Targeted quantitative lipidomic uncovers lipid biomarkers for predicting the presence of compensated cirrhosis and discriminating decompensated cirrhosis from compensated cirrhosis DOI
Yongbin Zeng, Li Zhang,

Zhiyi Zheng

et al.

Clinical Chemistry and Laboratory Medicine (CCLM), Journal Year: 2023, Volume and Issue: 0(0)

Published: Nov. 4, 2023

This study aimed to characterize serum lipid metabolism and identify potential biomarkers for compensated cirrhosis (CC) predicting decompensated (DC) discrimination using targeted quantitative lipidomics machine learning approaches.

Language: Английский

Integrating non-mammalian model organisms in the diagnosis of rare genetic diseases in humans DOI
Shinya Yamamoto, Oguz Kanca, Michael F. Wangler

et al.

Nature Reviews Genetics, Journal Year: 2023, Volume and Issue: 25(1), P. 46 - 60

Published: July 25, 2023

Language: Английский

Citations

42
Direct Current Stimulation (DCS) Modulates Lipid Metabolism and Intercellular Vesicular Trafficking in SHSY‐5Y Cell Line: Implications for Parkinson's Disease DOI Creative Commons
Marco Piccoli,

Libero M. Barbato,

Natale Maiorana

et al.

Journal of Neurochemistry, Journal Year: 2025, Volume and Issue: 169(2)

Published: Feb. 1, 2025

ABSTRACT The modulation of the brain's electrical activity for therapeutic purposes has recently gained attention, supported by promising results obtained through non‐invasive application transcranial direct current stimulation (tDCS) in treatment neurodegenerative and neurological diseases. To optimize efficacy, it is crucial to investigate cellular molecular effects tDCS. This will help identify important biomarkers, predict patient's response develop personalized treatments. In this study, we applied (DCS) a neural cell line, using mild currents over short periods time (0.5 mA, 20 min), with 24‐h intervals. We observed that DCS induced changes lipidome, transient after single (lasting 24 h) more significant, long‐lasting (up 72 repeated cycles. cells, multiple modulated structural membrane lipids (PE, PS, PI), downregulated glycerol ether‐linked fatty acids pro‐inflammatory (ceramides lyso‐glycerophospholipids) ( p ≤ 0.005). Multiple sessions altered transcriptional decreasing expression inflammatory cytokines (TNF‐α, 0.05; IL‐1β, 0.01), while increasing neuroprotective factors such as heme oxygenase‐1 0.0001) brain‐derived neurotrophic factor 0.05), well proteins involved vesicular transport (SNARE, sorting nexins seipin α‐synuclein; 0.05). addition, enhanced release extracellular vesicles, stimulations significantly exosomes threefold. conclusion, induces metabolic limited phenotypic effects, applications induce broader deeper lipid species. may lead neuroplasticity‐focussed profile, potentially supporting tDCS at level patients.. image

Language: Английский

Citations

1
The sphingosine kinase 2 inhibitors ABC294640 and K145 elevate (dihydro)sphingosine 1-phosphate levels in various cells DOI Creative Commons

Agata Prell,

Dominik Wigger,

Andrea Huwiler

et al.

Journal of Lipid Research, Journal Year: 2024, Volume and Issue: 65(10), P. 100631 - 100631

Published: Aug. 24, 2024

Sphingosine kinases (SphKs), enzymes that produce the bioactive lipids dihydrosphingosine 1-phosphate (dhS1P) and sphingosine (S1P), are associated with various diseases, including cancer infections. For this reason, a number of SphK inhibitors have been developed. Although off-target effects described for selected agents, mostly used in research without monitoring on sphingolipidome. We now investigated seven commonly (5c, ABC294640 (opaganib), N,N-dimethylsphingosine, K145, PF-543, SLM6031434, SKI-II) profiles sphingolipids Chang, HepG2, human umbilical vein endothelial cells. While we observed expected (dh)S1P reduction SKI-II, 5c showed hardly any effect. Remarkably, K145 ABC294640, both reported to be specific SphK2, dose-dependent strong increases dhS1P S1P across cell lines. Compensatory SphK1 could excluded, as observation was also made SphK1-deficient HK-2 Furthermore, dihydroceramide desaturase activity all tested, has previously noted SKI-II. In additional mechanistic studies, massive increase after short-term treatment more detail. found compounds affect sphingolipid de novo synthesis, 3-ketodihydrosphingosine reductase their targets. Our study indicates none tested free unexpected on-target and/or effects. Therefore, it is important monitor cellular when studies.

Language: Английский

Citations

7
The Role of Vitamin K in the Development of Neurodegenerative Diseases DOI

Anna I. Diachenko,

И. А. Родин, Tatiana N. Krasnova

et al.

Biochemistry (Moscow), Journal Year: 2024, Volume and Issue: 89(S1), P. S57 - S70

Published: Jan. 1, 2024

Language: Английский

Citations

6
The cyanobacterial toxins BMAA and 2,4-DAB perturb the l-serine biosynthesis pathway and induce systemic changes in energy metabolism in human neuroblastoma cells: A proteomic study DOI Creative Commons
Lisa Pu, Joel R. Steele, Connor R. Phillips

et al.

Toxicology in Vitro, Journal Year: 2025, Volume and Issue: unknown, P. 106058 - 106058

Published: March 1, 2025

Language: Английский

Citations

0
A defect in mitochondrial fatty acid synthesis impairs iron metabolism and causes elevated ceramide levels DOI
Debdeep Dutta, Oguz Kanca, Seul Kee Byeon

et al.

Nature Metabolism, Journal Year: 2023, Volume and Issue: 5(9), P. 1595 - 1614

Published: Aug. 31, 2023

Language: Английский

Citations

10
The use of click chemistry in sphingolipid research DOI
Denisa Jamecna,

Doris Höglinger

Journal of Cell Science, Journal Year: 2024, Volume and Issue: 137(6)

Published: March 15, 2024

ABSTRACT Sphingolipid dysregulation is involved in a range of rare and fatal diseases as well common pathologies including cancer, infectious or neurodegeneration. Gaining insights into how sphingolipids are these would contribute much to our understanding human physiology, the pathology mechanisms. However, scientific progress hampered by lack suitable tools that can be used intact systems. To overcome this, efforts have turned engineering modified lipids with small clickable tags harnessing power click chemistry localize follow minimally lipid probes cells. We hope inspire readers this Review consider applying existing for their own aspects sphingolipid research. end, we focus here on different biological applications lipids, mainly metabolic conversions, visualization confocal superresolution microscopy identification protein interaction partners. Finally, describe recent approaches employing organelle-targeted accurately intracellular transport organellar precision.

Language: Английский

Citations

3
Dietary vitamin K intake is associated with decreased neurofilament light chain among middle-aged and older adults from the NHANES DOI Creative Commons
Jing Luo, Lin Song

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Sept. 13, 2024

Neurofilament-light chain (NfL) is associated with neurodegenerative diseases, which are increasingly prevalent aging. Vitamin K has been shown a neuroprotective effect. Therefore, we aimed to explore the potential relationship between dietary vitamin intake and serum NfL.

Language: Английский

Citations

3
Unveiling enigmatic essence of Sphingolipids: A promising avenue for glaucoma treatment DOI
Igor Iezhitsa, Renu Agarwal, Puneet Agarwal

et al.

Vision Research, Journal Year: 2024, Volume and Issue: 221, P. 108434 - 108434

Published: May 27, 2024

Language: Английский

Citations

2
Glycosphingolipids in human parasites DOI Creative Commons
Richard D. Cummings

FEBS Open Bio, Journal Year: 2023, Volume and Issue: 13(9), P. 1625 - 1635

Published: June 19, 2023

Glycosphingolipids (GSLs) are comprised of glycans (oligosaccharides) linked to a lipid containing sphingosine moiety. They major membrane components in cells most animals, and importantly, they also occur parasitic protozoans worms that infect people. While the endogenous functions GSLs parasites elusive, many these recognized by antibodies infected human animal hosts, thus, their structures, biosynthesis, great interest. Such knowledge could lead new drugs diagnostics for treating infections, as well novel vaccine strategies. The diversity recently identified such infectious organisms aspects immune recognition topics this review. It is not intended be exhaustive but highlight GSL parasites.

Language: Английский

Citations

5