Comprehensive Tools of Alkaloid/Volatile Compounds–Metabolomics and DNA Profiles: Bioassay-Role-Guided Differentiation Process of Six Annona sp. Grown in Egypt as Anticancer Therapy

Pharmaceuticals, Год журнала: 2024, Номер 17(1), С. 103 - 103

Опубликована: Янв. 11, 2024

Trees of the Annona species that grow in tropics and subtropics contain compounds are highly valuable for pharmacological research medication development have anticancer, antioxidant, migratory properties. Metabolomics was used to functionally characterize natural products distinguish differences between varieties. Natural therefore bioactive-marked respected field drug innovation. Our study aimed evaluate interrelationships among six species. By utilizing Start Codon Targeted (SCoT) Inter Simple Sequence Repeat (ISSR) primers DNA fingerprinting, we discovered polymorphism percentages 45.16 35.29%, respectively. The comparison profiles 78 distinct volatile oil accomplished through utilization GC-MS-based plant metabolomics. Additionally, differentiation process 74 characterized alkaloid compound metabolomics conducted a structural analysis using HPLC-ESI-MSn UPLC-HESI-MS/MS, antiproliferative activities were assessed on five vitro cell lines. High-throughput, low-sensitivity LC/MS-based has facilitated comprehensive examinations alterations secondary metabolites bioassay-guided processes. This been by employing twenty-four extracts derived from species, which subjected evaluation. primary objective this evaluation investigate IC50 profile as well antioxidant migration activities. It should be noted, however, these investigations exclusively most potent extracts. These thoroughly examined both HepG2 Caco lines elucidate their potential anticancer effects. In tests cultures showed significant concentration cytotoxic effect all (HepG2, HCT, Caco, Mcf-7, T47D) treated with essential samples at exposure time (48 h). Therefore, they remarkable activity simultaneous total, 50% 80% A. muricata extract, extract highest activity, demonstrated dose-dependent inhibition migration. strong metastatic cells 48 h after treatment scraping sheet, best reduction caused reticulata extract. Also, showing value stopping metastasis invasion various cancer lines, making them an interesting topic further research.

Язык: Английский

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Role of rich phenolics and betanin profiles from Opuntia ficus-indica fruits in the prevention of diabetic complications using metabolomics study

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Фев. 17, 2025

Язык: Английский

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Development and in vitro/in vivo evaluation of a nanosponge formulation loaded with Boswellia carterii oil extracts for the enhanced anti-inflammatory activity for the management of respiratory allergies

Journal of Pharmaceutical Investigation, Год журнала: 2024, Номер 54(5), С. 643 - 665

Опубликована: Апрель 8, 2024

Abstract Purpose In several nations, it has been known that Boswellia carterii (BC) plants have a long history of usage as an anti-inflammatory medicine. Our current study deals with the two different ways extraction BC volatile oil and fixed main constituents (Octyl acetate, 46.46% β-Boswellic acid, 10.21%, respectively), preparation, evaluation Nano sponges loaded either dexamethasone (DEX) or B. volatile/fixed mixture. Methods were created utilizing ultrasound-assisted synthesis method. Using spectrophotometry, entrapment efficiency (EE%) drugs in was considered. Several techniques used to characterize sponges. Results EE% inside ranged from 99.15 ± 3.50 100% 4.76%. Particle size 59.9 26.3 229.2 30.23 nm. Drugs released bi-phasically according Korsmeyer–Peppas model. The activity mixture Dexa salt their formulations (D4 O1) treatment respiratory allergy evaluated rat model mimicking chronic allergic diseases. Histopathologic examinations measurement Intracellular adhesion molecule-1 (ICAM-1), Leukotriene B 4 (LTB ) Interleukin β4 (ILβ4) levels exposed that, significantly lowered inflammatory biomarkers treated rats exhibited improved histopathologic profiles when compared positive control group. Conclusion its sponge formulation O1 well D4 had promising therapeutic effects on upper lower conditions.

Язык: Английский

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Appraisal terpenoids rich Boswellia carterri ethyl acetate extract in binary cyclodextrin oligomer nano complex for improving respiratory distress

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июль 22, 2024

Boswellia carterii (BC) resins plants have a long historical background as treatment for inflammation, indicated by information originating from multiple countries. Twenty-seven diterpenoids been identified in ethyl acetate and total methanol BC, comprising seventeen boscartins of the cembrane-type ten boscartols prenylaromadendrane-type diterpenoids. Moreover, twenty-one known triterpenoids also found, encompassing nine tirucallane-type, six ursane-type, four oleanane-type, two lupane-type. The hold significant position pharmaceutical chemistry related industries due to their captivating biological characteristics promising pharmacological potentials. Extraction creation assessment nano sponges loaded with either B. plant extract or DEX, are subjects our current investigation. With use ultrasound-assisted synthesis, were produced. entrapment efficiency (EE%) medications was examined using spectrophotometry. Nano characterized number methods. Within sponges, EE% medicines varied between 98.52 ± 0.07 99.64 1.40%. sponges' particle sizes 105.9 15.9 166.8 26.3 nm. Drugs released Korsmeyer-Peppas concept. In respiratory distressed rats, effects BC extract, DEX salt formulations (D1, D5, P1 P1), tested. Treatment significantly reduced ICAM-1, LTB4, ILβ 4 levels improved histopathologic profiles, when compared positive control group. its sponge formulation showed therapeutic effects. effect may be synergism both formulation. This achieved blocking ICAM-1 LTB4 pathways, therefore counteracting talc powder.

Язык: Английский

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A comprehensive tool in recycling plant-waste of Gossypium barbadense L agricultural and industrial waste extracts containing gossypin and gossypol: hepatoprotective, anti-inflammatory and antioxidant effects

Plant Methods, Год журнала: 2024, Номер 20(1)

Опубликована: Апрель 17, 2024

Abstract Improper management of agricultural and industrial cotton wastes causes environmental pollution worsens the climate change challenge. Green recycling could contribute to a circular economy. One economic values lies in their bioactive components. Two types wastes—agricultural industrial—of species Gossypium barbadense L. Giza 95 were targeted current study, aiming maximize medicinal value investigate anti-inflammatory, hepatoprotective, antioxidant activities phytochemical extracts. Phytochemical extraction was performed using different solvents extraction. An anti-inflammatory effect tested carrageenan-induced acute edema rat paw model. A carbon tetrachloride chronic model liver injury used for assessment hepatoprotective potential. Liver enzymes (AST ALT), oxidative stress markers (MDA GSH), inflammatory biomarkers (C-reactive protein), histopathological features investigated. As result, ethyl acetate proved be solvent best choice extract gossypin polyphenolics, where extracted amount reached 14,826.2 µg/g, followed by butanol (8751.4 µg/g extract). The chloroform (CHCL 3 ) fraction showed highest amounts gossypol (190.7 extract), petroleum ether. Cotton waste’s composition analysis wide range components, including 33 metabolites such as gossypetin, other that possess therapeutic effects. Both waste extracts superior effects comparison All (ethyl acetate, chloroform, waste) proper activities. Graphical

Язык: Английский

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A novel therapeutic approach to modulate the inflammatory cascade: A timely exogenous local inflammatory response attenuates the sepsis-induced cytokine storm

Cytokine, Год журнала: 2024, Номер 176, С. 156533 - 156533

Опубликована: Фев. 9, 2024

Язык: Английский

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The wide spectrum anti-inflammatory activity of andrographolide in comparison to NSAIDs: A promising therapeutic compound against the cytokine storm

PLoS ONE, Год журнала: 2024, Номер 19(7), С. e0299965 - e0299965

Опубликована: Июль 17, 2024

The challenges of the COVID-19 pandemic have highlighted an increasing clinical demand for safe and effective treatment options against overzealous immune defence response, also known as “cytokine storm”. Andrographolide is a naturally derived bioactive compound with promising anti-inflammatory activity in many studies. However, its cytokine-inhibiting activity, direct comparison to commonly used nonsteroidal drugs (NSAIDs), has not been extensively investigated existing literature. activities andrographolide common NSAIDs, such diclofenac, aspirin, paracetamol ibuprofen were measured on lipopolysaccharide (LPS) interferon-γ induced RAW264.7 cells. levels PGE2, nitric oxide (NO), TNF-α & LPS-induced release pro-inflammatory cytokines differentiated human macrophage THP-1 cells concentrations aforementioned NSAIDs. associated mechanistic pathway was examined NF κ B using flow cytometry endothelial-leukocyte adhesion molecule (ELAM9) (E-selectin) transfected green fluorescent protein (GFP). exhibited broad potent both cell lines by inhibiting IL-6, IFN-γ, which are play key role etiology cytokine storm pathogenesis inflammation. In comparison, tested NSAIDs demonstrated weak or no proinflammatory mediators except where (IC 50 = 8.8 μM, 95% CI 7.4 10.4 μM) comparable that 7.73 6.14 9.73 μM). action downregulation B. wide-spectrum demonstrates therapeutic potential storms alternative

Язык: Английский

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Exploring the interplay between inflammation and male fertility

FEBS Journal, Год журнала: 2024, Номер unknown

Опубликована: Дек. 19, 2024

Male fertility results from a complex interplay of physiological, environmental, and genetic factors. It is conditioned by the properly developed anatomy reproductive system, hormonal regulation balance, between different cell populations that sustain an appropriate functional environment in testes. Unfortunately, mechanisms sustaining male are not flawless their perturbation can lead to infertility. Inflammation one factors contribute In testes, it be brought on varicocele, obesity, gonadal infections, leukocytospermia, physical obstructions or traumas, consumption toxic substances. As result prolonged untreated inflammation, testicular resident cells spermatogenesis suffer DNA damage, lipid protein oxidation, mitochondrial dysfunction consequently leading loss function affected Sertoli (SCs) Leydig (LCs), formation morphologically abnormal dysfunctional sperm lay basis infertility subfertility. This due mainly production secretion pro-inflammatory mediators, including cytokines, chemokines, reactive oxygen species (ROS) local immune (macrophages, lymphocytes T, mast cells) tissue-specific [SCs, LCs, peritubular myoid (PMCs) germ (GCs)]. Depending location, duration, intensity these mediators exert effect elements this review, we discuss most prevalent inflammatory negatively affect describe ways inflammation impair function.

Язык: Английский

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Inflammatory mediators, oxidative stress and genetic disturbance in rheumatoid arthritis rats supported by alfalfa seeds metabolomic constituents via blocking interleukin‐1receptor.

Chemistry & Biodiversity, Год журнала: 2023, Номер 21(2)

Опубликована: Дек. 30, 2023

Rheumatoid arthritis (RA) is an autoimmune disease characterized by aggressive cartilage and bone erosion. This work aimed to evaluate the metabolomic profile of Medicago sativa L. (MS) (alfalfa) seeds explore its therapeutic impact against RA in rats. Arthritis was induced complete Freund's adjuvant (CFA) severity assessed index. Treatment with MS butanol fraction interlukin-1 receptor antagonist (IL-1RA) were evaluated through measuring (IL-1R) type 1 gene expression, beta (IL-1β), oxidative stress markers, C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α), prostaglandin E2 (PGE2), caspase-3 (Cas-3), intracellular adhesion molecule-1 (ICAM-1), DNA fragmentation, chromosomal damage. Total phenolics/ flavonoids content ethyl acetate, crude extract estimated. The major identified compounds Quercetin, Trans-taxifolin, Gallic acid, 7,4'-Dihydroxyflavone, Cinnamic Kudzusaponin SA4, Isorhamnetin 3-O-beta-D-2'',3'',4''-triacetylglucopyranoside, Apigenin, 5,7,4'-Trihydroxy-3'-methoxyflavone, Desmethylxanthohumol, Pantothenic Soyasapogenol E, Malvidin, Helilandin B, Stigmasterol, Wairol. IL-1RA enhanced all biochemical parameters histopathological features ankle joint. In conclusion, Trans-taxifolin isolated for first time from genus Medicago. IL-1 considered as anti-rheumatic agents.

Язык: Английский

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Bio-intelligent plasma-engineered diferuloylmethane/fucoidan/neutrophil lysate/iron oxide nanoclusters for phototherapeutic and magnetotherapeutic with in situ magnetic gelation mitigating inflammatory diseases

Colloids and Surfaces B Biointerfaces, Год журнала: 2024, Номер 243, С. 114054 - 114054

Опубликована: Июль 4, 2024

Язык: Английский

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