Clinical Chemistry and Laboratory Medicine (CCLM), Год журнала: 2023, Номер 0(0)
Опубликована: Ноя. 4, 2023
This study aimed to characterize serum lipid metabolism and identify potential biomarkers for compensated cirrhosis (CC) predicting decompensated (DC) discrimination using targeted quantitative lipidomics machine learning approaches.
Язык: Английский
Nature Reviews Genetics, Год журнала: 2023, Номер 25(1), С. 46 - 60
Опубликована: Июль 25, 2023
Язык: Английский
Процитировано
42
Journal of Lipid Research, Год журнала: 2024, Номер 65(10), С. 100631 - 100631
Опубликована: Авг. 24, 2024
Sphingosine kinases (SphKs), enzymes that produce the bioactive lipids dihydrosphingosine 1-phosphate (dhS1P) and sphingosine (S1P), are associated with various diseases, including cancer infections. For this reason, a number of SphK inhibitors have been developed. Although off-target effects described for selected agents, mostly used in research without monitoring on sphingolipidome. We now investigated seven commonly (5c, ABC294640 (opaganib), N,N-dimethylsphingosine, K145, PF-543, SLM6031434, SKI-II) profiles sphingolipids Chang, HepG2, human umbilical vein endothelial cells. While we observed expected (dh)S1P reduction SKI-II, 5c showed hardly any effect. Remarkably, K145 ABC294640, both reported to be specific SphK2, dose-dependent strong increases dhS1P S1P across cell lines. Compensatory SphK1 could excluded, as observation was also made SphK1-deficient HK-2 Furthermore, dihydroceramide desaturase activity all tested, has previously noted SKI-II. In additional mechanistic studies, massive increase after short-term treatment more detail. found compounds affect sphingolipid de novo synthesis, 3-ketodihydrosphingosine reductase their targets. Our study indicates none tested free unexpected on-target and/or effects. Therefore, it is important monitor cellular when studies.
Язык: Английский
Процитировано
8
Biochemistry (Moscow), Год журнала: 2024, Номер 89(S1), С. S57 - S70
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
7
Journal of Neurochemistry, Год журнала: 2025, Номер 169(2)
Опубликована: Фев. 1, 2025
ABSTRACT The modulation of the brain's electrical activity for therapeutic purposes has recently gained attention, supported by promising results obtained through non‐invasive application transcranial direct current stimulation (tDCS) in treatment neurodegenerative and neurological diseases. To optimize efficacy, it is crucial to investigate cellular molecular effects tDCS. This will help identify important biomarkers, predict patient's response develop personalized treatments. In this study, we applied (DCS) a neural cell line, using mild currents over short periods time (0.5 mA, 20 min), with 24‐h intervals. We observed that DCS induced changes lipidome, transient after single (lasting 24 h) more significant, long‐lasting (up 72 repeated cycles. cells, multiple modulated structural membrane lipids (PE, PS, PI), downregulated glycerol ether‐linked fatty acids pro‐inflammatory (ceramides lyso‐glycerophospholipids) ( p ≤ 0.005). Multiple sessions altered transcriptional decreasing expression inflammatory cytokines (TNF‐α, 0.05; IL‐1β, 0.01), while increasing neuroprotective factors such as heme oxygenase‐1 0.0001) brain‐derived neurotrophic factor 0.05), well proteins involved vesicular transport (SNARE, sorting nexins seipin α‐synuclein; 0.05). addition, enhanced release extracellular vesicles, stimulations significantly exosomes threefold. conclusion, induces metabolic limited phenotypic effects, applications induce broader deeper lipid species. may lead neuroplasticity‐focussed profile, potentially supporting tDCS at level patients.. image
Язык: Английский
Процитировано
1
Nature Metabolism, Год журнала: 2023, Номер 5(9), С. 1595 - 1614
Опубликована: Авг. 31, 2023
Язык: Английский
Процитировано
11
Journal of Cell Science, Год журнала: 2024, Номер 137(6)
Опубликована: Март 15, 2024
ABSTRACT Sphingolipid dysregulation is involved in a range of rare and fatal diseases as well common pathologies including cancer, infectious or neurodegeneration. Gaining insights into how sphingolipids are these would contribute much to our understanding human physiology, the pathology mechanisms. However, scientific progress hampered by lack suitable tools that can be used intact systems. To overcome this, efforts have turned engineering modified lipids with small clickable tags harnessing power click chemistry localize follow minimally lipid probes cells. We hope inspire readers this Review consider applying existing for their own aspects sphingolipid research. end, we focus here on different biological applications lipids, mainly metabolic conversions, visualization confocal superresolution microscopy identification protein interaction partners. Finally, describe recent approaches employing organelle-targeted accurately intracellular transport organellar precision.
Язык: Английский
Процитировано
4
Frontiers in Nutrition, Год журнала: 2024, Номер 11
Опубликована: Сен. 13, 2024
Neurofilament-light chain (NfL) is associated with neurodegenerative diseases, which are increasingly prevalent aging. Vitamin K has been shown a neuroprotective effect. Therefore, we aimed to explore the potential relationship between dietary vitamin intake and serum NfL.
Язык: Английский
Процитировано
3
Toxicology in Vitro, Год журнала: 2025, Номер unknown, С. 106058 - 106058
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Trends in Molecular Medicine, Год журнала: 2024, Номер 30(10), С. 913 - 923
Опубликована: Май 15, 2024
Язык: Английский
Процитировано
2
Vision Research, Год журнала: 2024, Номер 221, С. 108434 - 108434
Опубликована: Май 27, 2024
Язык: Английский
Процитировано
2