Digital Chinese Medicine,
Год журнала:
2023,
Номер
6(3), С. 295 - 306
Опубликована: Сен. 1, 2023
The
present
study
was
aimed
to
investigate
the
neuroprotective
effect
of
Croton
hirtus
(CH)
extract
against
streptozotocin
(STZ)
in
rats.
(i)
sub-chronic
toxicity
consisted
24
adult
rats
either
sex
weighing
from
160
200
g
were
divided
into
four
groups
with
six
each
group.
Rats
group
1
received
Dimethyl
sulfoxide
(DMSO)
mixed
saline;
2,
3,
and
4
100,
200,
400
mg/kg
methanolic
CH
(MECH)
orally
by
gavage
administration
for
28
d,
respectively.
functional
observation
battery
locomotor
activity
graded
as
part
their
neurobehavioral
brain
regions,
including
cortex
hippocampus,
analyzed
neuropathological
abnormalities.
(ii)
main
research
30
male
Wistar
g,
which
five
group,
control
(I),
STZ
(II),
Donepezil
(III),
MECH
(100
mg/kg,
IV),
(200
V)
groups.
I
citrate
buffer
DMSO
saline
14
d.
II
via
intracerebroventricular
injection
(3
bilateral
ICV-STZ)
on
days
3
followed
III,
IV,
V
[3
mg/(kg·d),
p.o.]
[100
tested
learning
memory
parameters
such
Morris
water
maze
(MWM)
passive
avoidance
test
(PAT).
They
sacrificed
after
completing
behavioural
experiments;
brains
harvested
estimate
acetylcholinesterase
(AChE),
lipid
peroxidation
(LPO),
glutathione
(GSH),
superoxide
dismutase
(SOD)
using
UV-Visible
Spectrophotometer;
caspase-3
evaluated
total
fluorescence
emission
spectra;
amyloid
β
(Aβ)
levels
detected
enzyme-linked
immuosorbent
assay
(ELISA);
histopathological
examination
conducted
CA1
region
hippocampus.
results
revealed
that
open
field
line
crossing,
rearing,
entering
middle
square,
defecating,
or
urinating
did
not
differ
significantly
(P
>
0.05).
show
any
lesions
neuronal
cells
inflammation
both
regions
compared
findings
demonstrated
STZ-treated
showed
a
significant
increase
impairment
<
0.001),
AChE,
caspase-3,
Aβ
(1-40
1-42),
LPO
increased
decrease
found
SOD
0.001)
GSH
0.01).
Moreover,
damage
In
contrast,
STZ-induced
biochemical
impairments
considerably
decreased
treatment
dose-dependently.
prevented
deficit
induced
due
antioxidant
action.
Restoration
cholinergic
functioning
may
be
cause
improvement.
Therefore,
able
treat
cognitive
disorders
like
Alzheimer's
disease
(AD).
Drug Design Development and Therapy,
Год журнала:
2024,
Номер
Volume 18, С. 4565 - 4584
Опубликована: Окт. 1, 2024
Depression,
a
leading
cause
of
disability
worldwide,
is
characterized
by
dysfunction
immature
neurons,
resulting
in
dysregulated
calcium
homeostasis
and
impaired
structural
plasticity.
Jujuboside
A
(JuA),
biologically
active
compound
derived
from
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(21), С. 11796 - 11796
Опубликована: Ноя. 2, 2024
This
experimental
study
was
performed
to
evaluate
the
alterations
in
expression
of
a
few
subunits
composing
glutamate
AMPA
(a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic
acid)
and
NMDA
(N-methyl-D-aspartate)
receptors
hippocampal
cells
Wistar
rats
response
long-term
fluoride
(F-)
exposure.
The
animals
were
given
water
with
background
0.4
(control),
5,
20,
50
ppm
F-
(as
NaF)
for
12
months.
cognitive
capacities
examined
by
novel
object
recognition
(NOR),
Y-maze
test,
Morris
maze
tests.
RT-qPCR
Western
blotting
techniques
used
different
at
transcriptional
translational
levels,
respectively.
Long-term
poisoning
disturbed
formation
hippocampus-dependent
working
spatial
non-spatial
memory.
Gria1,
Gria2,
Gria3
genes
encoding
comparable
hippocampi
control
F--exposed
animals,
although
levels
both
Grin2a
Grin2b
mRNA
increased.
intake
enhanced
ratio
phospho-GluA1/total-GluA1
proteins
subcellular
fraction
enriched
cytosolic
proteins,
while
decreased
content
GluA2
but
elevated
level
GluA3
observed
membrane
proteins.
Such
changes
accompanied
increased
phosphorylation
GluN2A
GluN2B
subunits,
higher
ratios
GluN2A/GluN1
GluN2B/GluN1
cytosol,
GluN2A/GluN2B
membranes.
These
indicate
predominance
Ca2+-permeable
AMPARs
membranes
shift
between
NMDARs
rats,
which
is
typical
neurodegeneration
can
least
partially
underly
disturbances
animals.
Studies in health technology and informatics,
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 23, 2023
To
study
the
effect
and
mechanism
of
electroacupuncture
at
"Baihui,
Yintang
Shuigou"
acupoints
on
learning
memory
in
Post-Stroke
Cognitive
Impairment
(PSCI)
mice.52
male
C57BL/6
mice
were
used
to
establish
a
MACO
model
by
using
middle
cerebral
artery
occlusion
(n=38),
while
Sham
only
ligated
distal
end
external
carotid
(n=14).
After
28
days,
MCAO
was
divided
into
three
groups
based
escape
latency
Morris
water
maze:
non
cognitive
impairment
(MNP),
post-stroke
(MP),
intervention
group
(MPEA).
In
MPEA,
"Baihui
Yintang"
performed
for
20
minutes
(density
wave,
2/15HZ
1mA)
supplemented
acupuncture
"Shuigou"
once
day
with
6-day
course
treatment.
The
last
2
courses
1-day
interval.
maze
detect
function
each
group;
Nissl
staining
observe
hippocampal
neurons;
Western
blot
expression
GluA1,
Syp,
Syt-1
affected
hippocampus;
IHC
Syp
CA1
region
contralateral
hippocampus.Acupuncture
points
Yintang,
can
improve
abilities
PSCI
mice,
its
is
related
synaptic
plasticity
hippocampus.
Digital Chinese Medicine,
Год журнала:
2023,
Номер
6(3), С. 295 - 306
Опубликована: Сен. 1, 2023
The
present
study
was
aimed
to
investigate
the
neuroprotective
effect
of
Croton
hirtus
(CH)
extract
against
streptozotocin
(STZ)
in
rats.
(i)
sub-chronic
toxicity
consisted
24
adult
rats
either
sex
weighing
from
160
200
g
were
divided
into
four
groups
with
six
each
group.
Rats
group
1
received
Dimethyl
sulfoxide
(DMSO)
mixed
saline;
2,
3,
and
4
100,
200,
400
mg/kg
methanolic
CH
(MECH)
orally
by
gavage
administration
for
28
d,
respectively.
functional
observation
battery
locomotor
activity
graded
as
part
their
neurobehavioral
brain
regions,
including
cortex
hippocampus,
analyzed
neuropathological
abnormalities.
(ii)
main
research
30
male
Wistar
g,
which
five
group,
control
(I),
STZ
(II),
Donepezil
(III),
MECH
(100
mg/kg,
IV),
(200
V)
groups.
I
citrate
buffer
DMSO
saline
14
d.
II
via
intracerebroventricular
injection
(3
bilateral
ICV-STZ)
on
days
3
followed
III,
IV,
V
[3
mg/(kg·d),
p.o.]
[100
tested
learning
memory
parameters
such
Morris
water
maze
(MWM)
passive
avoidance
test
(PAT).
They
sacrificed
after
completing
behavioural
experiments;
brains
harvested
estimate
acetylcholinesterase
(AChE),
lipid
peroxidation
(LPO),
glutathione
(GSH),
superoxide
dismutase
(SOD)
using
UV-Visible
Spectrophotometer;
caspase-3
evaluated
total
fluorescence
emission
spectra;
amyloid
β
(Aβ)
levels
detected
enzyme-linked
immuosorbent
assay
(ELISA);
histopathological
examination
conducted
CA1
region
hippocampus.
results
revealed
that
open
field
line
crossing,
rearing,
entering
middle
square,
defecating,
or
urinating
did
not
differ
significantly
(P
>
0.05).
show
any
lesions
neuronal
cells
inflammation
both
regions
compared
findings
demonstrated
STZ-treated
showed
a
significant
increase
impairment
<
0.001),
AChE,
caspase-3,
Aβ
(1-40
1-42),
LPO
increased
decrease
found
SOD
0.001)
GSH
0.01).
Moreover,
damage
In
contrast,
STZ-induced
biochemical
impairments
considerably
decreased
treatment
dose-dependently.
prevented
deficit
induced
due
antioxidant
action.
Restoration
cholinergic
functioning
may
be
cause
improvement.
Therefore,
able
treat
cognitive
disorders
like
Alzheimer's
disease
(AD).
