Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 7, 2025
Low-mass
soluble
β-amyloid
oligomers
(LSAβO)
are
critical
Alzheimer's
disease
(AD)
biomarkers
with
significant
diagnostic
and
therapeutic
potential.
However,
their
application
in
early
screening
detection
is
limited
by
the
reliance
on
complex
analytical
instruments
procedures.
To
address
this,
we
developed
a
visual
sensing
platform
for
LSAβO
using
functionalized
pNIPAM-DNA
hydrogel.
Exploiting
temperature-responsive
nature
of
pNIPAM,
hydrogel
selectively
incorporates
enriches
from
solution
via
temperature-induced
expansion
contraction.
binding
to
aptamers
triggers
formation
G-quadruplex
DNAzymes,
which
catalyze
oxidation
3,3',5,5'-tetramethylbenzidine
generate
visible
colorimetric
signal.
The
hydrogel's
small
pore
size
further
enhances
selectivity
excluding
larger
during
real
sample
analysis.
This
sensor
exhibits
linear
range
0.1-7.5
nM
limit
50
pM.
Combining
enrichment
exclusion,
this
provides
cost-effective,
highly
sensitive,
selective,
high-throughput
approach
preliminary
detection.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 7, 2025
Low-mass
soluble
β-amyloid
oligomers
(LSAβO)
are
critical
Alzheimer's
disease
(AD)
biomarkers
with
significant
diagnostic
and
therapeutic
potential.
However,
their
application
in
early
screening
detection
is
limited
by
the
reliance
on
complex
analytical
instruments
procedures.
To
address
this,
we
developed
a
visual
sensing
platform
for
LSAβO
using
functionalized
pNIPAM-DNA
hydrogel.
Exploiting
temperature-responsive
nature
of
pNIPAM,
hydrogel
selectively
incorporates
enriches
from
solution
via
temperature-induced
expansion
contraction.
binding
to
aptamers
triggers
formation
G-quadruplex
DNAzymes,
which
catalyze
oxidation
3,3',5,5'-tetramethylbenzidine
generate
visible
colorimetric
signal.
The
hydrogel's
small
pore
size
further
enhances
selectivity
excluding
larger
during
real
sample
analysis.
This
sensor
exhibits
linear
range
0.1-7.5
nM
limit
50
pM.
Combining
enrichment
exclusion,
this
provides
cost-effective,
highly
sensitive,
selective,
high-throughput
approach
preliminary
detection.
