Microbial Pathogenesis, Год журнала: 2024, Номер 197, С. 107068 - 107068
Опубликована: Окт. 28, 2024
Язык: Английский
hLife, Год журнала: 2024, Номер unknown
Опубликована: Июнь 1, 2024
The emergence of hypervirulence (hv) and carbapenem resistance (CR) as distinct evolutionary directions for Klebsiella pneumoniae presents a significant threat in clinical settings. However, recent years, there has been growing identification K. strains that integrate both phenotypes, resulting severe outcomes. Carbapenem-resistant hypervirulent (CRhvKP) typically emerges through the acquisition plasmids carrying either virulence or CR-encoded genes by carbapenem-resistant pneumoniae. Furthermore, hybrid plasmid can confer combination CR hv. CRhvKP cause variety infections, including pneumonia, urinary tract bloodstream liver abscesses, other related conditions. While sequence type 11 (ST11) dominates majority China, molecular factors responsible success ST11 largely remain unknown. Here, we provide an overview current understanding variation distribution crucial determinants, mechanisms driving merging hv CR, potential influencing epidemiological CRhvKP. This research aims to contribute comprehensive complexities surrounding It is imperative underscore development therapies, precision medicine, vaccine strategies pivotal approaches effectively combating Considering widespread prevalence CRhvKP, prioritized, multifaceted approach encompassing infection control, active surveillance, innovative therapeutics essential.
Язык: Английский
Процитировано
10
Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15
Опубликована: Фев. 14, 2025
Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CRhvKP) poses a significant global health threat due to its enhanced virulence and resistance. This study analyzed 5,036 publicly available K. genomes from China (2005–2023), identifying 1,538 CRhvKP genomes, accounting for 44.6% of carbapenem-resistant isolates 69.5% isolates. Predominant carbapenemases included bla KPC (92.1%), with an increasing prevalence NDM OXA-48-like genes. Most (93.6%) carried both aerobactin yersiniabactin The genetic background showed high diversity, characterized by 36 sequence types (STs) 22 capsule types, high-risk endemic STs such as ST11, ST15, ST23 being predominant. demonstrated virulence, whereas ST11 more resistance genes but minimal presence iroBCDN A core genome MLST analysis revealed that 89.0% clustered into 131 clonal groups, indicating widespread dissemination, particularly in eastern China. CR hv plasmids, primarily IncF, IncH, IncR distinct community structures, plasmids demonstrating higher mobility diversity. Crucially, we identified 40 CR-hv convergent across five STs, likely resulting plasmid fusions, which have become increasingly prevalent over the last decade. Furthermore, chromosomal integration KPC-2 was detected, underscoring stable inheritance these traits. Class 1 Integrons were present 84.5% strains, most notably least ST23. These integrons harbored confer various antibiotics, including IMP VIM , their content varying different STs. highlights complexity, rapid China, emphasizing urgent need genomic surveillance targeted interventions mitigate posed multidrug-resistant strains.
Язык: Английский
Процитировано
1
Antibiotics, Год журнала: 2025, Номер 14(2), С. 206 - 206
Опубликована: Фев. 17, 2025
Introduction:Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) are resistant to third-generation cephalosporins (3GCs), carbapenems, colistin, tigecycline, making them a major public health priority, mainly within the developing world. However, their genomic epidemiology possible determinants of resistance remain be elucidated. Thus, this study aimed perform characterization E. K. pneumoniae, both which last-line antibiotics, isolated from humans, poultry, dairy farm environment Ecuador. Methods: This analyzed nine 3GC-resistant isolates harboring mcr-1 gene (six poultry farms, two human infections, one compost), together with ten colistin- carbapenem-resistant clinical samples. Results: The origin belonged ST609 phylogroup A, while compost phylogroups B1, E, F. Diverse STs included ST13 (five isolates), ST258 (four ST86 (one isolate). Within isolates, blaCTX-M-55, blaCTX-M-65, blaCTX-M-15, blaCTX-M-2 genes were identified. also identified blaCMY-2 blaKPC-3 (the latter in carbapenem-susceptible In coli, plasmid-borne mcr-1.1 was across all an IncI2 plasmid. Tigecycline-reduced susceptibility or related missense amino acid substitutions coded marA acrA genes. pneumoiae, blaCTX-M-15 on hand, blaKPC-2 blaKPC-3, other, associated 3GC carbapenem resistance, respectively. allele ~10 kb Tn4401 transposon (tnpR-tnpA-istA-istB-blaKPC-2-tnpA). K sequence data phenotypic analysis linked nonsense substitution mgrB (K3*) marA, acrA, arnB, eptA, pmrB, pmrJ, phoQ colistin resistance. Meanwhile, tigecycline ramR sequence. Additionally, several integron structures, including Int191 (5'CS-dfrA14-3'CS), most prevalent (Int) among study, followed by Int0 (5'CS-3'CS) Int18 (5'CS-dfrA1-3'CS). Conclusions: These results contribute MDR our setting worldwide One Health approach.
Язык: Английский
Процитировано
1
Infection and Drug Resistance, Год журнала: 2024, Номер Volume 17, С. 449 - 462
Опубликована: Фев. 1, 2024
Abstract: Klebsiella pneumoniae ( K. ), a significant contributor to the global challenge of antibiotic resistance, is not only ubiquitous component human microbiome but also potent pathogen capable causing spectrum diseases. This review provides thorough analysis intricate interactions between and immune system, elucidating its substantial impact on metabolic processes. We explore mechanisms employed by evade manipulate responses, including molecular mimicry, modulation, biofilm formation. The further investigates bacterium ' s influence pathways, particularly glycolysis, highlighting how these exacerbate disease severity. emergence multidrug-resistant extremely drug-resistant strains within Enterobacteriaceae family has heightened public health crisis, underscoring urgency for comprehensive research. investigate roles host's complement autophagy, cell death mechanisms, various cytokines in combating infections, shedding light areas that warrant academic investigation. Additionally, discusses challenges posed K1- K2-capsule polysaccharides vaccine development due their complex structures adhesive properties. Acknowledging limited availability effective antimicrobials, this advocates exploring alternative approaches such as immunotherapeutics, vaccinations, phage therapy. consolidate current knowledge , covering classical non-classical subtypes, antimicrobial resistance-mediated genes, virulence factors, epidemiological trends isolation resistance rates. advances our understanding underscores imperative ongoing research collaborative efforts develop new prevention treatment strategies against formidable pathogen. Keywords: pathogenic mechanism, drug-resistant, strategy
Язык: Английский
Процитировано
6
Vaccines, Год журнала: 2024, Номер 12(10), С. 1177 - 1177
Опубликована: Окт. 17, 2024
is well recognized as a serious cause of infection in healthcare-associated settings and immunocompromised individuals; however, accumulating evidence from resource-limited nations documents an alarming rise community-acquired
Язык: Английский
Процитировано
6
Frontiers in Microbiology, Год журнала: 2024, Номер 15
Опубликована: Фев. 7, 2024
Background In recent years, Klebsiella pneumoniae has attracted attention because of its increasing drug resistance. At the same time, migration and pathogenicity caused by virulence genes also bring many difficulties to diagnosis treatment clinical infections. However, it is currently unclear whether there are differences in with changes Objective To understand molecular characteristics expression carbapenem-resistant (CRKP) carbapenem-sensitive (CSKP). Methods Using polymerase chain reaction (PCR), we examined capsule polysaccharide-related 150 isolates CRKP 213 CSKP from local area Ningbo, China. Multilocus sequence typing (MLST) was used analyze phylogenetic relationships isolates. Furthermore, real-time quantitative PCR (RT-qPCR) common CRKP, further verified larval model Galleria mellonella . Results The study found that detection rates rmpA , iroB peg-344 magA aerobactin alls kfu entB were significantly higher compared CRKP. gene types K1 K2 more CSKP, while K5 Hypervirulent (hvKP) predominantly CSKP. strains exhibited noticeable homogeneity, ST11 being predominant type among strains. showed greater diversity ST types, but ST23 still type. Carbapenem-sensitive hypervirulent (CS-hvKP) had rmpA2 (CR-hvKP). wax moth model, survival rate CS-hvKP lower than CR-hvKP. Conclusion There a significant difference distribution between carrying number genes. exhibit Additionally, terms efficiency virulence,
Язык: Английский
Процитировано
5
Antibiotics, Год журнала: 2024, Номер 13(6), С. 561 - 561
Опубликована: Июнь 15, 2024
Klebsiella pneumoniae strains that are resistant to multiple drugs (KPMDRs), which often acquired in hospital settings and lead healthcare-associated infections, pose a serious public health threat, as does hypervirulent K. (hvKp), can also cause infections otherwise healthy individuals. The widespread unnecessary use of antibiotics seen during the recent COVID-19 pandemic has exacerbated challenges posed by antibiotic resistance clinical settings. There is growing concern (hvKp) may acquire genes confer antimicrobial resistance, thus combining an MDR profile with their increased ability spread body sites, causing difficult-to-treat infections. This study aimed compare virulence profiles KPC-3-producing isolates collected over four years (2020–2023). A genome-based surveillance all CRE-K. was used identify genetic differences characterize profiles. Our results provide picture evolution contribute avoiding possible characteristics multi-drug virulence, thought be one main global health, within our hospital.
Язык: Английский
Процитировано
3
Frontiers in Cellular and Infection Microbiology, Год журнала: 2025, Номер 15
Опубликована: Фев. 14, 2025
Background Klebsiella pneumoniae is one of the most important opportunistic pathogens causing healthcare-associated and community-acquired infections worldwide. In recent years, increase in antibiotic resistance caused by hypervirulent K. poses great public health concerns. this study, host-pathogen interactions different strains human animal origins were analyzed microbiological, cell-biological immunological experiments. Methods vitro infection experiments using representatives pathotypes various epithelial macrophage cell lines executed analyzing adhesion, invasion intracellular replication. Experimental conditions involved normoxia hypoxia. Furthermore, survival growth further isolates expressing defined siderophores blood (platelet concentrates, serum) was investigated. All done triplicate statistically significant differences determined. Results Significant adhesion capability, phagocytosis replication measured between pathotypes. Especially, ESBL-producing demonstrated increased host macrophages. A strong cytotoxic effect on intestinal cells observed for . The results from our investigations behavior platelets serum showed that and/or an enlarged capsule are not essential factors proliferation (hypervirulent) components. Conclusion Our revealed new insights into representing pathovars clonal lineages infectious contexts hosts. While a clear limitation study limited strain set used both as potential host, step better understanding pathogenicity its properties stages colonization infection. When developed further, these may offer novel approaches future therapeutics including “anti-virulence strategies”.
Язык: Английский
Процитировано
0
Folia Microbiologica, Год журнала: 2025, Номер unknown
Опубликована: Апрель 8, 2025
Язык: Английский
Процитировано
0
SLAS DISCOVERY, Год журнала: 2025, Номер unknown, С. 100232 - 100232
Опубликована: Апрель 1, 2025
Antimicrobial resistance (AMR) develops into a worldwide health emergency through genetic and biochemical adaptations which enable microorganisms to resist antimicrobial treatment. β-lactamases (blaNDM, blaKPC) efflux pumps (MexAB-OprM) working with mobile elements facilitate fast proliferation of multidrug-resistant (MDR) exttreme drug-resistant (XDR) phenotypes thus creating major concerns for healthcare systems community as well the agricultural sector. The review dissimilarly unifies molecular pathways public implications study epidemiological data monitoring approaches innovative therapeutic solutions. Previous studies separating their attention between genetics clinical outcomes have been combined our approach delivers an all-encompassing analysis AMR. report investigates mechanisms feature enzymatic degradation pump overexpression together target modification horizontal gene transfer because these factors represent important contributors present-day AMR developments. This effects on hospital environments where it affects pathogens including MRSA, carbapenem-resistant Klebsiella pneumoniae, Pseudomonas aeruginosa. document explores modern management methods that comprise WHO GLASS surveillance three strategies such CRISPR-modified genome editing bacteriophage treatments along peptides artificial intelligence diagnostic tools. resolution needs complete scientific global operational alongside state-of-the-art approaches. Worldwide infection burden requires both enhanced prevention procedures next-generation reduce cases effectively.
Язык: Английский
Процитировано
0