Materials Today Bio, Год журнала: 2025, Номер 32, С. 101766 - 101766

Опубликована: Апрель 12, 2025

In the face of advancements in osteosarcoma research, existing preclinical models - including vitro (i.e., two- and three-dimensional cell cultures, organoids) vivo approaches xenografts, animal models) are often characterised by low translatability, limiting their predictive power for clinical outcomes. This study investigated potential use a 3D-printed β-tricalcium phosphate (β-TCP) scaffold as bone-mimicking environment an advanced model. The compatibility with spheroids, endothelial cells, primary bone marrow-derived mesenchymal stem cells (pBMSCs) was evaluated along its physicochemical characteristics. Transcriptomic analysis pBMSCs on scaffolds revealed gene expression profiles indicating pronounced extracellular matrix organisation minor osteogenic activity. model effectively replicated significant aspects tumour microenvironment tri-culture system, dynamic perfusion enhancing metabolic developed scaffold-based employed doxorubicin cytotoxicity test. physiological significance demonstrated distinct accumulation, contrast to spheroid monocultures. Despite limitations proposed approach regarding efficient vascularisation model, this highlights β-TCP modelling support physiologically relevant models.

Язык: Английский