3D-Printed β-TCP Scaffold as a Bone-Mimicking Environment for an Engineered Model of Osteosarcoma: In Vitro Properties and Transcriptomic Insights DOI Creative Commons
Ksenia Menshikh, Virginia Alessandra Gobbo, Mauro Nascimben

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 32, P. 101766 - 101766

Published: April 12, 2025

In the face of advancements in osteosarcoma research, existing preclinical models - including vitro (i.e., two- and three-dimensional cell cultures, organoids) vivo approaches xenografts, animal models) are often characterised by low translatability, limiting their predictive power for clinical outcomes. This study investigated potential use a 3D-printed β-tricalcium phosphate (β-TCP) scaffold as bone-mimicking environment an advanced model. The compatibility with spheroids, endothelial cells, primary bone marrow-derived mesenchymal stem cells (pBMSCs) was evaluated along its physicochemical characteristics. Transcriptomic analysis pBMSCs on scaffolds revealed gene expression profiles indicating pronounced extracellular matrix organisation minor osteogenic activity. model effectively replicated significant aspects tumour microenvironment tri-culture system, dynamic perfusion enhancing metabolic developed scaffold-based employed doxorubicin cytotoxicity test. physiological significance demonstrated distinct accumulation, contrast to spheroid monocultures. Despite limitations proposed approach regarding efficient vascularisation model, this highlights β-TCP modelling support physiologically relevant models.

Language: Английский

Gastrointestinal cancer: Current status of preclinical research DOI
Nariman Shahid, Sana Hanif,

Muhammad Ali Syed

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 317 - 348

Published: Jan. 1, 2025

Language: Английский

Citations

0
3D-Printed β-TCP Scaffold as a Bone-Mimicking Environment for an Engineered Model of Osteosarcoma: In Vitro Properties and Transcriptomic Insights DOI Creative Commons
Ksenia Menshikh, Virginia Alessandra Gobbo, Mauro Nascimben

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: 32, P. 101766 - 101766

Published: April 12, 2025

In the face of advancements in osteosarcoma research, existing preclinical models - including vitro (i.e., two- and three-dimensional cell cultures, organoids) vivo approaches xenografts, animal models) are often characterised by low translatability, limiting their predictive power for clinical outcomes. This study investigated potential use a 3D-printed β-tricalcium phosphate (β-TCP) scaffold as bone-mimicking environment an advanced model. The compatibility with spheroids, endothelial cells, primary bone marrow-derived mesenchymal stem cells (pBMSCs) was evaluated along its physicochemical characteristics. Transcriptomic analysis pBMSCs on scaffolds revealed gene expression profiles indicating pronounced extracellular matrix organisation minor osteogenic activity. model effectively replicated significant aspects tumour microenvironment tri-culture system, dynamic perfusion enhancing metabolic developed scaffold-based employed doxorubicin cytotoxicity test. physiological significance demonstrated distinct accumulation, contrast to spheroid monocultures. Despite limitations proposed approach regarding efficient vascularisation model, this highlights β-TCP modelling support physiologically relevant models.

Language: Английский

Citations

0