International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
24(1), С. 241 - 241
Опубликована: Дек. 23, 2022
The
study
of
diseases
the
central
nervous
system
(CNS)
at
molecular
level
is
challenging
because
complexity
neural
circuits
and
huge
number
specialized
cell
types.
Moreover,
genomic
association
studies
have
revealed
complex
genetic
architecture
schizophrenia
other
genetically
determined
mental
disorders.
Investigating
such
to
decipher
basis
CNS
pathologies
requires
use
high-throughput
models
as
cells
their
derivatives.
time
coming
for
technologies
based
on
CRISPR
(Clustered
Regularly
Interspaced
Short
Palindromic
Repeat)/Cas
systems
manipulate
multiple
targets.
CRISPR/Cas
provide
desired
complexity,
versatility,
flexibility
create
novel
tools
capable
both
altering
DNA
sequence
affecting
its
function
higher
levels
information
flow.
make
it
possible
find
investigate
intricate
relationship
between
genotype
phenotype
neuronal
cells.
purpose
this
review
discuss
innovative
CRISPR-based
approaches
studying
mechanisms
using
cellular
models.
Cells,
Год журнала:
2023,
Номер
12(8), С. 1181 - 1181
Опубликована: Апрель 18, 2023
Human-relevant
three-dimensional
(3D)
models
of
cerebral
tissue
can
be
invaluable
tools
to
boost
our
understanding
the
cellular
mechanisms
underlying
brain
pathophysiology.
Nowadays,
accessibility,
isolation
and
harvesting
human
neural
cells
represents
a
bottleneck
for
obtaining
reproducible
accurate
gaining
insights
in
fields
oncology,
neurodegenerative
diseases
toxicology.
In
this
scenario,
given
their
low
cost,
ease
culture
reproducibility,
cell
lines
constitute
key
tool
developing
usable
reliable
brain.
Here,
we
review
most
recent
advances
3D
constructs
laden
with
lines,
highlighting
advantages
limitations
possible
future
applications.
ACS Pharmacology & Translational Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 30, 2025
Glioblastoma
multiforme
(GBM)
is
highly
aggressive,
necessitating
new
therapies.
Photoactivated
chemotherapy
(PACT)
offers
a
promising
approach
by
activating
prodrugs
with
visible
light
at
the
tumor
site.
This
study
evaluated
anticancer
activity
of
ruthenium-based
PACT
compounds
in
U-87MG
glioblastoma
cells
and
their
safety
SH-SY5Y
neuron-like
cells.
The
compound
[3](PF6)2
showed
light-activated
effects
cells,
while
[1](PF6)2
was
inactive,
[2](PF6)2
nonactivated.
Interestingly,
increased
cell
proliferation,
similar
to
donepezil,
without
causing
death.
Increased
Ca2+
uptake
observed,
possibly
via
interaction
AMPA
receptor,
as
suggested
docking
studies.
These
findings
suggest
may
serve
potential
treatments
for
GBM,
effectively
attacking
cancer
preserving
healthy
neuronal
Journal of Neuroendocrinology,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 19, 2025
Abstract
Post‐traumatic
stress
disorder
(PTSD)
and
major
depressive
(MDD)
are
debilitating
stress‐related
psychiatric
disorders
that
can
develop
following
exposure
to
traumatic
events
or
chronic
in
some
individuals.
The
neurobiological
processes
leading
disease
remain
largely
unknown.
Among
others,
these
characterized
by
a
dysregulated
hypothalamic–pituitary–adrenal
axis,
which
is
regulated
the
glucocorticoid
receptor
(GR)
mineralocorticoid
(MR).
This
leads
altered
downstream
corticosteroid‐induced
gene
expression.
In
vitro
models
promising
tools
investigate
specific
underpinnings
of
response
brain.
Here,
we
investigated
suitability
SH‐SY5Y‐derived
neurons
as
cost‐efficient
system
study
role
GR
MR
neuronal
response.
were
characterized,
exposed
corticosteroids,
analyzed
on
transcriptomic
proteomic
levels.
We
show
(i)
express
sufficient
seemingly
functional
allow
transcription,
(ii)
three
corticosteroids
cortisol,
dexamethasone,
aldosterone,
induced
similar
effects,
(iii)
antagonist
spironolactone
mildly
attenuated
effects
dexamethasone
FKBP5
,
DUSP1
SUPV3L1
.
Mifepristone
did
not
significantly
alter
effect
aldosterone.
(iv)
Integrating
alterations
corticosteroid‐exposed
with
those
iPSC‐derived
showed
concordant
two
systems.
To
determine
translational
validity,
compared
expression
transcriptome
postmortem
brain
samples
from
individuals
PTSD
MDD,
yielding
stronger
negative
correlations
corticosteroid
signatures
than
MDD
signatures.
Upon
further
refinement
validation,
may
serve
simplistic
tool
implicated
molecular
networks
around
MR.
Strengthening
our
insight
into
receptors'
functions
improves
understanding
commonly
such
MDD.
IntechOpen eBooks,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 20, 2025
Neuroinflammation
is
an
inflammatory
response
that
affects
the
central
nervous
system.
This
process
involves
activation
of
immune
cells
like
microglia
and
astrocytes,
as
well
production
chemicals
cytokines
chemokines.
can
be
caused
by
a
variety
circumstances,
including
trauma,
infection,
autoimmune
illnesses,
environmental
factors,
any
stress
scenario,
neurodegenerative
diseases.
thought
to
connected
with
psychiatric
disorders.
These
illnesses
include
depression,
anxiety
disorders,
schizophrenia,
bipolar
disorder.
Research
in
biological
neuropsychiatry
assist
establishing
future
treatment
options
demonstrating
how
neuroinflammation
contributes
illness.
book
chapter
explains
major
contributor
mental
this
topic
significant
study
therapy.
Cells,
Год журнала:
2025,
Номер
14(7), С. 525 - 525
Опубликована: Апрель 1, 2025
Alzheimer’s
disease
(AD)
is
a
multifactorial
neurodegenerative
disorder
characterized
by
metabolic
dysregulation,
oxidative
stress,
amyloid-β
(Aβ)
aggregation,
metal
dyshomeostasis,
and
mitochondrial
dysfunction.
Current
treatments
provide
only
symptomatic
relief,
highlighting
the
need
for
novel
therapeutic
strategies.
This
study
investigates
effects
of
alkaloids
galantamine
(GAL)
lycorine
(LYC)
in
differentiated
SH-SY5Y
neuroblastoma
cells,
an
established
vitro
model
AD,
which
acquire
neuronal
phenotype
upon
differentiation.
Using
untargeted
targeted
NMR-based
metabolomics
combined
with
multivariate
statistical
analysis,
we
analyzed
extracellular
profiles
under
basal
conditions
following
Aβ42
exposure,
both
presence
absence
GAL
LYC.
Our
findings
reveal
distinct
responses
to
Aβ
toxicity,
significant
alterations
pyruvate
glutamine
metabolism.
Both
LYC
contributed
restoration
lysine
homeostasis,
but
had
more
pronounced
effect,
better
sustaining
cellular
energy
balance
function.
Unlike
LYC,
treatment
was
associated
accumulation,
response
between
two
compounds.
These
variations
may
reflect
mechanisms
action,
potentially
influencing
their
roles
counteracting
Aβ-induced
toxicity.
highlights
value
profiling
assessing
neuroprotective
agents
reinforces
potential
natural
this
context.
Neurochemical Research,
Год журнала:
2024,
Номер
49(10), С. 2854 - 2870
Опубликована: Июль 18, 2024
Abstract
This
study
aimed
to
assess
the
impact
of
conditioned
medium
from
epidermal
neural
crest
stem
cells
(EPI-NCSCs-CM)
on
functional
recovery
following
spinal
cord
injury
(SCI),
while
also
exploring
involvement
PI3K-AKT
signaling
pathway
in
regulating
neuronal
apoptosis.
EPI-NCSCs
were
isolated
10-day-old
Sprague-Dawley
rats
and
cultured
for
48
h
obtain
EPI-NCSC-CM.
SHSY-5Y
subjected
with
H
2
O
treatment
induce
Cell
viability
survival
rates
evaluated
using
CCK-8
assay
calcein-AM/PI
staining.
SCI
contusion
model
was
established
adult
recovery,
utilizing
Basso,
Beattie
Bresnahan
(BBB)
scoring
system,
inclined
test,
footprint
observation.
Neurological
restoration
after
analyzed
through
electrophysiological
recordings.
Histological
analysis
included
hematoxylin
eosin
(H&E)
staining
Nissl
evaluate
tissue
organization.
Apoptosis
oxidative
stress
levels
assessed
TUNEL
ROS
detection
methods.
Additionally,
western
blotting
performed
examine
expression
apoptotic
markers
proteins
related
PI3K/AKT
pathway.
EPI-NCSC-CM
significantly
facilitated
histological
by
inhibiting
apoptosis
modulation
Administration
EPI-NCSCs-CM
alleviated
H2O2-induced
neurotoxicity
vitro.
The
use
LY294002,
a
PI3K
inhibitor,
underscored
crucial
role
contributes
ongoing
exploration
molecular
pathways
involved
(SCI)
repair,
focusing
therapeutic
potential
research
findings
indicate
that
exerts
neuroprotective
effect
suppressing
activation
rats.
These
results
highlight
promising
as
strategy
SCI,
emphasizing
significance
mediating
its
beneficial
effects.
Graphical
