Cancers,
Год журнала:
2022,
Номер
14(19), С. 4801 - 4801
Опубликована: Сен. 30, 2022
At
present,
most
patients
with
oral
squamous
cell
carcinoma
(OSCC)
are
in
the
middle
or
advanced
stages
at
time
of
diagnosis.
Advanced
OSCC
have
a
poor
prognosis
after
traditional
therapy,
and
complex
heterogeneity
has
been
proven
to
be
one
main
reasons.
Single-cell
sequencing
technology
provides
powerful
tool
for
dissecting
cancer.
However,
current
studies
single-cell
level
static,
while
development
cancer
is
dynamic
process.
Thus,
understanding
from
perspective
formulating
corresponding
therapeutic
measures
achieving
precise
treatment
highly
necessary,
this
also
study
directions
field
oncology.
In
study,
we
combined
static
analysis
methods
based
on
RNA-Seq
data
comprehensively
dissect
evolutionary
process
OSCC.
Subsequently,
clinical
practice,
revealed
association
between
patients.
More
importantly,
pioneered
concept
pseudo-time
score
patients,
quantified
levels
evaluate
relationship
survival
status
same
stage,
finding
that
it
closely
related
prognostic
status.
The
could
not
only
reflect
tumor
but
used
as
an
indicator
effects
drugs
so
medication
strategy
can
adjusted
time.
Finally,
identified
candidate
proposed
precision
strategies
control
condition
two
respects:
blocking.
Biomedical and Biotechnology Research Journal (BBRJ),
Год журнала:
2024,
Номер
8(3), С. 328 - 339
Опубликована: Июль 1, 2024
Abstract
Background:
Histone
deacetylase
(HDAC)
enzymes
play
a
crucial
role
in
regulating
gene
expression
and
epigenetic
alterations
cancer
cells.
Mocetinostat
(MGCD0103)
is
novel,
isotype-selective
HDAC
inhibitor
that
targets
Class
I
(HDAC1,
2,
3,
8)
IV
(HDAC11)
enzymes.
It
has
been
approved
for
the
use
phase
II
trials
Hodgkin’s
lymphoma.
Methods:
In
this
study,
glioblastoma
cell
(GBM)
lines
T98G
C6
were
treated
with
different
concentrations
of
MGCD0103
(0.0,
0.5,
1.0,
1.5,
2.0,
2.5
μM).
Western
blot
analysis
was
used
to
evaluate
protein
flow
cytometry
employed
assess
apoptosis.
Results:
The
results
demonstrated
exerts
multiple
anti-cancer
activities
GBMs.
modulated
key
signaling
pathways,
including
inhibition
Phosphatidylinositol
3-
kinase
(PI3K)/protein
B
mechanism
pathway
suppression
HDAC1
enzyme
activity.
High
doses
significantly
induced
apoptosis
suppressed
proliferation
by
upregulating
pro-apoptotic
Bcl-2-associated
x
downregulating
anti-apoptotic
proteins
BH3
Interacting
Domain
Death
Agonist
B-cell
leukemia/lymphoma
2
protein.
addition,
treatment
upregulated
tumor-suppressor
downregulated
E2F1
transcription
factor.
Furthermore,
facilitated
differentiation
activating
glial
fibrillary
acidic
Glial
Fibrillary
(GFAP)
as
distinguish
marker
astrocytes,
suppressing
undifferentiation
markers
Inhibitor
Deoxyribonucleic
acid
binding
N-Myc
proto-oncogene
Conclusion:
This
research
suggests
promising
drug
inhibiting
proliferation,
invasion,
migration
findings
also
provide
new
insights
into
ability
induce
Overall,
these
indicate
could
be
potent
therapeutic
agent
target
glioblastoma.
Cancers,
Год журнала:
2022,
Номер
14(19), С. 4801 - 4801
Опубликована: Сен. 30, 2022
At
present,
most
patients
with
oral
squamous
cell
carcinoma
(OSCC)
are
in
the
middle
or
advanced
stages
at
time
of
diagnosis.
Advanced
OSCC
have
a
poor
prognosis
after
traditional
therapy,
and
complex
heterogeneity
has
been
proven
to
be
one
main
reasons.
Single-cell
sequencing
technology
provides
powerful
tool
for
dissecting
cancer.
However,
current
studies
single-cell
level
static,
while
development
cancer
is
dynamic
process.
Thus,
understanding
from
perspective
formulating
corresponding
therapeutic
measures
achieving
precise
treatment
highly
necessary,
this
also
study
directions
field
oncology.
In
study,
we
combined
static
analysis
methods
based
on
RNA-Seq
data
comprehensively
dissect
evolutionary
process
OSCC.
Subsequently,
clinical
practice,
revealed
association
between
patients.
More
importantly,
pioneered
concept
pseudo-time
score
patients,
quantified
levels
evaluate
relationship
survival
status
same
stage,
finding
that
it
closely
related
prognostic
status.
The
could
not
only
reflect
tumor
but
used
as
an
indicator
effects
drugs
so
medication
strategy
can
adjusted
time.
Finally,
identified
candidate
proposed
precision
strategies
control
condition
two
respects:
blocking.
