Journal of Economic Entomology, Год журнала: 2024, Номер unknown
Опубликована: Ноя. 4, 2024
Abstract The endoparasitoid Asecodes hispinarum (Bouček) (Hymenoptera: Eulophidae) serves as an effective biological control agent against Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae), a notorious palm pest. Endosymbionts found in parasitoids and their hosts have attracted significant attention due to substantial influence on biocontrol efficacy. In this study, we employed 16S rRNA sequencing, polymerase chain reaction, fluorescence situ hybridization assess the symbiotic bacteria composition, diversity, phylogeny, localization A. its host B. longissima. Our findings showed differences richness, composition of among different life stages Notably, bacterial was similar that Firmicutes Proteobacteria were dominant phyla, while Wolbachia genera across parasitoid host. It discovered for first time present with high infection rate at ≥ 96.67%. strain placed supergroup A, whereas it categorized under B Furthermore, is concentrated abdomen hispinarum, particularly levels observed ovipositors female adults. These highlight diversity both longissima, providing foundation development population regulation strategies targeting
Язык: Английский