Engineering Bacillus licheniformis as industrial chassis for efficient bioproduction from starch
Bioresource Technology,
Год журнала:
2024,
Номер
406, С. 131061 - 131061
Опубликована: Июль 1, 2024
Язык: Английский
Characterization of a novel levansucrase produced by Bacillus subtilis LG-4 isolated from traditional natto for biosynthesis of prebiotic lactosucrose
Food Bioscience,
Год журнала:
2025,
Номер
unknown, С. 106292 - 106292
Опубликована: Март 1, 2025
Язык: Английский
Hemp Seed Protein Hydrolysate Enriched with γ-Aminobutyric Acid and Peptides by Microbial Bioconversion
Fermentation,
Год журнала:
2024,
Номер
10(9), С. 483 - 483
Опубликована: Сен. 17, 2024
Hemp
seed
protein
(HSP),
a
by-product
of
hemp
oil
processing,
was
converted
into
functional
ingredient
enriched
with
γ-aminobutyric
acid
(GABA)
and
peptides
through
two-step
microbial
fermentation
process.
To
enhance
peptide
free
amino
production
from
HSP,
it
hydrolyzed
using
alkaline
protease
produced
by
Bacillus
subtilis
HA.
The
HSP
at
degree
40%
55
°C
for
24
h,
yielding
pH
6.55,
an
acidity
1.22%,
205.45
mg%
tyrosine
equivalents.
This
process
resulted
in
the
low
molecular-weight
peptides.
(<5000
Da)
total
content
branched-chain
acids
(leucine,
isoleucine,
valine)
were
6.78
mg/g
1.47
mg/g.
Subsequently,
hydrolysate
optimized
co-fermentation
lactic
bacteria
presence
5%
MSG
30
5
days.
serial
co-fermented
exhibited
GABA
33.98
viable
bacterial
count
9.51
log
CFU/mL
Lb.
plantarum
KS2020.
process,
combining
proteolysis
fermentation,
not
only
increased
but
also
promoted
accumulation,
positioning
as
promising
candidate
foods
potential
health
benefits.
Язык: Английский
Composition and Essential Nutrients of Cyanobacterium Meal Produced from Spirulina (Arthrospira platensis) Fermented by Yeast on Small-Scale
Case Studies in Chemical and Environmental Engineering,
Год журнала:
2024,
Номер
unknown, С. 100940 - 100940
Опубликована: Сен. 1, 2024
Язык: Английский
Characterization of alkaline protease enzyme produced from marine yeast Candida orthopsilosis AKB-1 and its applications
Folia Microbiologica,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 28, 2024
Язык: Английский
A novel alkali and thermotolerant protease from Aeromonas spp. retrieved from wastewater
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Окт. 29, 2024
Abstract
Enzymes
are
integral
to
numerous
industrial
processes,
with
a
growing
global
demand
for
various
enzyme
types.
Protease
enzymes,
in
particular,
have
proven
be
cost-effective,
stable,
and
compatible
alternatives
traditional
chemical
processes
both
environmental
applications.
In
this
study,
an
alkaline
protease-producing
strain
of
Aeromonas
spp.
was
isolated
from
wastewater
treatment
plant
Iran.
The
protease
production
confirmed
by
culturing
the
on
casein
agar
medium.
bacterium
identified
through
morphological,
biochemical,
16
S
rRNA
sequencing
analyses.
optimal
culture
medium
bacterial
growth
obtained
using
peptone,
salt,
yeast
extract,
galactose,
CaCl₂
at
initial
pH
8.
Maximum
achieved
after
20
h
incubation
40
°C.
To
partially
purify
enzyme,
supernatant
first
centrifuged,
precipitated
ammonium
sulfate,
followed
dialysis.
Zymography
revealed
one
type
during
growth.
purified
exhibited
activity
8.5
maximum
stability
9.
optimum
temperature
observed
50
°C,
100%
residual
retained
1
0
effect
metal
ions
assessed,
revealing
that
KCl
induced
most
significant
effects
(
p
<
0.0001)
activity.
Chemical
amino
acid
modifiers
inhibitors,
such
as
EDTA,
DEPSI,
IAA,
did
not
exhibit
inhibition.
contrast,
PMSF
HNBB
significantly
reduced
activity,
suggesting
could
classified
serine
protease.
also
demonstrated
high
presence
2%
SDS,
showing
no
signs
inactivation.
optimum,
thermal
stability,
resistance
SDS
produced
particularly
promising
characteristics
warrant
further
investigation.
Based
preliminary
tests
enzyme’s
characteristics,
can
recommended
applications,
pending
studies.
Язык: Английский