A Glycosylphosphatidylinositol-anchored protein from Alternaria alternata triggers cell death and negatively modulates immunity responses in chrysanthemum DOI Creative Commons
Boxiao Dong, Yanyan Sun,

Jing Zhang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 2, 2024

Abstract Chrysanthemum black spot disease is a common fungal caused by A. alternata, which has severely hindered the development of chrysanthemum industry. However, there are few reports on pathogenic factors in especially regarding GPI-APs. In this study, we identified GPI-AP, Aa049, from alternata. Bioinformatics predictions suggest presence GPI anchoring modification site at C-terminus its amino acid sequence, relatively conserved among different Alternaria Nees. Transient overexpression Aa049 Nicotiana benthamiana can induce plant cell death (PCD), and appearance necrosis depends native signal peptide. Compared with wild-type strain, morphology growth rate colony mycelia ΔAa049 deletion mutants did not change. Still integrity wall was damaged, virulence strain significantly reduced, indicating that plays an essential role as factor infection process Furthermore, results quantitative real-time PCR (qRT-PCR) physiological indicators suggested may be exerted through synthesis clearance pathways ROS. This study reveals GPI-APs alternata act to aid pathogen invasion, providing potential target for future biopesticides.

Язык: Английский

Early detection and spectral signature identification of tomato fungal diseases (Alternaria alternata, Alternaria solani, Botrytis cinerea, and Fusarium oxysporum) by RGB and hyperspectral image analysis and machine learning DOI Creative Commons
Seyed Mohamad Javidan, Ahmad Banakar, Keyvan Asefpour Vakilian

и другие.

Heliyon, Год журнала: 2024, Номер 10(19), С. e38017 - e38017

Опубликована: Сен. 19, 2024

Язык: Английский

Процитировано

6
A glycosylphosphatidylinositol-anchored protein from Alternaria alternata triggers cell death and negatively modulates immunity responses in chrysanthemum DOI
Boxiao Dong, Yanyan Sun,

Jing Zhang

и другие.

Plant Cell Reports, Год журнала: 2024, Номер 43(12)

Опубликована: Ноя. 18, 2024

Язык: Английский

Процитировано

1
Unveiling the Full Protein Effectorome of the Black Sigatoka Pathogen Pseudocercospora fijiensis—An In Silico Approach DOI Creative Commons
Karla Gisel Carreón-Anguiano, Jewel Nicole Anna Todd, César De los Santos-Briones

и другие.

Microbiology Research, Год журнала: 2024, Номер 15(3), С. 1880 - 1899

Опубликована: Сен. 14, 2024

Pseudocercospora (previously Mycosphaerella) fijiensis is a hemibiotroph fungus and the causal agent of black Sigatoka disease, one most significant threats to banana production worldwide. Only few genomics reports have paid any attention effector proteins, which are key players in pathogenicity. These focus on canonical effectors: small secreted rich cysteines, containing signal peptide no transmembrane domain. Thus, bias previous has resulted non-canonical effectors being, effect, excluded from discussion P. Here, using WideEffHunter EffHunter, bioinformatic tools identify effectors, respectively, we predict, for first time, full effectorome fijiensis. This complete comprises 5179 proteins: 240 4939 effectors. Protein families related functions hemibiotrophic lifestyle, such as Salicylate hydroxylase Isochorismatase, widely represented genome. An analysis gene distribution core dispensable scaffolds both classes revealed novel genomic structure effectorome. The majority (canonical non-canonical) were found be harbored scaffolds, while less than 10% all non-canonical. Additionally, motifs RXLR, YFWxC, LysM, EAR, [Li]xAR, PDI, CRN, ToxA (more enriched genome), well occurrence also observed four other fungi, evidences that these phenomena not unique fijiensis; rather, they occurring characteristics fungi.

Язык: Английский

Процитировано

0
Fungi associated with potato and their significance as pathogens in Russia DOI Creative Commons
Philipp B. Gannibal,

E. V. Poluektova,

T. Yu. Gagkaeva

и другие.

Plant Protection News, Год журнала: 2024, Номер 107(4), С. 148 - 164

Опубликована: Дек. 23, 2024

Despite the great attention paid to study of potato diseases, inaccuracies in names their practical significance, and incorrect use scientific causal agents can be commonly found literature. This may lead confusion reduces value published information phytosanitary efforts. review continues series started with publications devoted wheat barley diseases. article includes about main diseases pathogenic organisms causing them, as well species emerging a potential threat potato. The current taxonomic status fungal fungus-like associated various organs is given, breadth distribution degree impact on crop are summarized. micromycetes were divided into two groups according importance. first group represented by fungi importance pathogens 13 economically important second consists 25 minor poorly studied unconfirmed harmfulness, or endophytic fungi. Perceptions ability cause disease remain controversial available data require confirmation. dataset used reference for more accurate description situation. will also helpful targeted studies using molecular techniques clarify taxonomy geographic ranges provide detailed levels this crop.

Язык: Английский

Процитировано

0
A Glycosylphosphatidylinositol-anchored protein from Alternaria alternata triggers cell death and negatively modulates immunity responses in chrysanthemum DOI Creative Commons
Boxiao Dong, Yanyan Sun,

Jing Zhang

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Сен. 2, 2024

Abstract Chrysanthemum black spot disease is a common fungal caused by A. alternata, which has severely hindered the development of chrysanthemum industry. However, there are few reports on pathogenic factors in especially regarding GPI-APs. In this study, we identified GPI-AP, Aa049, from alternata. Bioinformatics predictions suggest presence GPI anchoring modification site at C-terminus its amino acid sequence, relatively conserved among different Alternaria Nees. Transient overexpression Aa049 Nicotiana benthamiana can induce plant cell death (PCD), and appearance necrosis depends native signal peptide. Compared with wild-type strain, morphology growth rate colony mycelia ΔAa049 deletion mutants did not change. Still integrity wall was damaged, virulence strain significantly reduced, indicating that plays an essential role as factor infection process Furthermore, results quantitative real-time PCR (qRT-PCR) physiological indicators suggested may be exerted through synthesis clearance pathways ROS. This study reveals GPI-APs alternata act to aid pathogen invasion, providing potential target for future biopesticides.

Язык: Английский

Процитировано

0