Bioactive lipids in the skin barrier mediate its functionality in health and disease DOI Creative Commons
Anna Nicolaou, Alexandra C. Kendall

Pharmacology & Therapeutics, Год журнала: 2024, Номер 260, С. 108681 - 108681

Опубликована: Июнь 17, 2024

Our skin protects us from external threats including ultraviolet radiation, pathogens and chemicals, prevents excessive trans-epidermal water loss. These varied activities are reliant on a vast array of lipids, many which unique to skin, that support physical, microbiological immunological barriers. The cutaneous physical barrier is dependent specific lipid matrix surrounds terminally-differentiated keratinocytes in the stratum corneum. Sebum- keratinocyte-derived lipids cover skin's surface regulate microbiota. Meanwhile, signal between resident infiltrating immune cells, driving inflammation its resolution response other threats. Lipids particular importance include ceramides, crucial for corneum formation therefore functionality, fatty acids, contribute acidic pH microbiota, as well matrix, bioactive metabolites these involved cell signalling, inflammation, numerous processes. diverse complex maintain homeostasis healthy implicated diseases, unrelated systemic conditions with manifestations, processes such ageing. also gut-skin axis, signalling two sites. Therefore, provide valuable resource exploration processes, local disease development progression, accessible biomarker discovery disease, an opportunity fully understand relationship host Investigation could diagnostic prognostic biomarkers, help identify new targets interventions. Development improvement existing vitro silico approaches explore lipidome, advances lipidomics technologies, will facilitate ongoing progress research.

Язык: Английский

Brucea javanica derived exosome-like nanovesicles deliver miRNAs for cancer therapy DOI
Ge Yan,

Qiyao Xiao,

Jingyu Zhao

и другие.

Journal of Controlled Release, Год журнала: 2024, Номер 367, С. 425 - 440

Опубликована: Фев. 2, 2024

Язык: Английский

Процитировано

48
Biogenesis and delivery of extracellular vesicles: harnessing the power of EVs for diagnostics and therapeutics DOI Creative Commons

Jivin Yu,

Saba Sane, Ji-Eun Kim

и другие.

Frontiers in Molecular Biosciences, Год журнала: 2024, Номер 10

Опубликована: Янв. 3, 2024

Extracellular vesicles (EVs) are membrane-enclosed particles secreted by a variety of cell types. These encapsulate diverse range molecules, including proteins, nucleic acids, lipids, metabolites, and even organelles derived from their parental cells. While EVs have emerged as crucial mediators intercellular communication, they also hold immense potential both biomarkers therapeutic agents for numerous diseases. A thorough understanding EV biogenesis is the development EV-based diagnostic developments since composition can reflect health disease status donor cell. Moreover, when taken up target cells, exert profound effects on gene expression, signaling pathways, cellular behavior, which makes these biomolecules enticing targets interventions. Yet, despite decades research, intricate processes underlying cells subsequent uptake recipient remain poorly understood. In this review, we aim to summarize current insights advancements in mechanisms EVs. By shedding light fundamental governing delivery, review underscores basic mechanistic research pave way developing novel strategies applications.

Язык: Английский

Процитировано

28
Extracellular Vesicle Preparation and Analysis: A State‐of‐the‐Art Review DOI Creative Commons
Zesheng Wang, Xiaoyu Zhou, Qinglong Kong

и другие.

Advanced Science, Год журнала: 2024, Номер 11(30)

Опубликована: Июнь 14, 2024

Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.

Язык: Английский

Процитировано

27
Leveraging nature’s nanocarriers: Translating insights from extracellular vesicles to biomimetic synthetic vesicles for biomedical applications DOI Creative Commons
Yunxi Chen,

Noélie Douanne,

T.-C. Wu

и другие.

Science Advances, Год журнала: 2025, Номер 11(9)

Опубликована: Фев. 26, 2025

Naturally occurring extracellular vesicles (EVs) and synthetic nanoparticles like liposomes have revolutionized precision diagnostics medicine. EVs excel in biocompatibility cell targeting, while offer enhanced drug loading capacity scalability. The clinical translation of is hindered by challenges including low yield heterogeneity, whereas face rapid immune clearance limited targeting efficiency. To bridge these gaps, biomimetic (SVs) emerged as innovative platforms, combining the advantageous properties liposomes. This review emphasizes critical aspects EV biology, such mechanisms EV-cell interaction source-dependent functionalities modulation, tissue regeneration, informing SV engineering. We reviewed a broad array SVs, with focus on lipid bilayered functionalized proteins. These include cell-derived nanovesicles, protein-functionalized liposomes, hybrid vesicles. By addressing current highlighting opportunities, this aims to advance SVs for transformative biomedical applications.

Язык: Английский

Процитировано

2
Role of lipid signalling in extracellular vesicles-mediated cell-to-cell communication DOI Creative Commons
Jordan Fyfe,

Ilaria Casari,

Marcello Manfredi

и другие.

Cytokine & Growth Factor Reviews, Год журнала: 2023, Номер 73, С. 20 - 26

Опубликована: Авг. 23, 2023

Lipid signalling plays a crucial role in extracellular vesicle (EV)-mediated cell-to-cell communication. Extracellular vesicles are small membrane-bound structures released by various cell types into the environment. They include exosomes, microvesicles, and apoptotic bodies. These contain variety of bioactive molecules, including proteins, nucleic acids (such as miRNAs mRNAs), lipids. Lipids important components EVs involved aspects their biogenesis, cargo sorting, functional effects on target cells. In this review, we will discuss how lipid is EV-mediated summary, intricately vesicle-mediated The composition influences interactions with cells, recipient Understanding lipids communication essential for deciphering mechanisms underlying intercellular developing potential therapeutic strategies based EVs.

Язык: Английский

Процитировано

30
Environmental pollutants and exosomes: A new paradigm in environmental health and disease DOI
Afsaneh Javdani-Mallak,

Iman Salahshoori

The Science of The Total Environment, Год журнала: 2024, Номер 925, С. 171774 - 171774

Опубликована: Март 18, 2024

Язык: Английский

Процитировано

14
Insight into the Functional Dynamics and Challenges of Exosomes in Pharmaceutical Innovation and Precision Medicine DOI Creative Commons
Anu Sharma, Anita Yadav,

Aparajita Nandy

и другие.

Pharmaceutics, Год журнала: 2024, Номер 16(6), С. 709 - 709

Опубликована: Май 24, 2024

Of all the numerous nanosized extracellular vesicles released by a cell, endosomal-originated exosomes are increasingly recognized as potential therapeutics, owing to their inherent stability, low immunogenicity, and targeted delivery capabilities. This review critically evaluates transformative of exosome-based modalities across pharmaceutical precision medicine landscapes. Because precise biomolecular cargo delivery, posited ideal candidates in drug enhancing regenerative strategies, advancing diagnostic technologies. Despite significant market growth projections exosome therapy, its utilization is encumbered substantial scientific regulatory challenges. These include lack universally accepted protocols for isolation complexities associated with navigating environment, particularly guidelines set forth U.S. Food Drug Administration (FDA). presents comprehensive overview current research trajectories aimed at addressing these impediments discusses prospective advancements that could substantiate clinical translation exosomal therapies. By providing analysis both capabilities hurdles therapeutic applications, this article aims inform direct future paradigms, thereby fostering integration systems into mainstream practice.

Язык: Английский

Процитировано

14
Unseen Weapons: Bacterial Extracellular Vesicles and the Spread of Antibiotic Resistance in Aquatic Environments DOI Open Access
Muttiah Barathan, Sook Luan Ng, Yogeswaran Lokanathan

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(6), С. 3080 - 3080

Опубликована: Март 7, 2024

This paper sheds light on the alarming issue of antibiotic resistance (ABR) in aquatic environments, exploring its detrimental effects ecosystems and public health. It examines multifaceted role use aquaculture, agricultural runoff, industrial waste fostering development dissemination resistant bacteria. The intricate interplay between various environmental factors, horizontal gene transfer, bacterial extracellular vesicles (BEVs) accelerating spread ABR is comprehensively discussed. Various BEVs carrying genes like blaCTX-M, tetA, floR, sul/I, as well their contribution to dominance multidrug-resistant bacteria, are highlighted. potential both a threat tool combating explored, with promising strategies targeted antimicrobial delivery systems probiotic-derived EVs holding significant promise. underscores urgency understanding environments. By unraveling these unseen weapons, we pave way for developing effective mitigate ABR, advocating multidisciplinary approach that includes stringent regulations, enhanced wastewater treatment, adoption sustainable practices aquaculture.

Язык: Английский

Процитировано

12
Vascular Endothelial Cell-Derived Exosomal Sphingosylphosphorylcholine Attenuates Myocardial Ischemia–Reperfusion Injury through NR4A2-Mediated Mitophagy DOI Open Access
Yifan Yu, Zhiliang Li, Yuqing Cai

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(6), С. 3305 - 3305

Опубликована: Март 14, 2024

Cardiomyocyte survival is a critical contributing process of host adaptive responses to cardiovascular diseases (CVD). Cells the endothelium have recently been reported promote cardiomyocyte through exosome-loading cargos. Sphingosylphosphorylcholine (SPC), an intermediate metabolite sphingolipids, mediates protection against myocardial infarction (MI). Nevertheless, mechanism SPC delivery by vascular endothelial cell (VEC)-derived exosomes (VEC-Exos) remains uncharacterized at time this writing. The present study utilized mice model ischemia/reperfusion (I/R) demonstrate that administration via tail vein injection significantly diminished severity I/R-induced cardiac damage and prevented apoptosis cardiomyocytes. Moreover, was here identified as primary mediator observed protective effects VEC-Exos. In addition, within investigation, in vitro experiments using cardiomyocytes showed counteracted I/R injury activating Parkin nuclear receptor subfamily group A member 2/optineurin (NR4A2/OPTN) pathways, turn resulting increased levels mitophagy I/R-affected myocardium. highlights potential therapeutic SPC-rich secreted VECs on alleviating cardiomyocytes, thereby providing strong experimental evidence support application target prevention treatment infarction.

Язык: Английский

Процитировано

9
Engineered and Mimicked Extracellular Nanovesicles for Therapeutic Delivery DOI Creative Commons
Véréna Poinsot, Nathalie Pizzinat, Varravaddheay Ong‐Meang

и другие.

Nanomaterials, Год журнала: 2024, Номер 14(7), С. 639 - 639

Опубликована: Апрель 6, 2024

Exosomes are spherical extracellular nanovesicles with an endosomal origin and unilamellar lipid-bilayer structure sizes ranging from 30 to 100 nm. They contain a large range of proteins, lipids, nucleic acid species, depending on the state vesicle (EV)-secreting cell. EVs’ function is encapsulate part EV-producing cell content, transport it through biological fluids targeted recipient, deliver their cargos specifically within aimed recipient cells. Therefore, exosomes considered be potential drug-delivery systems that can stably cargo into Various cell-derived produced for medical issues, but use therapeutic purposes still faces several problems. Some these difficulties avoided by resorting hemisynthetic approaches. We highlight here uses alternative exosome-mimes involving cell-membrane coatings artificial nanocarriers or hybridization between liposomes. also detail drug-loading strategies deployed make them drug-carrier summarize ongoing clinical trials exosome-like structures. Finally, we open questions before considering disposals confident delivery.

Язык: Английский

Процитировано

9