Phytochemical constituent analysis, antioxidative activity, and cytotoxic effects of Caulerpa lentillifera extracts inducing cell apoptosis and sub-G/G0-G1 cell cycle arrest in KON oral cancer cells DOI Creative Commons

Suwisit Manmuan,

Sukannika Tubtimsri, Thanchanok Sirirak

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Дек. 9, 2024

Abstract Background The purpose of this study was to explore the anti-cancer properties Caulerpa lentillifera (C. lentillifera) in order gain insights into possible treatment approaches. Using maceration method, which included solubilizing chemicals such as methanol, ethanol, and acetone, C. extracted. chemical composition acetone extracts investigated their anti-oxidative activity evaluated. Methods phytoconstituents were screened using LC-QTOF MS/MS technique, cytotoxic effect assessed MTT test. We used Hoechst 33258 AO double staining investigate morphological changes apoptosis, DAPI FDA labeling look at nucleus cytoplasm. a flow cytometer, percentage cell cycle arrest calculated fraction death examined. Results analyses results revealed ten main phytochemicals extracts. KON cells exhibited sensitivity methanol that order. By causing ROS-mediated death, concentrations extract IC40, IC60, IC80 can cause apoptosis less than day. prevented from advancing past sub-G G0-G1 stages by all three Furthermore, significantly impeded colony growth, migration, invasion. increase cellular uptake measured TEER Conclusion This work advances our understanding anti-tumor characteristics extract, will benefit patients suffering oral cancer.

Язык: Английский

Culturing delicacies: Potential to integrate the gastropod Babylonia areolata into pond cultures of Caulerpa lentillifera DOI Creative Commons
Beatrice Brix da Costa, Lara Elisabeth Stuthmann,

Aaron Johannes Cordes

и другие.

Aquaculture Reports, Год журнала: 2023, Номер 33, С. 101793 - 101793

Опубликована: Окт. 25, 2023

In Van Phong Bay, Viet Nam, the economically important macroalgae sea grapes (Caulerpa lentillifera; Caulerpaceae, Bryopsidales) and spotted babylon snail (Babylonia areolata) are cultivated in tidal ponds close proximity. A co-culture same pond could benefit farmers by saving space mitigating potential eutrophication. The study assessed of snails held within system. an outdoor experiment, three different treatments (algae together, algae spatially separated, only algae) were for their effect on water quality, biomass properties size increase both organisms, photosynthetic efficiency, antioxidant activity, total phenolic content survival snails. a second (indoor) influence densities physiological state was investigated. experiments, presence raised concentrations nitrogen oxides (NOx) phosphate (PO4). had positive growth physiology grapes. Sea trays co-cultured with Babylonia showed highest biomass. not affected spatial separation alga high stocking be implemented without negatively affecting seaweed. results promising regarding establishment co-cultures indicate economic feasibility integrating B. areolata into C. lentillifera cultures.

Язык: Английский

Процитировано

2
Gracilaria chorda attenuates obesity‐related muscle wasting through activation of SIRT1/PGC1α in skeletal muscle of mice DOI Creative Commons
Ahyoung Yoo, Jiyun Ahn,

Hyo Deok Seo

и другие.

Food Science & Nutrition, Год журнала: 2024, Номер 12(7), С. 5077 - 5086

Опубликована: Апрель 4, 2024

(GC) is a red algal species that primarily consumed in Asia. Here, we investigated the effect of GC on obesity-related skeletal muscle wasting. Furthermore, elucidating its impact activation sirtuin 1 (SIRT1)/peroxisome proliferator-activated receptor gamma coactivator 1α (PGC1α) constituted critical aspect understanding underlying mechanism action. In this study, 6-week-old male C57BL/6 mice were fed high-fat diet (HFD) for 8 weeks to induce obesity, then continued HFD another while orally administered GC. decreased ectopic fat accumulation and increased weight, size, function obese mice. reduced atrophy hypertrophy We hypothesized SIRT1/PGC1α by regulates hypertrophy. observed expression SIRT1 PGC1α C2C12 cells, which mitochondrial biogenesis. addition, when cells treated with SIRT1-specific inhibitor EX-527, no changes protein levels GC-treated cells. Therefore, attenuated wasting improving biogenesis through

Язык: Английский

Процитировано

0
A comparative analysis on impact of drying methods on antioxidants, antidiabetes and antiobesity activities in green algae Caulerpa lentillifera: In vitro study DOI
Roike Iwan Montolalu, Verly Dotulong, Feny Mentang

и другие.

Algal Research, Год журнала: 2024, Номер 84, С. 103768 - 103768

Опубликована: Окт. 22, 2024

Язык: Английский

Процитировано

0
Caulerpa lentillifera Sea Grape: A Promising Source of Bioactive Metabolites for Aquaculture and Health DOI
Muhammad Dawood Shah, Fui Fui Ching, Wahidatul Husna Zuldin

и другие.

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

0
Phytochemical constituent analysis, antioxidative activity, and cytotoxic effects of Caulerpa lentillifera extracts inducing cell apoptosis and sub-G/G0-G1 cell cycle arrest in KON oral cancer cells DOI Creative Commons

Suwisit Manmuan,

Sukannika Tubtimsri, Thanchanok Sirirak

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Дек. 9, 2024

Abstract Background The purpose of this study was to explore the anti-cancer properties Caulerpa lentillifera (C. lentillifera) in order gain insights into possible treatment approaches. Using maceration method, which included solubilizing chemicals such as methanol, ethanol, and acetone, C. extracted. chemical composition acetone extracts investigated their anti-oxidative activity evaluated. Methods phytoconstituents were screened using LC-QTOF MS/MS technique, cytotoxic effect assessed MTT test. We used Hoechst 33258 AO double staining investigate morphological changes apoptosis, DAPI FDA labeling look at nucleus cytoplasm. a flow cytometer, percentage cell cycle arrest calculated fraction death examined. Results analyses results revealed ten main phytochemicals extracts. KON cells exhibited sensitivity methanol that order. By causing ROS-mediated death, concentrations extract IC40, IC60, IC80 can cause apoptosis less than day. prevented from advancing past sub-G G0-G1 stages by all three Furthermore, significantly impeded colony growth, migration, invasion. increase cellular uptake measured TEER Conclusion This work advances our understanding anti-tumor characteristics extract, will benefit patients suffering oral cancer.

Язык: Английский

Процитировано

0