Quality Study of HuangqiGuizhiWuwu Decoction Based on Ultra Performance Liquid Chromatography Fingerprinting, Chemical Pattern Recognition and Multi‐Component Quantitative Analysis
Separation Science Plus,
Год журнала:
2025,
Номер
8(3)
Опубликована: Фев. 28, 2025
ABSTRACT
HuangqiGuizhiWuwu
decoction
(HGWD)
possesses
a
complex
and
diverse
chemical
composition,
rendering
the
identification
of
its
components
challenging.
In
this
study,
UPLC
fingerprinting
profile
HGWD
was
elucidated
method
determining
multiple
in
proposed.
Common
peaks
were
selected
from
12
batches
HGWD,
Chinese
herbal
chromatographic
fingerprint
similarity
evaluation
system
(2012)
employed
for
analyses.
The
corresponding
to
common
identified
using
UPLC‐Q‐TOF‐MS.
Chemical
analyses
conducted
evaluate
quality
different
identify
primary
components.
similarities
profiles
all
>
0.98,
with
15
confirmed
divided
into
three
categories,
OPLS‐DA
used
cinnamic
acid
cinnamaldehyde
as
characteristic
that
caused
differences.
five
which
included
albiflorin,
paeoniflorin,
calycosin‐7‐O‐β‐D‐glucoside,
acid,
cinnamaldehyde,
exhibited
good
linear
relationships
average
recovery
rates
relative
standard
deviations.
This
study
established
proposed
simple,
feasible,
sensitive,
reproducible
content
determination
method.
Moreover,
our
findings
provide
robust
foundation
standardization
HGWD.
Язык: Английский
Screening of potential antioxidant bioactive Q-markers of paeoniae radix rubra based on an integrated multimodal strategy
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Авг. 15, 2024
Background
Paeoniae
Radix
Rubra
(PRR)
has
been
used
widely
to
promote
blood
circulation
and
eliminate
stasis
in
China
clinical
practice
owing
its
extensive
pharmacological
effects.
However,
the
“quality
markers”
(Q-markers)
of
antioxidant
effects
remains
unknown.
Object
To
explore
Q-markers
activity
based
on
multiple
strategies,
which
would
provide
reference
for
quality
evaluation
PRR
specific
pharmacodynamic-oriented.
Methods
Firstly,
“fingerprint”
profiles
15
batches
were
acquired
identified
by
ultrahigh
performance
liquid
chromatography-quadrupole
time-of-flight
tandem
mass
spectrometry
(UHPLC-Q-TOF
MS/MS)
common
peaks
extracted.
Meanwhile,
MTT
assay
was
evaluate
effect
H
2
O
-induced
oxidative
stress
HT-22
cells.
The
investigated
simultaneously
superoxide
dismutase
(SOD),
glutathione
(GSH),
malondialdehyde
(MDA)
commercial
kits.
relationship
between
indexes
constructed
grey
relational
analysis
(GRA)
partial
least
squares-discriminant
(PLS-DA)
identification
preselected
Q-markers.
Secondly,
experimental
verification
conducted
investigate
protective
preliminary
components
cells
undergoing
stress.
Finally,
further
validation
effectiveness
Q-markers,
network
pharmacology
applied
potential
targets,
molecular
docking
technology
value
binding
ability
active
targets.
Results
Thirty-seven
from
qualitatively
UHPLC-Q-TOF
MS/MS.
showed
that
could
reduce
damage
induced
upon
according
index
MDA,
SOD
GSH.
Eight
screened
spectrum-effect
correlation
analysis:
paeoniflorin,
galloylpaeoniflorin,
albiflorin,
1,2,3,4,6-o-pentagalloylglucose,
benzoylpaeoniflorin,
pinocembrin,
oleanic
acid,
isorhamnetin-3-o-nehesperidine.
Each
these
significant
protections
cellular
(
P
<
0.05).
Interleukin-6
(IL-6),
protein
kinase
B
(AKT1),
tumor
necrosis
factor
(TNF)
predicted
be
major
targets
PRR,
good
presented
each
target
as
a
whole.
Conclusion
an
integrated
multimodal
strategy.
Язык: Английский
Jiawei Kongsheng Zhenzhong Pill (JKZP) Alleviates Chronic Cerebral Hypoperfusion‐Induced Hippocampal Synaptic Damage via S100A10/tPA/BDNF Pathway
Brain and Behavior,
Год журнала:
2025,
Номер
15(2)
Опубликована: Фев. 1, 2025
ABSTRACT
Aims
To
evaluate
the
effects
of
Jiawei
Kongsheng
Zhenzhong
Pill
(JKZP)
on
rats
with
ischemic
mild
cognitive
impairment
(MCI)
and
investigate
underlying
mechanisms.
Methods
The
components
JKZP
were
analyzed
using
Q‐Orbitrap
high‐resolution
mass
spectrometry
(HRMS).
MCI
rat
model
was
prepared
through
gradual
bilateral
common
carotid
artery
occlusion
(BCCAO).
function,
hippocampal
pathological
lesions,
dendritic
spine
damage,
synapse‐related,
S100
calcium‐binding
protein
A10
(S100A10)/tissue‐type
plasminogen
activator
(tPA)/brain‐derived
neurotrophic
factor
(BDNF)
pathway‐associated
molecules
alterations
measured.
Primary
hippocampus
neurons
subjected
to
oxygen‐glucose
deprivation/reperfusion
(OGD/R)
injury,
JKZP‐containing
serum
utilized
for
treatment.
Lentiviral‐infected
constructed
S100A10
knockdown
RNAi
technology
whether
exerted
its
anti‐MCI
via
S100A10/tPA/BDNF
pathway.
Results
A
total
64
major
components,
including
β‐asarone,
ferulic
acid,
loganin,
senkyunolide
H,
cryptotanshinone,
identified
by
HRMS
technology.
had
a
notable
impact
enhancing
abilities
MCI.
reduced
damage
CA1
region
neuron
synaptic
structure,
reversed
decrease
in
spines,
increased
expressions
synapse‐associated
proteins
such
as
synaptophysin
(SYN),
growth‐associated
43
(GAP43),
postsynaptic
density
95
(PSD95).
Furthermore,
treatment
dramatically
ratio
BDNF
(proBDNF)/mature
(mBDNF)
activating
S100A10/tPA,
which
confirmed
primary
vitro.
Moreover,
sh‐S100A10
tremendously
mitigated
inhibitory
action
OGD/R‐mediated
synapse
decreased
activity
tPA,
thus
improved
downstream
pathway
targets’
ratio,
proBDNF/mBDNF.
Conclusions
These
results
manifested
that
promoted
neurological
recovery
after
chronic
cerebral
ischemia
alleviating
pathway,
thereby
providing
novel
perspective
solid
foundation
against
Язык: Английский
Angelicin ameliorated ulcerative colitis through activating HDAC1-derived HIF-1α acetylation
Haifan Liu,
Dunfang Wang,
Xue Feng
и другие.
Phytomedicine,
Год журнала:
2025,
Номер
140, С. 156546 - 156546
Опубликована: Фев. 19, 2025
Язык: Английский
Clinical application, potential pharmacological targets and quality marker prediction of a TCM formulation used (Shenling Baizhu San) in the treatment of respiratory diseases
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Май 29, 2025
Shenling
Baizhu
San
(SLBZS)
is
a
formulation
of
traditional
Chinese
medicine
(TCM)
recorded
in
the
Song
Dynasty
medical
book
Taiping
Huimin
Heji
Jufang
(AD
1078–1085).
It
comprises
eleven
herbs:
Ginseng
Radix
Et
Rhizoma,
Atractylodis
Macrocephalae
Poria,
Dioscoreae
Nelumbinis
Semen,
Coicis
Lablab
Semen
Album,
Amomi
Fructus,
Platycodonis
Radix,
Glycyrrhizae
and
Jujubae
Fructus.
SLBZS
has
been
employed
for
over
900
years
treatment
pulmonary
gastrointestinal
disorders
because
its
qualities
that
enhance
spleen
function,
tonify
lungs,
supplement
qi,
mitigate
diarrhoea.
This
study
meticulously
examined
synthesised
clinical
relevance
pharmacological
mechanisms
SLBZS,
concentrating
on
respiratory
diseases,
response
to
increasing
volume
data
about
SLBZS.
Meanwhile,
according
five
principles
Q-marker
determination,
including
quality
transmission
traceability,
metabolites
specificity,
formula
compatibility
environment,
association
between
effectiveness,
measurability,
potential
markers
(Q-markers)
diseases
were
predicted.
will
provide
additional
research,
clarify
mechanisms,
set
control
criteria
diseases.
Язык: Английский
Identification of chemical composition in Gnetum montanum extract and plasma components after oral administration in cynomolgus monkey by UPLC-Q-TOF-MS and its anti-tumor active components analysis
Xianglong Pan,
Xiaotao Hou,
Fan Zhang
и другие.
Journal of Pharmaceutical and Biomedical Analysis,
Год журнала:
2024,
Номер
249, С. 116347 - 116347
Опубликована: Июль 9, 2024
Gnetum
montanum
Markgr.
(Gnetaceae)
is
a
commonly
used
traditional
herbal
medicine
among
the
Yao
ethnic
group,
with
potential
effects
in
preventing
and
treating
tumors.
However,
substance
basis
of
its
anti-tumor
properties
remains
unclear.
This
study
utilized
ultra-performance
liquid
chromatography-quadrupole
time-of-flight
mass
spectrometry
(UPLC-Q-TOF-MS)
to
identify
chemical
components
G.
extract
(GME)
absorbed
prototypes
cynomolgus
monkey
plasma
after
oral
administration.
A
total
57
compounds
were
detected
GME,
14
positive
ion
mode
43
negative
mode.
In
plasma,
17
identified,
3
Subsequently,
we
high
content
screening
technology
investigate
GME
on
colon
cancer,
lung
breast
gastric
liver
esophageal
cancer.
We
found
that
exhibited
significant
proliferation
inhibition
cancer
cells
SW480,
an
IC
Язык: Английский