data combined chemometrics screen out 14 key based on P-value (P) < 0.05, Variable Importance Projection (VIP) > 1, Aroma Activity Values (OAV) 1. each cigar obtained, Snow Dream No. 5: cedrol; Wangguan Guocui: 6-methyl-5-hepten-2-one, pyridine, 2-ethyl-6-methylpyrazine; General Achileus 3: p-cresol, 2-methylbutyraldehyde, methyl cyclopentenolone; Montecristo 4: cedrol, guaiacol, 4-vinylguaiacol, Romeo y Julieta Wide Churchills: 2,6-dimethylpyrazine, 2-ethyl-6-methylpyrazine, 2-heptanone, phenethyl alcohol; Great Wall 2: alcohol, geranylacetone, cyclopentenolone, dihydroactinidiolide. odor descriptors these compounds consistent profiles prominent senses cigar. This experiment initially explored composition provided support quality improvement

Язык: Английский

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Controlling mildew of tobacco leaf by Bacillus amyloliquefaciens ZH-2 and its effect on storage quality of tobacco leaf

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 12, 2025

Язык: Английский

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Interaction analysis of tobacco leaf microbial community structure and volatiles flavor compounds during cigar stacking fermentation

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Авг. 10, 2023

Introduction Cigar stacking fermentation is a key step in tobacco aroma enhancement and miscellaneous gas reduction, which both have great influence on increasing cigar flavor improving industrial availability. Methods To analyze the effect of leaf (CTLs) microbial community volatiles compounds (VFCs), this study used multi-omics technology to reveal changes structure VFCs different varieties during fermentation, addition exploring interaction mechanism microbiome VFCs. Results The results showed that dominant compositions CTL were similar, included Staphylococcus , Corynebacterium 1 Aerococcus Aspergillus . These microbes mainly affected characteristic microorganisms CTLs through interactions, thereby influencing transformation Characteristic such as Trichothecium Trichosporon Thioalkalicoccus Jeotgalicoccus found positively correlate with like megastigmatrienone 4, pyrazine, tetramethyl-, geranyl acetone, 2-undecanone, 6,10-dimethyl-, respectively. This turn sensory quality CTLs. Discussion provides theoretical support for analysis aroma, development agents fermentation.

Язык: Английский

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Changes in physicochemical properties and microbial community succession during leaf stacking fermentation

AMB Express, Год журнала: 2023, Номер 13(1)

Опубликована: Ноя. 22, 2023

Leaf stacking fermentation involves enzymatic actions of many microorganisms and is an efficient environmentally benign process for degrading macromolecular organic compounds. We investigated the dynamics metabolite profiles, bacterial fungal communities their interactions during using cigar leaves from three geographic regions. The results showed that contents total sugar, reducing starch, cellulose, lignin, pectin, polyphenol protein in tobacco was significantly decreased fermentation. Notably, furfural, neophytadiene, pyridine, benzyl alcohol, geranylacetone, 3-hydroxy-2-butanone, N-hexanal, 3-Methyl-1-butanol 2,3-pentanedione were important features volatile aroma compounds α-diversity fungi bacteria initially increased then An analysis variance microbial diversity influenced by stages growing locations, which all had greater impacts on α- β-diversity than Microbiome profiling identified several core including Sphingomonas, Bacillus, Staphylococcus, Pseudomonas, Ralstonia, Massilia Fibrobacter. Fungal biomarkers included Aspergillus, Penicillium, Fusarium, Cladosporium Trichomonascus. Interestingly, molecular ecological networks taxa significant correlations with metabolic enzymes physicochemical properties; jointly participated carbohydrate nitrogen compound chemosynthesis processes These studies provide insights into coupling material conversion community succession leaf

Язык: Английский

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