A Glycosylphosphatidylinositol-anchored protein from Alternaria alternata triggers cell death and negatively modulates immunity responses in chrysanthemum DOI Creative Commons
Boxiao Dong, Yanyan Sun,

Jing Zhang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 2, 2024

Abstract Chrysanthemum black spot disease is a common fungal caused by A. alternata, which has severely hindered the development of chrysanthemum industry. However, there are few reports on pathogenic factors in especially regarding GPI-APs. In this study, we identified GPI-AP, Aa049, from alternata. Bioinformatics predictions suggest presence GPI anchoring modification site at C-terminus its amino acid sequence, relatively conserved among different Alternaria Nees. Transient overexpression Aa049 Nicotiana benthamiana can induce plant cell death (PCD), and appearance necrosis depends native signal peptide. Compared with wild-type strain, morphology growth rate colony mycelia ΔAa049 deletion mutants did not change. Still integrity wall was damaged, virulence strain significantly reduced, indicating that plays an essential role as factor infection process Furthermore, results quantitative real-time PCR (qRT-PCR) physiological indicators suggested may be exerted through synthesis clearance pathways ROS. This study reveals GPI-APs alternata act to aid pathogen invasion, providing potential target for future biopesticides.

Language: Английский

Early detection and spectral signature identification of tomato fungal diseases (Alternaria alternata, Alternaria solani, Botrytis cinerea, and Fusarium oxysporum) by RGB and hyperspectral image analysis and machine learning DOI Creative Commons
Seyed Mohamad Javidan, Ahmad Banakar, Keyvan Asefpour Vakilian

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(19), P. e38017 - e38017

Published: Sept. 19, 2024

Language: Английский

Citations

6
A glycosylphosphatidylinositol-anchored protein from Alternaria alternata triggers cell death and negatively modulates immunity responses in chrysanthemum DOI
Boxiao Dong, Yanyan Sun,

Jing Zhang

et al.

Plant Cell Reports, Journal Year: 2024, Volume and Issue: 43(12)

Published: Nov. 18, 2024

Language: Английский

Citations

1
Unveiling the Full Protein Effectorome of the Black Sigatoka Pathogen Pseudocercospora fijiensis—An In Silico Approach DOI Creative Commons
Karla Gisel Carreón-Anguiano, Jewel Nicole Anna Todd, César De los Santos-Briones

et al.

Microbiology Research, Journal Year: 2024, Volume and Issue: 15(3), P. 1880 - 1899

Published: Sept. 14, 2024

Pseudocercospora (previously Mycosphaerella) fijiensis is a hemibiotroph fungus and the causal agent of black Sigatoka disease, one most significant threats to banana production worldwide. Only few genomics reports have paid any attention effector proteins, which are key players in pathogenicity. These focus on canonical effectors: small secreted rich cysteines, containing signal peptide no transmembrane domain. Thus, bias previous has resulted non-canonical effectors being, effect, excluded from discussion P. Here, using WideEffHunter EffHunter, bioinformatic tools identify effectors, respectively, we predict, for first time, full effectorome fijiensis. This complete comprises 5179 proteins: 240 4939 effectors. Protein families related functions hemibiotrophic lifestyle, such as Salicylate hydroxylase Isochorismatase, widely represented genome. An analysis gene distribution core dispensable scaffolds both classes revealed novel genomic structure effectorome. The majority (canonical non-canonical) were found be harbored scaffolds, while less than 10% all non-canonical. Additionally, motifs RXLR, YFWxC, LysM, EAR, [Li]xAR, PDI, CRN, ToxA (more enriched genome), well occurrence also observed four other fungi, evidences that these phenomena not unique fijiensis; rather, they occurring characteristics fungi.

Language: Английский

Citations

0
Fungi associated with potato and their significance as pathogens in Russia DOI Creative Commons
Philipp B. Gannibal,

E. V. Poluektova,

T. Yu. Gagkaeva

et al.

Plant Protection News, Journal Year: 2024, Volume and Issue: 107(4), P. 148 - 164

Published: Dec. 23, 2024

Despite the great attention paid to study of potato diseases, inaccuracies in names their practical significance, and incorrect use scientific causal agents can be commonly found literature. This may lead confusion reduces value published information phytosanitary efforts. review continues series started with publications devoted wheat barley diseases. article includes about main diseases pathogenic organisms causing them, as well species emerging a potential threat potato. The current taxonomic status fungal fungus-like associated various organs is given, breadth distribution degree impact on crop are summarized. micromycetes were divided into two groups according importance. first group represented by fungi importance pathogens 13 economically important second consists 25 minor poorly studied unconfirmed harmfulness, or endophytic fungi. Perceptions ability cause disease remain controversial available data require confirmation. dataset used reference for more accurate description situation. will also helpful targeted studies using molecular techniques clarify taxonomy geographic ranges provide detailed levels this crop.

Language: Английский

Citations

0
A Glycosylphosphatidylinositol-anchored protein from Alternaria alternata triggers cell death and negatively modulates immunity responses in chrysanthemum DOI Creative Commons
Boxiao Dong, Yanyan Sun,

Jing Zhang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 2, 2024

Abstract Chrysanthemum black spot disease is a common fungal caused by A. alternata, which has severely hindered the development of chrysanthemum industry. However, there are few reports on pathogenic factors in especially regarding GPI-APs. In this study, we identified GPI-AP, Aa049, from alternata. Bioinformatics predictions suggest presence GPI anchoring modification site at C-terminus its amino acid sequence, relatively conserved among different Alternaria Nees. Transient overexpression Aa049 Nicotiana benthamiana can induce plant cell death (PCD), and appearance necrosis depends native signal peptide. Compared with wild-type strain, morphology growth rate colony mycelia ΔAa049 deletion mutants did not change. Still integrity wall was damaged, virulence strain significantly reduced, indicating that plays an essential role as factor infection process Furthermore, results quantitative real-time PCR (qRT-PCR) physiological indicators suggested may be exerted through synthesis clearance pathways ROS. This study reveals GPI-APs alternata act to aid pathogen invasion, providing potential target for future biopesticides.

Language: Английский

Citations

0