Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 7, 2025

Low-mass soluble β-amyloid oligomers (LSAβO) are critical Alzheimer's disease (AD) biomarkers with significant diagnostic and therapeutic potential. However, their application in early screening detection is limited by the reliance on complex analytical instruments procedures. To address this, we developed a visual sensing platform for LSAβO using functionalized pNIPAM-DNA hydrogel. Exploiting temperature-responsive nature of pNIPAM, hydrogel selectively incorporates enriches from solution via temperature-induced expansion contraction. binding to aptamers triggers formation G-quadruplex DNAzymes, which catalyze oxidation 3,3',5,5'-tetramethylbenzidine generate visible colorimetric signal. The hydrogel's small pore size further enhances selectivity excluding larger during real sample analysis. This sensor exhibits linear range 0.1-7.5 nM limit 50 pM. Combining enrichment exclusion, this provides cost-effective, highly sensitive, selective, high-throughput approach preliminary detection.

Язык: Английский