Unseen Weapons: Bacterial Extracellular Vesicles and the Spread of Antibiotic Resistance in Aquatic Environments

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(6), С. 3080 - 3080

Опубликована: Март 7, 2024

This paper sheds light on the alarming issue of antibiotic resistance (ABR) in aquatic environments, exploring its detrimental effects ecosystems and public health. It examines multifaceted role use aquaculture, agricultural runoff, industrial waste fostering development dissemination resistant bacteria. The intricate interplay between various environmental factors, horizontal gene transfer, bacterial extracellular vesicles (BEVs) accelerating spread ABR is comprehensively discussed. Various BEVs carrying genes like blaCTX-M, tetA, floR, sul/I, as well their contribution to dominance multidrug-resistant bacteria, are highlighted. potential both a threat tool combating explored, with promising strategies targeted antimicrobial delivery systems probiotic-derived EVs holding significant promise. underscores urgency understanding environments. By unraveling these unseen weapons, we pave way for developing effective mitigate ABR, advocating multidisciplinary approach that includes stringent regulations, enhanced wastewater treatment, adoption sustainable practices aquaculture.

Язык: Английский

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Characterisation of microbial communities and quantification of antibiotic resistance genes in Italian wastewater treatment plants using 16S rRNA sequencing and digital PCR

The Science of The Total Environment, Год журнала: 2024, Номер 933, С. 173217 - 173217

Опубликована: Май 13, 2024

The spread of antibiotic resistant bacteria (ARB) and resistance genes (ARGs) in humans, animals environment is a growing threat to public health. Wastewater treatment plants (WWTPs) are crucial mitigating the risk environmental contamination by effectively removing contaminants before discharge. However, persistence ARB ARGs even after challenge for management water system. To comprehensively assess antimicrobial dynamics, we conducted one-year monitoring study three WWTPs central Italy, both influents effluents. We used seasonal sampling analyze microbial communities 16S rRNA, as well determine prevalence behaviour major (sul1, tetA, bla

Язык: Английский

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Linking antibiotic resistance gene patterns with advanced faecal pollution assessment and environmental key parameters along 2300 km of the Danube River

Water Research, Год журнала: 2024, Номер 252, С. 121244 - 121244

Опубликована: Янв. 31, 2024

The global spread of antimicrobial resistance (AMR) in the environment is a growing health threat. Large rivers are particular concern as they highly impacted by wastewater discharge while being vital lifelines serving various human needs. A comprehensive understanding occurrence, and key drivers AMR along whole river courses largely lacking. We provide holistic approach studying spatiotemporal patterns hotspots antibiotic genes (ARGs) 2311 km navigable Danube River, combining longitudinal temporal monitoring campaign. integration advanced faecal pollution diagnostics environmental chemical parameters allowed linking ARG concentrations to major sources explaining observed patterns. Nine markers, including conferring five different classes clinical relevance, one integrase gene were determined probe-based qPCR. All targets could be quantified River water, with intI1 sul1 ubiquitously abundant, qnrS, tetM, blaTEM intermediate abundance blaOXA-48like, blaCTX-M-1group, blaCTX-M-9group blaKPC rare occurrence. Human from municipal discharges was dominant factor shaping River. Other significant correlations specific ARGs discharge, certain metals pesticides. In contrast, not associated but already established water microbiome. Animal contamination detected only sporadically correlated sampling set. During monitoring, an extraordinary hotspot identified emphasizing variability within natural waters. This study provides first baseline lays foundation for future trends evaluating potential reduction measures. applided proved valuable methodological contribution towards better occurrence AMR.

Язык: Английский

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Antibiotic resistant bacteria and genes (ARB and ARG) in water and sewage treatment units: A review

Environmental Nanotechnology Monitoring & Management, Год журнала: 2024, Номер 21, С. 100941 - 100941

Опубликована: Март 22, 2024

Язык: Английский

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Riverine antibiotic resistome along an anthropogenic gradient

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Фев. 26, 2025

The introduction of antibiotic-resistant bacteria into riverine systems through the discharge wastewater treatment plant (WWTP) effluent and agricultural waste poses significant health risks. Even when not pathogenic, these can act as reservoirs for antibiotic resistance genes (ARGs), transferring them to pathogens that infect humans animals. In this study, we used fluorescence in situ hybridization, qPCR, metagenomics investigate how anthropogenic activities affect microbial abundance resistome along Holtemme River, a small river Germany, from near-pristine human-impacted sites. Our results showed higher bacterial abundance, greater absolute relative ARGs, more diverse ARG profile at impacted Overall, profiles sites reflected usage with conferring drug classes such beta-lactams, aminoglycosides, folate biosynthesis inhibitors, tetracyclines. There were also variations Notably, there was high oxacillin gene OXA-4 downstream site river. metagenome assembly, associated contig homologous plasmids previously identified members Thiotrichaceae. likely in-situ host putative plasmid close Thiolinea (also known Thiothrix) eikelboomii, prominent member WWTP microbiomes worldwide. show WWTPs introduce environment shuttle clinically relevant ARG.

Язык: Английский

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Lotic Ecosystem Sediment Microbial Communities’ Resilience to the Impact of Wastewater Effluents in a Polluted European Hotspot—Mureș Basin (Transylvania, Romania)

Water, Год журнала: 2024, Номер 16(3), С. 402 - 402

Опубликована: Янв. 25, 2024

The aim of this study was to assess the impact WWTP effluents on sediment microbial communities throughout Mureș River. This shows existence an ecological equilibrium between effluent disruptors and resilience River microbiomes, a fact that suggests river’s stable/balanced status in regard, partly due communities’ local effluents. High-throughput 16S bacterial metabarcoding used evaluate sediment. Due lotic system’s sensitivity environmental changes, we assumed dependency these community structures functions abiotic parameters. results show that, although are equally diverse three locations (upstream WWTP, effluents, downstream WWTP), there is difference structure upstream samples samples, while contain mixture communities. Just sediment, influenced by specific input from effluents; nevertheless, river microbiome resilient able further recover its natural composition, as evidenced similarity at all locations. demonstrates capacity indicates status, part Based findings, monitoring system should be implemented here future.

Язык: Английский

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Genome-centric analyses of 165 metagenomes show that mobile genetic elements are crucial for the transmission of antimicrobial resistance genes to pathogens in activated sludge and wastewater

Microbiology Spectrum, Год журнала: 2024, Номер 12(3)

Опубликована: Янв. 30, 2024

ABSTRACT Wastewater is considered a reservoir of antimicrobial resistance genes (ARGs), where the abundant antimicrobial-resistant bacteria and mobile genetic elements facilitate horizontal gene transfer. However, prevalence extent these phenomena in different taxonomic groups that inhabit wastewater are still not fully understood. Here, we determined presence ARGs metagenome-assembled genomes (MAGs) evaluated risks MAG-carrying potential human pathogens. The to be transmitted horizontally or vertically was also determined. A total 5,916 MAGs (completeness >50%, contamination <10%) were recovered, covering 68 phyla 279 genera. dereplicated into 1,204 genome operational units (gOTUs) as proxy for species ( average nucleotide identity >0.95). dominant ARG classes detected bacitracin, multi-drug, macrolide-lincosamide-streptogramin (MLS), glycopeptide, aminoglycoside, 10.26% them located on plasmids. main hosts belonged Escherichia , Klebsiella Acinetobacter Gresbergeria Mycobacterium Thauera . Our data showed 253 carried virulence factor (VFGs) divided 44 gOTUs, which 45 carriers ARGs, indicating pathogens ARGs. Alarmingly, MAG assigned coli contained 159 VFGs, 95 chromosomes 10 In addition shedding light individual recovered from activated sludge wastewater, our study demonstrates workflow can identify complex microbial communities. IMPORTANCE Antimicrobial (AMR) threatens health humans, animals, natural ecosystems. study, an analysis 165 metagenomes revealed antibiotic-targeted alteration, efflux, inactivation most prevalent AMR mechanisms. We identified several genera correlated with multiple including Nitrospira Ottowia Pseudomonas could have significant implications transmission. abundance bacA mexL aph(3")-I calls their urgent management wastewater. approach applied ecosystems assess risk containing findings highlight importance managing help design measures reduce transmission evolution systems.

Язык: Английский

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Ecotoxicity of five veterinary antibiotics on indicator organisms and water and soil communities

Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 274, С. 116185 - 116185

Опубликована: Март 14, 2024

This study explores the environmental effects of five common veterinary antibiotics widely detected in environment, (chlortetracycline,CTC; oxytetracycline,OTC; florfenicol,FF; neomycin, NMC; and sulfadiazine, SDZ) on four bioindicators: Daphnia magna, Vibrio fischeri, Eisenia fetida, Allium cepa, representing aquatic soil environments. Additionally, microbial communities characterized through 16 S rRNA gene sequencing from a river natural were exposed to assess changes population growth metabolic profiles using Biolog EcoPlates™. Tetracyclines are harmful fisheri (LC

Язык: Английский

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Nature-based solutions for antibiotics and antimicrobial resistance removal in tertiary wastewater treatment: Microbiological composition and risk assessment

Water Research, Год журнала: 2024, Номер 261, С. 122038 - 122038

Опубликована: Июль 2, 2024

This field-scale study evaluates the seasonal effectiveness of employing nature-based solutions (NBSs), particularly surface flow and horizontal subsurface constructed wetland configurations, as tertiary treatment technologies for removal antibiotics (ABs) antibiotic resistance genes (ARGs) compared to a conventional involving UV chlorination. Out 21 monitored ABs, 13 were detected in influent three wastewater treatments, with concentrations ranging from 2 1218 ng·L-1. The ARGs sul1 dfrA1 exhibited 1 × 105 9 106 copies/100 mL. NBSs better at reducing ABs (average 69 88 %) (2–3 log units) system 36 39 % no both seasons. Taxonomic compositions water samples shifted wastewater-impacted communities (Actinomycetota Firmicutes) combination plant rhizosphere-associated river NBS effluents (Alphaproteobacteria). In contrast, technology showed substantial differences community composition. Moreover, substantially reduced ecotoxicological risk assessment (cumulative RQs). Furthermore, RQs) by an average over 70 across seasons, whereas benchmark only achieved 6 reduction. conclusion, present robust alternative minimizing discharge into bodies.

Язык: Английский

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Degradation of plasmid-mediated resistance genes in poultry slaughterhouse wastewater employing a UV/H2O2 process: A metagenomic approach

Chemosphere, Год журнала: 2025, Номер 372, С. 144109 - 144109

Опубликована: Янв. 17, 2025

Язык: Английский

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