Novel silver nanoparticle-based biomaterials for combating Klebsiella pneumoniae biofilms
Frontiers in Microbiology,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 9, 2025
Klebsiella
pneumoniae
is
a
significant
nosocomial
pathogen
that
has
developed
resistance
to
multiple
antibiotics,
often
forming
biofilms
enhance
its
virulence.
This
study
investigated
the
efficacy
of
novel
nanoformulation,
AgNPs@chitosan-NaF,
in
combating
K.
biofilms.
Antimicrobial
susceptibility
testing
was
performed
assess
antibiotic
profile
isolates.
The
antibiofilm
activity
AgNPs@chitosan-NaF
evaluated
using
crystal
violet
staining
and
scanning
electron
microscopy.
underlying
mechanisms
action
were
through
gene
expression
analysis.
majority
isolates
exhibited
high
levels
multidrug
resistance.
demonstrated
superior
biofilm
inhibition
compared
AgNPs@chitosan,
significantly
reducing
biomass
disrupting
architecture
at
MICs
ranging
from
0.125
1
μg/mL.
Mechanistic
studies
revealed
nanoformulation
downregulated
key
biofilm-associated
genes,
including
treC,
fimA,
mrkA,
ecpA.
While
concentration-dependent
cytotoxic
effect
on
both
normal
cancer
cell
lines,
minimal
cytotoxicity
observed
concentrations
below
31.25
highlights
synergistic
silver
nanoparticles,
chitosan,
sodium
fluoride
emerges
as
promising
therapeutic
strategy
address
challenge
multidrug-resistant
bacterial
infections.
Язык: Английский
Virulence factors of Klebsiella pneumoniae: Insights into canonical and emerging mechanisms driving pathogenicity and drug resistance
The Microbe,
Год журнала:
2025,
Номер
unknown, С. 100289 - 100289
Опубликована: Март 1, 2025
Язык: Английский
Zooplankton biomass as a promising new agent for biomedical applications
Biocatalysis and Agricultural Biotechnology,
Год журнала:
2025,
Номер
unknown, С. 103560 - 103560
Опубликована: Март 1, 2025
Язык: Английский
Natural Inhibitors of Salmonella MDR Efflux Pumps AcrAB and AcrD: An Integrated In Silico, Molecular, and In Vitro Investigation
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(23), С. 12949 - 12949
Опубликована: Дек. 2, 2024
Multidrug-resistant
(MDR)
Язык: Английский