Interactive effects of elevated temperature and Photobacterium swingsii infection on the survival and immune response of marine mussels (Perna canaliculus): A summer mortality scenario

Marine Environmental Research, Год журнала: 2024, Номер 196, С. 106392 - 106392

Опубликована: Фев. 3, 2024

The New Zealand Greenshell™ mussel (Perna canaliculus) is an economically important aquaculture species. Prolonged increases in seawater temperature above thermotolerance ranges pose a significant threat to survival and health, potentially increasing susceptibility bacterial infections. Using challenge experiments, this study examined the combined effects of increased (Photobacterium swingsii) infection on animal survival, haemocyte biochemical responses adult mussels. Mussels maintained at three temperatures (16, 20 24 °C) for seven days were either not injected (control), with sterile marine broth (injection control) or P. swingsii (challenged medium high doses) monitored daily five days. Haemolymph tissue samples collected 24, 48, 72, 96, 120 h post-challenge analysed quantify colonies, responses. infected exhibited mortalities °C, likely due compromised immune system, but no observed when was only stressor. Bacterial colony counts haemolymph decreased over time, suggesting clearance followed by activation signalling pathways. Total viability data supports defence functions being stimulated presence pathogen loads °C. In gill tissue, oxidative stress responses, measured as total antioxidant capacity malondialdehyde (MDA) levels, higher mussels (compared controls) after 24h 120h lowest (16 highest (24 °C), indicating stressors. Overall, work confirms that Photobacterium pathogenic canaliculus indicates may be more vulnerable pathogens under conditions elevated temperature, such those predicted future climate change scenarios.

Язык: Английский

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Quercetin Alleviates the Progression of Breast Cancer-Related Depression via Inhibiting the Pyroptosis and Promoting the Immune Response

Mediators of Inflammation, Год журнала: 2022, Номер 2022, С. 1 - 14

Опубликована: Март 3, 2022

Background. Breast cancer-related depression (BCRD) seriously inhibits the life quality of patients with breast cancer. The Xiaoyao Kangai Jieyu Formula is known to inhibit progression depression. However, detailed function in BCRD remains unclear. Methods. Network pharmacology was constructed assess downstream target BCRD. In addition, tail suspension test, sucrose preference and forced swimming test were used symptom mice. Fluoro-Jade B staining performed observe structure neurons. RT-qPCR western blot applied evaluate mRNA protein levels. Besides, ELISA inflammatory responses immune response-related cytokines. Results. Quercetin identified as key component Formula. significantly inhibited BCRD-induced neuron pyroptosis via downregulation PYD card domain containing (ASC), NLR family pyrin 3 (NLRP3), caspase-1, quercetin could reverse BCRD-caused inhibition viability. attenuated mice, it contents 5-hydroxytryptamine (5-HT), dopamine (DA), neutrophil elastase (NE) Moreover, promote xenograft mice upregulation interleukin- (IL-) 2, interferon-γ (IFN-γ), IL-10. Conclusion. Quercetin, active ingredient Formula, effectively mitigated by inhibiting pyroptosis, promoting response, improving serum metabolism.

Язык: Английский

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Ocean acidificationf affects the bioenergetics of marine mussels as revealed by high-coverage quantitative metabolomics

The Science of The Total Environment, Год журнала: 2022, Номер 858, С. 160090 - 160090

Опубликована: Ноя. 13, 2022

Язык: Английский

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Exploring the mechanisms behind swimming performance limits to ocean warming and acidification in the Atlantic king scallop, Pecten maximus

Frontiers in Ecology and Evolution, Год журнала: 2024, Номер 12

Опубликована: Апрель 9, 2024

Recently, we could show that scallops limitations of muscular performance like a reduced force under ocean warming and acidification. However, the underlying mechanisms at cellular level are not completely understood. Metabolomics has become valuable tool to evaluate responses marine organisms various stressors. In present study therefore used semi-targeted, multi tissue NMR based metabolomic approach analyze metabolite patterns in Atlantic king scallop, Pecten maximus , were long-term acclimated different end century conditions (OW), acidification (OA) their combination (OWA). We investigated specific metabolic profiles concentrations frozen tissues from gills, mantle phasic tonic adductor muscle P. using 1 H-HR-MAS spectroscopy. A set 33 metabolites revealed clear tissue-specific pattern which can be attributed individual functions respective type. then evaluated impact OW, OA OWA on tissues. OW was main driver changes metabolites. particular, energy-related seem play an important role physiological response OWA. with pathway analysis network exploration propose possible correlation between limited swimming future climate. While seems mainly depend net consumption energy related such as ATP phospho-L-arginine, rely metabolizing amino acids beta-oxidation account for elevated energetic requirements

Язык: Английский

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Spawning acts as a metabolic stressor enhanced by hypoxia and independent of sex in a broadcast marine spawner

The Science of The Total Environment, Год журнала: 2023, Номер 909, С. 168419 - 168419

Опубликована: Ноя. 17, 2023

Broadcast spawners, like the blue mussel Mytilus edulis, experience substantial energy expenditure during spawning due to extensive gamete release that can divert from other functions. This energetic cost might be intensified by environmental stressors, including hypoxia suppress aerobic metabolism. However, implications of in marine broadcast spawners have not been well studied. We examined effects short-term (7 days) and on mitochondrial activity, reactive oxygen species (ROS) production, cellular allocation (ratio tissue reserves demand) somatic tissues M. edulis. Under normoxic conditions, post-spawning (72 h) recovery correlated with increased phosphorylation (OXPHOS) rate mitochondria digestive gland, while inhibited this response. Regardless levels, ROS production decreased after spawning, indicating edulis' ability prevent oxidative stress. Spawning led reduced (the gills gland), highlighting significant primarily fueled lipid protein breakdown. Additionally, dropped 3 h post-spawning, a shift demand supply. Normoxic conditions allowed 72 h, but hindered recuperation. These findings underscore spawning's bioenergetic challenge for potentially elevating mortality risk, especially hypoxic coastal habitats.

Язык: Английский

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Biomarkers of mussel exposure to Vibrionaceae: A review

Aquaculture International, Год журнала: 2024, Номер 32(6), С. 7595 - 7627

Опубликована: Май 10, 2024

Abstract For more than a century, bacterial infections caused by Vibrio spp. have affected various species of mussels around the world, with limited insights into responses implemented against these infections. A combination chemical analyses and carefully selected biological endpoints from haemolymph tissues can be used to identify welfare status potentially protect aquatic ecosystems catastrophic health threats. Recent developments in biomarker identification tools, such as omics bioinformatics, been successfully applied evaluate effect environmental pollutants other chemicals on mussels. However, application biomarkers assess mussel is limited. This review describes available scientific literature research for -mussel interactions, those aspects related disease assessment, grouped exposure, effects, susceptibility. From review, it clear that when integrated are used, they provide deeper understanding relative potential susceptibility better management practices. Furthermore, data build resilience climate change conditions, strengthen biosecurity programs, improve farming processing efficiency, add value terms market-desirable traits. These hold promise advancing sustainability efforts within aquaculture industry. Graphical abstract

Язык: Английский

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Exploring the immune resilience of Mediterranean mussels: recent advances and future directions

Fish & Shellfish Immunology, Год журнала: 2025, Номер 158, С. 110147 - 110147

Опубликована: Янв. 19, 2025

Язык: Английский

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Molecular responses of Mytilus coruscus hemocytes to lipopolysaccharide and peptidoglycan as revealed by 4D-DIA based quantitative proteomics analysis

Fish & Shellfish Immunology, Год журнала: 2025, Номер 158, С. 110143 - 110143

Опубликована: Янв. 20, 2025

Язык: Английский

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Physiological costs of infection by the invasive parasitic copepod Mytilicola intestinalis accumulate across temporal scales in the blue mussel Mytilus edulis

Journal of Invertebrate Pathology, Год журнала: 2025, Номер 212, С. 108374 - 108374

Опубликована: Май 27, 2025

Язык: Английский

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Gender-Specific Metabolic Responses of Crassostrea hongkongensis to Infection with Vibrio harveyi and Lipopolysaccharide

Antioxidants, Год журнала: 2022, Номер 11(6), С. 1178 - 1178

Опубликована: Июнь 15, 2022

Gender differences in the hemocyte immune response of Hong Kong oyster Crassostrea hongkongensis to Vibrio harveyi and lipopolysaccharide (LPS) infection exist. To determine if a gender difference also exists, we use 1H NMR-based metabolomics method investigate responses C. hepatopancreas tissues V. LPS infection. Both infections induced pronounced gender- immune-specific metabolic tissues. Responses are mainly presented changes substances involved energy metabolism (decreased glucose, ATP, AMP males increased ATP LPS-infected females), oxidative stress glutathione decreased tryptophan phenylalanine choline proline tricarboxylic acid (TCA) cycle α-ketoglutarate fumarate males, osmotic regulation trigonelline taurine harveyi-infected betaine females). Results suggest that post-spawning-phase male oysters have more significant greater ability cope with than female oysters. We propose impact should be taken into consideration aftermath farming or disease natural seas.

Язык: Английский

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