Advanced 3D Models of Human Brain Tissue Using Neural Cell Lines: State-of-the-Art and Future Prospects

Cells, Год журнала: 2023, Номер 12(8), С. 1181 - 1181

Опубликована: Апрель 18, 2023

Human-relevant three-dimensional (3D) models of cerebral tissue can be invaluable tools to boost our understanding the cellular mechanisms underlying brain pathophysiology. Nowadays, accessibility, isolation and harvesting human neural cells represents a bottleneck for obtaining reproducible accurate gaining insights in fields oncology, neurodegenerative diseases toxicology. In this scenario, given their low cost, ease culture reproducibility, cell lines constitute key tool developing usable reliable brain. Here, we review most recent advances 3D constructs laden with lines, highlighting advantages limitations possible future applications.

Язык: Английский

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Design, synthesis and bioevaluation of 1,2,4-thiadiazolidine-3,5-dione derivatives as potential GSK-3β inhibitors for the treatment of Alzheimer's disease

Bioorganic Chemistry, Год журнала: 2023, Номер 134, С. 106446 - 106446

Опубликована: Фев. 25, 2023

Язык: Английский

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Implantation of MSC Spheroid-derived 3D Decellularized ECM Enriched with the MSC Secretome Ameliorates Traumatic Brain Injury and Promotes Brain Repair

Biomaterials, Год журнала: 2024, Номер 315, С. 122941 - 122941

Опубликована: Ноя. 1, 2024

Язык: Английский

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Mitochondria transfer as a potential therapeutic mechanism in Alzheimer’s disease-like pathology

Brain Research, Год журнала: 2023, Номер 1819, С. 148544 - 148544

Опубликована: Авг. 22, 2023

Язык: Английский

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Epidermal Neural Crest Stem Cell Conditioned Medium Enhances Spinal Cord Injury Recovery via PI3K/AKT-Mediated Neuronal Apoptosis Suppression

Neurochemical Research, Год журнала: 2024, Номер 49(10), С. 2854 - 2870

Опубликована: Июль 18, 2024

Abstract This study aimed to assess the impact of conditioned medium from epidermal neural crest stem cells (EPI-NCSCs-CM) on functional recovery following spinal cord injury (SCI), while also exploring involvement PI3K-AKT signaling pathway in regulating neuronal apoptosis. EPI-NCSCs were isolated 10-day-old Sprague-Dawley rats and cultured for 48 h obtain EPI-NCSC-CM. SHSY-5Y subjected with H 2 O treatment induce Cell viability survival rates evaluated using CCK-8 assay calcein-AM/PI staining. SCI contusion model was established adult recovery, utilizing Basso, Beattie Bresnahan (BBB) scoring system, inclined test, footprint observation. Neurological restoration after analyzed through electrophysiological recordings. Histological analysis included hematoxylin eosin (H&E) staining Nissl evaluate tissue organization. Apoptosis oxidative stress levels assessed TUNEL ROS detection methods. Additionally, western blotting performed examine expression apoptotic markers proteins related PI3K/AKT pathway. EPI-NCSC-CM significantly facilitated histological by inhibiting apoptosis modulation Administration EPI-NCSCs-CM alleviated H2O2-induced neurotoxicity vitro. The use LY294002, a PI3K inhibitor, underscored crucial role contributes ongoing exploration molecular pathways involved (SCI) repair, focusing therapeutic potential research findings indicate that exerts neuroprotective effect suppressing activation rats. These results highlight promising as strategy SCI, emphasizing significance mediating its beneficial effects. Graphical

Язык: Английский

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The role of neuron-like cell lines and primary neuron cell models in unraveling the complexity of neurodegenerative diseases: a comprehensive review

Molecular Biology Reports, Год журнала: 2024, Номер 51(1)

Опубликована: Сен. 28, 2024

Язык: Английский

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Development and In Vitro Evaluation of Biomimetic Injectable Hydrogels from Decellularized Human Nerves for Central Nervous System Regeneration

Materials Today Bio, Год журнала: 2025, Номер 31, С. 101483 - 101483

Опубликована: Янв. 11, 2025

Injuries to the central nervous system (CNS) often lead persistent inflammation and limited regeneration. This study developed a clinically relevant injectable hydrogel derived from decellularized human peripheral nerves, with mechanical properties biomimicking native CNS tissue. Using modified Hudson method, sciatic nerves were decellularized, effectively removing immunogenic cellular debris while retaining extracellular matrix. Two delipidation solvents, dichloromethane: ethanol (2:1 v/v) n-hexane: isopropanol (3:1 v/v), evaluated, former achieving optimal lipid removal better digestion. The resulting solution was crosslinked genipin, forming an (iHPN) that gelled within 12 min at 37 °C exhibited stiffness of approximately 400 Pa. Human astrocytes, microglial cell clone 3 (HMC3), mouse RAW 264.7 macrophages cultured individually iHPN, lipopolysaccharide (LPS) added mimic following injury. Compared LPS-activated cells on tissue culture plates (TCP), astrocytes iHPN maintained quiescent state, as evidenced by reduced GFAP expression IL-1β secretion. HMC3 in displayed anti-inflammatory phenotype, shown increased CD206 decreased CD86/CD68 expression, along higher IL-4 lower TNF-α/IL-1β SH-SY5Y neuroblastoma viability improved neuronal differentiation compared TCP. brain neurons had TCP or collagen hydrogels. Overall, is novel has potential for minimally invasive applications, such carrier drug delivery and/or biomaterial support axonal growth.

Язык: Английский

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Toward the Treatment of Glioblastoma Tumors Using Photoactivated Chemotherapy: In Vitro Evaluation of Efficacy and Safety

ACS Pharmacology & Translational Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 30, 2025

Glioblastoma multiforme (GBM) is highly aggressive, necessitating new therapies. Photoactivated chemotherapy (PACT) offers a promising approach by activating prodrugs with visible light at the tumor site. This study evaluated anticancer activity of ruthenium-based PACT compounds in U-87MG glioblastoma cells and their safety SH-SY5Y neuron-like cells. The compound [3](PF6)2 showed light-activated effects cells, while [1](PF6)2 was inactive, [2](PF6)2 nonactivated. Interestingly, increased cell proliferation, similar to donepezil, without causing death. Increased Ca2+ uptake observed, possibly via interaction AMPA receptor, as suggested docking studies. These findings suggest may serve potential treatments for GBM, effectively attacking cancer preserving healthy neuronal

Язык: Английский

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Corticosteroid‐regulated gene transcription in SH‐SY5Y‐derived neurons: Insights into the mineralocorticoid and glucocorticoid receptor‐mediated response

Journal of Neuroendocrinology, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

Abstract Post‐traumatic stress disorder (PTSD) and major depressive (MDD) are debilitating stress‐related psychiatric disorders that can develop following exposure to traumatic events or chronic in some individuals. The neurobiological processes leading disease remain largely unknown. Among others, these characterized by a dysregulated hypothalamic–pituitary–adrenal axis, which is regulated the glucocorticoid receptor (GR) mineralocorticoid (MR). This leads altered downstream corticosteroid‐induced gene expression. In vitro models promising tools investigate specific underpinnings of response brain. Here, we investigated suitability SH‐SY5Y‐derived neurons as cost‐efficient system study role GR MR neuronal response. were characterized, exposed corticosteroids, analyzed on transcriptomic proteomic levels. We show (i) express sufficient seemingly functional allow transcription, (ii) three corticosteroids cortisol, dexamethasone, aldosterone, induced similar effects, (iii) antagonist spironolactone mildly attenuated effects dexamethasone FKBP5 , DUSP1 SUPV3L1 . Mifepristone did not significantly alter effect aldosterone. (iv) Integrating alterations corticosteroid‐exposed with those iPSC‐derived showed concordant two systems. To determine translational validity, compared expression transcriptome postmortem brain samples from individuals PTSD MDD, yielding stronger negative correlations corticosteroid signatures than MDD signatures. Upon further refinement validation, may serve simplistic tool implicated molecular networks around MR. Strengthening our insight into receptors' functions improves understanding commonly such MDD.

Язык: Английский

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The Role and Importance of Neuroinflammation in Biopsychiatry

IntechOpen eBooks, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

Neuroinflammation is an inflammatory response that affects the central nervous system. This process involves activation of immune cells like microglia and astrocytes, as well production chemicals cytokines chemokines. can be caused by a variety circumstances, including trauma, infection, autoimmune illnesses, environmental factors, any stress scenario, neurodegenerative diseases. thought to connected with psychiatric disorders. These illnesses include depression, anxiety disorders, schizophrenia, bipolar disorder. Research in biological neuropsychiatry assist establishing future treatment options demonstrating how neuroinflammation contributes illness. book chapter explains major contributor mental this topic significant study therapy.

Язык: Английский

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