Mir22hg facilitates ferritinophagy-mediated ferroptosis in sepsis by recruiting the m6A reader YTHDC1 and enhancing Angptl4 mRNA stability DOI Creative Commons

Wenlong Deng,

Liang Zhong,

Shupei Ye

и другие.

Journal of Bioenergetics and Biomembranes, Год журнала: 2024, Номер 56(4), С. 405 - 418

Опубликована: Июнь 6, 2024

Abstract Background Ferritinophagy-mediated ferroptosis plays a crucial role in fighting pathogen aggression. The long non-coding RNA Mir22hg is involved the regulation of and aberrantly overexpression lipopolysaccharide (LPS)-induced sepsis mice, but whether it regulates through ferritinophagy-mediated unclear. Methods was screened by bioinformatics analysis. Ferroptosis assessed assaying malondialdehyde (MDA), reactive oxygen species (ROS), Fe 2+ levels, glutathione (GSH) activity, as well ferroptosis-related proteins GPX4 SLC3A2 using matched kits performing western blot. Ferritinophagy Lyso tracker staining FerroOrange staining, immunofluorescence analysis Ferritin LC-3, blot LC-3II/I, p62, FTH1, NCOA4. bind YTH domain containing 1 (YTHDC1) to or angiopoietin-like-4 (Angptl4) verified pull-down and/or immunoprecipitation (RIP) assays. Results silencing lightened ferritinophagy LPS-induced MLE-12 cells mouse models, presented downregulated MDA, ROS, , NCOA4, upregulated GPX4, GSH, FTH1 along with decrease autophagy. could m6A reader YTHDC1 without affecting its expression. Mechanistically, enhanced Angptl4 mRNA stability recruiting YTHDC1. Furthermore, partly overturned inhibition-mediated effects on cells. Conclusion contributed via strengthening stability, highlighting that may be potential target for treatment based ferroptosis.

Язык: Английский

Autophagy: Regulator of cell death DOI Creative Commons
ShiZuo Liu,

ShuaiJie Yao,

Huan Yang

и другие.

Cell Death and Disease, Год журнала: 2023, Номер 14(10)

Опубликована: Окт. 4, 2023

Abstract Autophagy is the process by which cells degrade and recycle proteins organelles to maintain intracellular homeostasis. Generally, autophagy plays a protective role in cells, but disruption of mechanisms or excessive autophagic flux usually leads cell death. Despite recent progress study regulation underlying molecular autophagy, numerous questions remain be answered. How does regulate death? What are fine-tuned regulatory autophagy-dependent death (ADCD) autophagy-mediated (AMCD)? In this article, we highlight different roles discuss six main autophagy-related modalities, with focus on metabolic changes caused endoplasmic reticulum-phagy (ER-phagy)-induced mitophagy ferroptosis. Finally, enhancement treatment diseases offer new perspective based use for functional conversions (including conversion that modalities) clinical tumors.

Язык: Английский

Процитировано

347
Targeting Nrf2 for ferroptosis-based therapy: Implications for overcoming ferroptosis evasion and therapy resistance in cancer DOI Creative Commons
Jaewang Lee, Jong‐Lyel Roh

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Год журнала: 2023, Номер 1869(7), С. 166788 - 166788

Опубликована: Июнь 10, 2023

Язык: Английский

Процитировано

31
Acmella oleracea (L.) R.K. Jansen essential oils: Chemical composition, antioxidant, and cytotoxic activities DOI
Lucas Botelho Jerônimo, Paulo Vinicius Lima Santos, Laine Celestino Pinto

и другие.

Biochemical Systematics and Ecology, Год журнала: 2024, Номер 112, С. 104775 - 104775

Опубликована: Янв. 5, 2024

Язык: Английский

Процитировано

13
Current Progress of Ferroptosis Study in Hepatocellular Carcinoma DOI Creative Commons
Xinyue Zhu,

Xudong Sha,

Yan Zang

и другие.

International Journal of Biological Sciences, Год журнала: 2024, Номер 20(9), С. 3621 - 3637

Опубликована: Янв. 1, 2024

Ferroptosis, an emerging type of programmed cell death, is initiated by iron-dependent and excessive ROS-mediated lipid peroxidation, which eventually leads to plasma membrane rupture death. Many canonical signalling pathways biological processes are involved in ferroptosis. Furthermore, cancer cells more susceptible ferroptosis due the high load ROS unique metabolic characteristics, including iron requirements. Recent investigations have revealed that plays a crucial role progression tumours, especially HCC. Specifically, induction can not only inhibit growth hepatoma cells, thereby reversing tumorigenesis, but also improves efficacy immunotherapy enhances antitumour immune response. Therefore, triggering has become new therapeutic strategy for therapy. In this review, we summarize characteristics based on its underlying mechanism HCC provide possible applications.

Язык: Английский

Процитировано

12
Ferroptosis targeting natural compounds as a promising approach for developing potent liver cancer agents DOI Creative Commons
Pratibha Pandey,

Deena Elsori,

Rahul Kumar

и другие.

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Апрель 26, 2024

Liver cancer is the second leading cause of cancer-related death worldwide. However, treatment options, including surgical resection, transplantation, and molecular drug therapies, are limited effectiveness. Recent studies have demonstrated that suppressing ferroptosis might be a pivotal signal for liver initiation, thus providing new way to combat cancer. Ferroptosis distinct form controlled cell differs from conventional routes like apoptosis, necrosis, pyroptosis. It results intracellular iron overload, which raises iron-dependent reactive oxygen species. This, in turn, leads accumulation lipid peroxides further result oxidative damage membranes, disrupt normal functioning, ultimately speed up phenomenon. regulation intricately linked cellular physiological processes, encompassing metabolism, equilibrium between oxygen-free radical reactions peroxidation. This review intends summarize natural compounds targeting offer therapeutic ideas Furthermore, it serves as foundation identifying applying chemical medicines chemicals target treat efficiently.

Язык: Английский

Процитировано

10
METTL3-mediated m6A modification of SLC7A11 enhances nasopharyngeal carcinoma radioresistance by inhibiting ferroptosis DOI Creative Commons

Zili Dai,

Baisheng Lin,

Minghui Qin

и другие.

International Journal of Biological Sciences, Год журнала: 2025, Номер 21(4), С. 1837 - 1851

Опубликована: Фев. 10, 2025

Radiotherapy is the primary treatment for nasopharyngeal carcinoma (NPC); nonetheless, radioresistance remains leading cause of localized recurrence. Our study demonstrates a significant increase in N6-methyladenosine (m6A) methylase METTL3 NPC and other tumors. Mechanistically, acts as an m6A methylase, enhancing modification solute carrier family 7 member 11 (SLC7A11) transcript, which increases its stability expression, thereby inhibiting radiation-induced ferroptosis ultimately inducing NPC. Furthermore, silencing SLC7A11 or employing inducer Erastin negated promoting effect on cell radioresistance. These findings suggest that could be novel therapeutic target overcoming radiotherapy resistance

Язык: Английский

Процитировано

1
Curcumol alleviates liver fibrosis through inducing autophagy and ferroptosis in hepatic stellate cells DOI
Yang Zheng, Tiejian Zhao,

Jiaru Wang

и другие.

The FASEB Journal, Год журнала: 2022, Номер 36(12)

Опубликована: Ноя. 18, 2022

To explore the effect of curcumol on autophagy and ferroptosis hepatic stellate cells, to clarify molecular mechanism its anti-hepatic fibrosis. In present study, we report that promotes death activated HSCs reduces deposition extracellular matrix. Interestingly, treatment can trigger eliminate characterized by iron overload, lipid ROS accumulation, glutathione depletion, peroxidation. Curcumol HSC autophagy, which may be key for induction ferroptosis. It is worth noting upregulation nuclear receptor coactivator 4 (NCOA4) play a mechanism. NCOA4 mediates release ions induces occurrence Overall, in degradation FTH1 complexes, releases ions, leads ferroptosis, an important fibrosis effect.

Язык: Английский

Процитировано

35
Schisandrin B promotes senescence of activated hepatic stellate cell via NCOA4-mediated ferritinophagy DOI Creative Commons
Mingyue Ma, Na Wei,

Yang Jie-ren

и другие.

Pharmaceutical Biology, Год журнала: 2023, Номер 61(1), С. 621 - 629

Опубликована: Апрель 3, 2023

Schisandrin B (Sch B), an active ingredient from Schisandrae chinensis (Turcz.) Baill. (Schisandraceae) Fructus, possesses diverse pharmacological activities including antitumor, anti-inflammation, and hepatoprotection.To explore the effect of Sch on activated HSCs senescence in hepatic fibrosis mechanisms implicated.ICR mice with CCl4-induced were supplemented (40 mg/kg) for 30 d LX2 cells treated (5, 10 20 μM) 24 h. Cellular was assessed by senescence-related indicators senescence-associated β-galactosidase (SA-β-gal) activity expression p16, p21, p53, γ-H2AX, H3K9me3, TERT, TRF1, TRF2. Ferric ammonium citrate (FAC) NCOA4 siRNA used to evaluate underlying B's regulation cellular senescence.Sch reduced serum levels AST ALT (53.2% 63.6%), alleviated collagen deposition, promoted mice. Treatment (20 decreased cell viability 80.38 ± 4.87% elevated SA-β-gal activity, p21 p53 increased 4.5-, 2.9-, 3.5-fold TRF1 TRF2 2.4-, 2.7-, 2.6-fold cells. FAC (400 enhanced mentioned above. weakened effects iron deposition could ameliorate through promotion senescence, which might be attributed its induction NCOA4-mediated ferritinophagy subsequent overload.

Язык: Английский

Процитировано

21
Ferritinophagy in the etiopathogenic mechanism of related diseases DOI Open Access
Xuemei Jin, Chunjie Jiang,

Zhizhou Zou

и другие.

The Journal of Nutritional Biochemistry, Год журнала: 2023, Номер 117, С. 109339 - 109339

Опубликована: Апрель 13, 2023

Язык: Английский

Процитировано

21
Targeting autophagy drug discovery: Targets, indications and development trends DOI

Mengjia Jiang,

Wayne Wu,

Zijie Xiong

и другие.

European Journal of Medicinal Chemistry, Год журнала: 2024, Номер 267, С. 116117 - 116117

Опубликована: Янв. 23, 2024

Язык: Английский

Процитировано

8