6-cyanodopamine as an Endogenous Modulator of Heart Chronotropism and Inotropism DOI Creative Commons

José Britto‐Júnior,

Antonio Tiago Lima,

Denis Lima Oliveira

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 26, 2024

Abstract Purpose- Rat isolated atria and ventricles releases endothelium-derived 6-nitrodopamine this novel catecholamine induces a potent endogenous positive chronotropic inotropic response. 6-Cyanodopamine is released from rabbit ventricles, however it not known whether has any action on the heart. Therefore, was investigated rat release 6-cyanodopamine its Methods- Basal of assessed by LC-MS/MS tyrosine hydroxylase both immunohistochemistry fluorescence in situ hybridization. Chronotropic effects were evaluated in Langendorff’s preparation, respectively. Results- presented basal 6-cyanodopamine, which unaffected pre-treatment with tetrodotoxin. Immunohistochemistry hybridization identified expression endothelium cardiomyocytes. at 10 100 pM induced increases atrial rate, maintained even 30min after preparation washed. In 1min infusion (10 100pM) significantly increased heart frequency, LVDP, dP/dt(max). Bolus injection noradrenaline (1pmol) had no effect LVDP dPdt(max). Infusion (0.01pM) dP/dt(max) when injected end infusion. Conclusion- The results indicate that mediator chronotropism inotropism It potential therapeutic failure may be useful as biomarker pathophysiological processes.

Язык: Английский

6‐Nitrodopamine is an endogenous mediator of rat seminal vesicles contractility DOI Open Access
Vivian Fuguhara,

Gabriel Augusto Oliveira Stocco,

José Britto‐Júnior

и другие.

Andrology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 8, 2025

Abstract Background 6‐Nitrodopamine (6‐ND) released from rat vas deferens acts an endogenous modulator of contractility. Objectives To investigate whether isolated seminal vesicles (RISV) releases 6‐ND, the mechanisms involved in release, and modulatory role 6‐ND on tissue Methods Rat were removed placed Krebs–Henseleit's solution at 37°C for 30 min, aliquot was used to analyze concentrations dopamine, noradrenaline, adrenaline by liquid chromatography with tandem mass spectrometry (LC–MS/MS). The effect mechanical removal epithelium effects pre‐incubation RISV strips N ω ‐nitro‐L‐arginine methyl ester (L‐NAME; combination or not L‐arginine), tetrodotoxin (TTX), GKT137831, hydrogen peroxide (H 2 O ) release evaluated. For functional studies, mounted organ bath tied isometric force transducer. expressions tyrosine hydroxylase endothelial nitric oxide synthase (eNOS, type III NOS) investigated immunohistochemistry and/or fluorescence situ hybridization (FISH). Results major catecholamine compared adrenaline, dopamine. Epithelium significantly reduced noradrenaline In obtained animals chronically treated L‐NAME, reduced. Pre‐incubation L‐NAME which partly restored co‐incubation L‐arginine. TTX had no any catecholamine, whereas GKT137831 H increased release. All catecholamines produced concentration‐dependent contractions, but approximately 30× less potent than others. (0.1 nM) potentiated noradrenaline‐, adrenaline‐, dopamine‐induced such potentiations inhibited TTX. Immunohistochemistry FISH assays tissues identified epithelial cells eNOS expression both cells. Discussion conclusion This is first demonstration that epithelial‐derived modulates vesicle

Язык: Английский

Процитировано

0
6-Cyanodopamine as an endogenous modulator of heart chronotropism and inotropism DOI

José Britto‐Júnior,

Antonio Tiago Lima,

Denis Lima Oliveira

и другие.

Life Sciences, Год журнала: 2025, Номер unknown, С. 123504 - 123504

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Cardiovascular effects of 6-nitrodopamine, adrenaline, noradrenaline, and dopamine in normotensive and hypertensive rats DOI Creative Commons
Vivian Fuguhara, Mariana Gonçalves de Oliveira,

Carlos Alberto Aguiar da Silva

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Май 20, 2025

6-Nitrodopamine (6-ND) has been extensively investigated using in vitro protocols, especially the cardiovascular system. Despite established more potent positive chronotropic and inotropic effects, comparison with classical catecholamines, adrenaline (ADR), noradrenaline (NA) dopamine (DA), effects vivo models are not determined yet. Here, we acute on heart rate (HR) mean arterial blood pressure (MABP) normotensive hypertensive rats by 6-ND. Adult male Wistar were randomly divided into two groups: one received regular filtered water other a chronic Nω-nitro-L-arginine methyl ester (L-NAME) treatment (20mg/day/rat, 4weeks). Thereafter, animals anesthetized, HR MABP monitored through femoral artery, vein was used to administer bolus injections of 6-ND, ADR, NA DA. Moreover, novel 6-cyanodopamine (6-CYANO), 6nitroadrenaline (6-NADR), 6-nitrodopa (6-NDOPA) assess their effect monoamine oxidase (MAO) activity. All four drugs significantly increased control animals; 6-ND than catecholamines its abolished L-NAME treatment. Furthermore, cell-free assay, able partially inhibit MAO-A (<30% at 1 mM) MAO-B (<40% 10 μM); 6-NDOPA 6-CYANO (1 inhibited MAO-A, around 40%, respectively. ADR produced inhibition <20%, 100μM. These results clearly demonstrate that is most endogenous agent yet described, independent MAO inhibition.

Язык: Английский

Процитировано

0
6-cyanodopamine as an Endogenous Modulator of Heart Chronotropism and Inotropism DOI Creative Commons

José Britto‐Júnior,

Antonio Tiago Lima,

Denis Lima Oliveira

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 26, 2024

Abstract Purpose- Rat isolated atria and ventricles releases endothelium-derived 6-nitrodopamine this novel catecholamine induces a potent endogenous positive chronotropic inotropic response. 6-Cyanodopamine is released from rabbit ventricles, however it not known whether has any action on the heart. Therefore, was investigated rat release 6-cyanodopamine its Methods- Basal of assessed by LC-MS/MS tyrosine hydroxylase both immunohistochemistry fluorescence in situ hybridization. Chronotropic effects were evaluated in Langendorff’s preparation, respectively. Results- presented basal 6-cyanodopamine, which unaffected pre-treatment with tetrodotoxin. Immunohistochemistry hybridization identified expression endothelium cardiomyocytes. at 10 100 pM induced increases atrial rate, maintained even 30min after preparation washed. In 1min infusion (10 100pM) significantly increased heart frequency, LVDP, dP/dt(max). Bolus injection noradrenaline (1pmol) had no effect LVDP dPdt(max). Infusion (0.01pM) dP/dt(max) when injected end infusion. Conclusion- The results indicate that mediator chronotropism inotropism It potential therapeutic failure may be useful as biomarker pathophysiological processes.

Язык: Английский

Процитировано

0