Organ-on-a-Chip Applications in Microfluidic Platforms

Micromachines, Год журнала: 2025, Номер 16(2), С. 201 - 201

Опубликована: Фев. 10, 2025

Microfluidic technology plays a crucial role in organ-on-a-chip (OoC) systems by replicating human physiological processes and disease states, significantly advancing biomedical research drug discovery. This article reviews the design fabrication of microfluidic devices. It also explores how these technologies are integrated into OoC platforms to simulate environments, highlighting key principles, technological advances, diverse applications. Through case studies involving simulation multiple organs such as heart, liver, lungs, evaluates impact systems' on screening, toxicity assessment, personalized medicine. In addition, this considers technical challenges, ethical issues, future directions, looks ahead further optimizing functionality biomimetic precision OoCs through innovation, emphasizing its critical promoting medicine treatment strategies.

Язык: Английский

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Assessment of air pollutant O3 pulmonary exposure using a bronchus-on-chip model coupling with atmospheric simulation chamber

Journal of Hazardous Materials, Год журнала: 2025, Номер 486, С. 137106 - 137106

Опубликована: Янв. 5, 2025

Язык: Английский

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Appraisal terpenoids rich Boswellia carterri ethyl acetate extract in binary cyclodextrin oligomer nano complex for improving respiratory distress

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Июль 22, 2024

Boswellia carterii (BC) resins plants have a long historical background as treatment for inflammation, indicated by information originating from multiple countries. Twenty-seven diterpenoids been identified in ethyl acetate and total methanol BC, comprising seventeen boscartins of the cembrane-type ten boscartols prenylaromadendrane-type diterpenoids. Moreover, twenty-one known triterpenoids also found, encompassing nine tirucallane-type, six ursane-type, four oleanane-type, two lupane-type. The hold significant position pharmaceutical chemistry related industries due to their captivating biological characteristics promising pharmacological potentials. Extraction creation assessment nano sponges loaded with either B. plant extract or DEX, are subjects our current investigation. With use ultrasound-assisted synthesis, were produced. entrapment efficiency (EE%) medications was examined using spectrophotometry. Nano characterized number methods. Within sponges, EE% medicines varied between 98.52 ± 0.07 99.64 1.40%. sponges' particle sizes 105.9 15.9 166.8 26.3 nm. Drugs released Korsmeyer-Peppas concept. In respiratory distressed rats, effects BC extract, DEX salt formulations (D1, D5, P1 P1), tested. Treatment significantly reduced ICAM-1, LTB4, ILβ 4 levels improved histopathologic profiles, when compared positive control group. its sponge formulation showed therapeutic effects. effect may be synergism both formulation. This achieved blocking ICAM-1 LTB4 pathways, therefore counteracting talc powder.

Язык: Английский

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Complex in vitro models positioned for impact to drug testing in pharma: a review

Biofabrication, Год журнала: 2024, Номер 16(4), С. 042006 - 042006

Опубликована: Авг. 27, 2024

Abstract Recent years have seen the creation and popularization of various complex in vitro models (CIVMs), such as organoids organs-on-chip, a technology with potential to reduce animal usage pharma while also enhancing our ability create safe efficacious drugs for patients. Public awareness CIVMs has increased, part, due recent passage FDA Modernization Act 2.0. This visibility is expected spur deeper investment adoption models. Thus, end-users model developers alike require framework both understand readiness current enter drug development process, assess upcoming same. review presents selection based on comparative -omics data (which we term model-omics), metrics qualification specific test assays that may support context-of-use (COU) assays. We surveyed existing healthy tissue ten development-critical organs body, provide evaluations suggestions improving model-omics COU each. In whole, this comes from perspective, seeks an evaluation where are poised maximum impact roadmap realizing potential.

Язык: Английский

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On the path to predicting immune responses in the lung: Modeling the pulmonary innate immune system at the air-liquid interface (ALI)

European Journal of Pharmaceutical Sciences, Год журнала: 2023, Номер 191, С. 106596 - 106596

Опубликована: Сен. 26, 2023

Chronic respiratory diseases and infections are among the largest contributors to death globally, many of which still have no cure, including chronic obstructive pulmonary disorder, idiopathic fibrosis, syncytial virus others. Pulmonary therapeutics afford untapped potential for treating lung infection disease through direct delivery site action. However, ability innovate new therapeutic paradigms will rely on modeling human microenvironment key cellular interactions that drive disease. One feature is air-liquid interface (ALI). ALI techniques, using cell-culture inserts, organoids, microfluidics, precision slices (PCLS), rapidly developing; however, one major component these models lacking—innate immune cell populations. Macrophages, neutrophils, dendritic cells, others, represent populations, acting as first responders during or injury. Innate cells respond modulate stromal bridge gap between innate adaptive system, controlling bodies response foreign pathogens debris. In this article, we review current state culture systems with a focus suggest ways build add complexity relevant

Язык: Английский

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Inhalable Mucociliary-On-Chip System Revealing Pulmonary Clearance Dynamics in Nanodrug Delivery

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Янв. 7, 2025

The development of a inhaled nanodrug delivery assessment platform is crucial for advancing treatments chronic lung diseases. Traditional in vitro models and commercial aerosol systems fail to accurately simulate the complex human respiratory patterns mucosal barriers. To address this, we have developed breathing mucociliary-on-a-chip (BMC) platform, which replicates mucociliary clearance dynamics vitro. This allows precise analysis drug deposition penetration, providing critical insights into how liposomes other nanocarriers interact with tissues under various airflow conditions. Our results reveal that penetrate deeper cellular layer high shear stress, both static dynamic airflows distinctly affecting their release rates. BMC integrates inhalation functionality, enabling comprehensive evaluation efficacy. approach highlights importance optimizing inhalable systems, improving nanocarrier design, tailoring dosages strategies. represents significant advancement field delivery, offering more accurate reliable method assessing performance therapies. By detailed understanding interactions tissues, this supports personalized therapies offers promising strategies treating pulmonary diseases development.

Язык: Английский

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In vitro models to study viral-induced asthma exacerbation: a short review for a key issue

Frontiers in Allergy, Год журнала: 2025, Номер 6

Опубликована: Март 28, 2025

Asthma is a heterogenous inflammatory bronchial disease involving complex mechanisms, several pathways, and multiples cell-type networks. Bronchial inflammation associated to asthma consecutive multiple aggressions on epithelium, such as microbiologic, pollutant, antigenic agents, which are responsible for both T2 non-T2 responses further airway remodeling. Because physiopathology involves crosstalk between cell types from different origins (epithelial, mesenchymal, immune cells) numerous cellular effectors, no single and/or representative in vitro model suitable study the overall of this disease. In short review, we present discuss advantages limitations models decipher aspects virus-related exacerbation.

Язык: Английский

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A next-generation system for smoke inhalation integrated with a breathing lung-on-chip to model human lung responses to cigarette exposure

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Май 25, 2025

Continuous exposure to cigarette smoke (CS) significantly contributes the development and progression of chronic obstructive pulmonary disease (COPD) lung cancer. Animal models that inhale nasally have different physiology from humans may not accurately replicate smoke-induced health effects. Furthermore, traditional in vitro fail lung's dynamic mechanical forces realistic inhalation patterns, limiting their relevance preclinical research. Here, we introduce an advanced inhalation-based lung-on-chip system, Flow AX12 (CFAX12), investigate CS-induced cellular responses a physiologically relevant manner. Unlike previous technologies, CFAX12 integrates cyclic stretch with controlled whole-smoke exposure, allowing for more accurate recreation alveolar microenvironment dynamics barrier integrity responses. Using human epithelial cells, microvascular endothelial macrophages mono- co-culture under air-liquid interface (ALI) conditions breathing-like (Str), simulated key features. Our results show CS using induced ~ 60% reduction trans-barrier electrical resistance (TER), increased ROS generation depending on model complexity, 4.5-fold increase IL-8 gene expression, all hallmarks early COPD pathogenesis. These findings underscore damage, inflammation, oxidative stress, which contribute dysfunction progression. Also, provides alternative submerged extract (CSE) treatments, offering VC10 Smoking Robot, ensuring precisely regulated delivery. Additionally, inclusion surfactant reduced IL8 levels by 5 folds. Hence, integrating biological offers robust platform assessing inhaled effects identifying therapeutic targets. It's application drug screening can facilitate discovery compounds preserve integrity, reduce mitigate ultimately bridging gap between regulatory research applications.

Язык: Английский

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How to use an in vitro approach to characterize the toxicity of airborne compounds

Toxicology in Vitro, Год журнала: 2023, Номер 94, С. 105718 - 105718

Опубликована: Окт. 21, 2023

Язык: Английский

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In Vitro Modeling of Idiopathic Pulmonary Fibrosis: Lung-on-a-Chip Systems and Other 3D Cultures

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(21), С. 11751 - 11751

Опубликована: Ноя. 1, 2024

Idiopathic pulmonary fibrosis (IPF) is a lethal disorder characterized by relentless progression of lung that causes respiratory failure and early death. Currently, no curative treatments are available, existing therapies include limited selection antifibrotic agents only slow disease progression. The development novel therapeutics has been hindered understanding the disease's etiology pathogenesis. A significant challenge in developing new IPF lack vitro models accurately replicate crucial microenvironments. In response, three-dimensional (3D) have emerged as powerful tools for replicating organ-level microenvironments seen vivo. This review summarizes state art advanced 3D mimic many physiological pathological processes observed IPF. We begin with brief overview conventional models, such 2D cell cultures animal then explore more focusing on lung-on-a-chip systems. discuss current challenges future research opportunities this field, aiming to advance devices assess effectiveness treatments.

Язык: Английский

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