Ring-opening reaction of maleic anhydride with an amino-end derivative of sulfobetaine methacrylate for effective, versatile and stable fabrication of bacteria and blood-resistant porous polymeric films

Journal of the Taiwan Institute of Chemical Engineers, Год журнала: 2025, Номер 168, С. 105947 - 105947

Опубликована: Янв. 5, 2025

Язык: Английский

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Catalytic insights into laccase for sustainable remediation of multifaceted pharmaceutically active micropollutants from water matrices: A state-of-art review

Journal of Water Process Engineering, Год журнала: 2025, Номер 70, С. 106901 - 106901

Опубликована: Янв. 14, 2025

Язык: Английский

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Molecular epidemiology and mechanisms of carbapenem and colistin resistance in Klebsiella and other Enterobacterales from treated wastewater in Croatia

Environment International, Год журнала: 2024, Номер 185, С. 108554 - 108554

Опубликована: Март 1, 2024

Among the most problematic bacteria with clinical relevance are carbapenem-resistant Enterobacterales (CRE), as there very limited options for their treatment. Treated wastewater can be a route release of these into environment and population. The aim this study was to isolate CRE from treated Zagreb treatment plant determine phenotypic genomic characteristics. A total 200 suspected were isolated, 148 which confirmed by MALDI-TOF MS. predominant species Klebsiella spp. (n = 47), followed Citrobacter 40) Enterobacter cloacae complex (cplx.) 35). All isolates carbapenemase producers multidrug-resistant phenotype. Using multi-locus sequence typing whole-genome sequencing (WGS), 18 different types identified among isolates, 14 associated human-associated clones. virulence gene analysis sequenced 7) revealed potential pathogenicity. PCR WGS showed that frequent genes in K. pneumoniae blaOXA-48 blaNDM-1, frequently occurred together, while blaKPC-2 together blaNDM-1 mainly detected oxytoca, E. cplx. Colistin resistance observed 40 % 57 isolates. Underlying mechanisms include known potentially novel intrinsic (point mutations pmrA/B, phoP/Q, mgrB crrB genes) acquired (mcr-4.3 gene). mcr-4.3 first time is probably located on conjugative IncHI1B plasmid. In addition, 13 various other clinically relevant antibiotics well plasmids possibly genes. Our demonstrates important role municipal plays harboring spreading enterobacterial pathogens resistant last-resort antibiotics.

Язык: Английский

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The COVID-19 Pandemic Impact of Hospital Wastewater on Aquatic Systems in Bucharest

Water, Год журнала: 2024, Номер 16(2), С. 245 - 245

Опубликована: Янв. 10, 2024

The COVID-19 pandemic reshaped the global response to a pandemic, including way of using chemical compounds such as disinfectants and antibiotics. large-scale use antibiotics during caused environmental pressure not only due chemicals themselves but also their effect on bacterial communities, inducing resistance changing population structure especially in aquatic environments. dissemination fecal bacteria, antibiotic-resistant pathogens from hospital wastewater into environment, via treatment plants (WWTPs), triggered premises major public health issue. Rivers flowing through cities are natural streams for WWTP discharges, they directly bear impact anthropic activities, disseminating domestic industrial pollution over large areas. aim present study was assess microbiological municipal wastewaters well streams, covering pre-to post-COVID-19 period time. results indicated that had direct quality environment an excessive disinfectants. In addition, constant presence antibacterial increased rate selection induced structural changes communities systems.

Язык: Английский

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Analysis of the transmission chain of carbapenem-resistant Enterobacter cloacae complex infections in clinical, intestinal and healthcare settings in Zhejiang province, China (2022–2023)

The Science of The Total Environment, Год журнала: 2024, Номер 920, С. 170635 - 170635

Опубликована: Фев. 8, 2024

Considerable attention is given to intensive care unit-acquired infections; however, research on the transmission dynamics of multichain carbapenemase-resistant Enterobacter cloacae complex (CRECC) outbreaks remains elusive. A total 118 non-duplicated CRECC strains were isolated from clinical, intestinal, and hospital sewage samples collected Zhejiang province China during 2022–2023. 64 samples, their prevalence increased 10.0 % (95 confidence interval, CI = 0.52–45.8 %) in 2022 63.6 31.6–87.6 2023. Species-specific identification revealed that hormaechei was predominant species this study (53.4 %, 95 44.0–62.6 %). The antimicrobial susceptibility profiles indicated all conferred high-level resistance β-lactam antibiotics, ceftacillin/avibactam, polymyxin. Furthermore, exhibited β-lactams, quinolones, fosfomycin, with a higher colistin rate observed (67.2 54.2–78.1 Several antibiotic genes identified strains, including Class carbapenemases (blaKPC-2) B (blaNDM-1/blaIMP), but not D carbapenemases. WGS analysis showed majority carried carbapenemase-encoding genes, blaNDM-1 being most prevalent (86.9 77.4–92.9 sequence typing belonged diverse types (STs), among which ST418 blaNDM-positive strain. high risk carbapenemase-producing E. blaNDM-harboring IncFIB-type plasmid (81.4 72.9–87.7 detected emphasized study. This provides valuable insights into prevalence, resistance, genomic characteristics, populations environments. clonal relatedness sporadic supporting inter-hospital transmission.

Язык: Английский

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Inactivation of antibiotic-resistant bacteria Escherichia coli by electroporation

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Янв. 25, 2024

Introduction In modern times, bacterial infections have become a growing problem in the medical community due to emergence of antibiotic-resistant bacteria. fact, overuse and improper disposal antibiotics led resistance presence such bacteria wastewater. Therefore, it is critical develop effective strategies for dealing with Electroporation has been found be one most promising complementary techniques inactivation because against wide range bacteria, non-chemical highly optimizable. Many studies demonstrated electroporation-assisted but rarely clinical or resistant these used study. motivation our study was use treatment regimen that combines electroporation inactivate Methods We separately combined two (tetracycline chloramphenicol) which are (with different mode) electric pulses. three concentrations (40, 80 150 µg/ml tetracycline 100, 500 2000 chloramphenicol, respectively) four field strengths (5, 10, 15 20 kV/cm) electroporation. Results discussion Our results show effectively enhances effect inactivates The rate chloramphenicol increase strength pulsed and/or concentration antibiotic. addition, we longer lasting (up 24 hours), making vulnerable considerable time. present work provides new insights into aquatic environment.

Язык: Английский

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Predictive statistical models for monitoring antimicrobial resistance spread in the environment using Apis mellifera (L. 1758) colonies

Environmental Research, Год журнала: 2024, Номер 248, С. 118365 - 118365

Опубликована: Янв. 30, 2024

Язык: Английский

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Presence and Persistence of ESKAPEE Bacteria before and after Hospital Wastewater Treatment

Microorganisms, Год журнала: 2024, Номер 12(6), С. 1231 - 1231

Опубликована: Июнь 19, 2024

The metagenomic surveillance of antimicrobial resistance in wastewater has been suggested as a methodological tool to characterize the distribution, status, and trends antibiotic-resistant bacteria. In this study, cross-sectional collection samples hospital-associated raw treated were obtained from February March 2020. Shotgun sequencing bioinformatic analysis performed bacterial abundance gene analysis. main phyla found all follows: Proteobacteria, Bacteroides, Firmicutes, Actinobacteria. At species level, ESKAPEE bacteria such E. coli relative decreased between wastewater, but S. aureus, A. baumannii, P. aeruginosa increased, did persistence K. pneumoniae both wastewater. A total 172 different ARGs detected; blaOXA, blaVEB, blaKPC, blaGES, mphE, mef, erm, msrE, AAC(6′), ant(3″), aadS, lnu, PBP-2, dfrA, vanA-G, tet, sul at highest persistence. This study demonstrates ability survive tertiary treatment processes hospital well clinically important genes that are spreading environment.

Язык: Английский

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Treated wastewater: A hotspot for multidrug- and colistin-resistant Klebsiella pneumoniae

Environmental Pollution, Год журнала: 2024, Номер 359, С. 124598 - 124598

Опубликована: Июль 23, 2024

Wastewater treatment plants are hotspots for the release of antimicrobial resistant pathogenic bacteria into aquatic ecosystems, significantly contributing to cycle resistance. Special attention should be paid ESKAPE bacteria, which have been identified as high-priority targets control measures. Among them, Klebsiella pneumoniae is particularly noteworthy. In this study, we collected wastewater samples from inlet, sedimentation tank, and effluent water a plant in June, July, October, November 2018. We detected characterized 42 K. strains using whole genome sequencing (15 8 19 effluent). Additionally, were tested their resistance phenotype. Using no distinct patterns observed terms genetic profiles. All tetracycline, meanwhile 60%, 47%, 37.5% isolated effluent, respectively, multidrug resistant. Some isolates also colistin, nearly all positive eptB arnT genes, associated with polymyxin Various genes linked mobile elements, they did not correlate virulence groups or defense systems. Overall, our results, although quantitative, highlight that strains, including those colistin genetically unrelated, being discharged ecosystems plants. This suggests necessity monitoring aimed at characterizing these bacteria.

Язык: Английский

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Metagenomic Profiling of Internationally Sourced Sewage Influents and Effluents Yields Insight into Selecting Targets for Antibiotic Resistance Monitoring

Environmental Science & Technology, Год журнала: 2024, Номер 58(37), С. 16547 - 16559

Опубликована: Сен. 4, 2024

It has been debated whether wastewater treatment plants (WWTPs) primarily act to attenuate or amplify antibiotic resistance genes (ARGs). However, ARGs are highly diverse with respect their mechanisms, mobilities, and taxonomic hosts therefore behavior in WWTPs should not be expected universally conserved. We applied metagenomic sequencing influent effluent samples from 12 international classify the of specific entering exiting WWTPs. In total, 1079 different originating a variety bacteria were detected. This included that could mapped assembled scaffolds corresponding nine human pathogens. While relative abundance (per 16S rRNA gene) decreased during at 11 sampled absolute mL) all WWTPs, increases observed for 40% detected 12th WWTP. Also, mobile genetic elements (MGE) increased treatment, but fraction known transmissible between species decreased, thus demonstrating MGE prevalence may generally indicative an increase ARGs. A distinct conserved resistome was documented both across samples, suggesting well-functioning loads. work helps inform strategies surveillance resistance, highlighting utility tracking as indicators performance risk reduction.

Язык: Английский

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