6-cyanodopamine as an Endogenous Modulator of Heart Chronotropism and Inotropism DOI Creative Commons

José Britto‐Júnior,

Antonio Tiago Lima,

Denis Lima Oliveira

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

Abstract Purpose- Rat isolated atria and ventricles releases endothelium-derived 6-nitrodopamine this novel catecholamine induces a potent endogenous positive chronotropic inotropic response. 6-Cyanodopamine is released from rabbit ventricles, however it not known whether has any action on the heart. Therefore, was investigated rat release 6-cyanodopamine its Methods- Basal of assessed by LC-MS/MS tyrosine hydroxylase both immunohistochemistry fluorescence in situ hybridization. Chronotropic effects were evaluated in Langendorff’s preparation, respectively. Results- presented basal 6-cyanodopamine, which unaffected pre-treatment with tetrodotoxin. Immunohistochemistry hybridization identified expression endothelium cardiomyocytes. at 10 100 pM induced increases atrial rate, maintained even 30min after preparation washed. In 1min infusion (10 100pM) significantly increased heart frequency, LVDP, dP/dt(max). Bolus injection noradrenaline (1pmol) had no effect LVDP dPdt(max). Infusion (0.01pM) dP/dt(max) when injected end infusion. Conclusion- The results indicate that mediator chronotropism inotropism It potential therapeutic failure may be useful as biomarker pathophysiological processes.

Language: Английский

6‐Nitrodopamine is an endogenous mediator of rat seminal vesicles contractility DOI Open Access
Vivian Fuguhara,

Gabriel Augusto Oliveira Stocco,

José Britto‐Júnior

et al.

Andrology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Abstract Background 6‐Nitrodopamine (6‐ND) released from rat vas deferens acts an endogenous modulator of contractility. Objectives To investigate whether isolated seminal vesicles (RISV) releases 6‐ND, the mechanisms involved in release, and modulatory role 6‐ND on tissue Methods Rat were removed placed Krebs–Henseleit's solution at 37°C for 30 min, aliquot was used to analyze concentrations dopamine, noradrenaline, adrenaline by liquid chromatography with tandem mass spectrometry (LC–MS/MS). The effect mechanical removal epithelium effects pre‐incubation RISV strips N ω ‐nitro‐L‐arginine methyl ester (L‐NAME; combination or not L‐arginine), tetrodotoxin (TTX), GKT137831, hydrogen peroxide (H 2 O ) release evaluated. For functional studies, mounted organ bath tied isometric force transducer. expressions tyrosine hydroxylase endothelial nitric oxide synthase (eNOS, type III NOS) investigated immunohistochemistry and/or fluorescence situ hybridization (FISH). Results major catecholamine compared adrenaline, dopamine. Epithelium significantly reduced noradrenaline In obtained animals chronically treated L‐NAME, reduced. Pre‐incubation L‐NAME which partly restored co‐incubation L‐arginine. TTX had no any catecholamine, whereas GKT137831 H increased release. All catecholamines produced concentration‐dependent contractions, but approximately 30× less potent than others. (0.1 nM) potentiated noradrenaline‐, adrenaline‐, dopamine‐induced such potentiations inhibited TTX. Immunohistochemistry FISH assays tissues identified epithelial cells eNOS expression both cells. Discussion conclusion This is first demonstration that epithelial‐derived modulates vesicle

Language: Английский

Citations

0
6-Cyanodopamine as an endogenous modulator of heart chronotropism and inotropism DOI

José Britto‐Júnior,

Antonio Tiago Lima,

Denis Lima Oliveira

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 123504 - 123504

Published: Feb. 1, 2025

Language: Английский

Citations

0
6-cyanodopamine as an Endogenous Modulator of Heart Chronotropism and Inotropism DOI Creative Commons

José Britto‐Júnior,

Antonio Tiago Lima,

Denis Lima Oliveira

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

Abstract Purpose- Rat isolated atria and ventricles releases endothelium-derived 6-nitrodopamine this novel catecholamine induces a potent endogenous positive chronotropic inotropic response. 6-Cyanodopamine is released from rabbit ventricles, however it not known whether has any action on the heart. Therefore, was investigated rat release 6-cyanodopamine its Methods- Basal of assessed by LC-MS/MS tyrosine hydroxylase both immunohistochemistry fluorescence in situ hybridization. Chronotropic effects were evaluated in Langendorff’s preparation, respectively. Results- presented basal 6-cyanodopamine, which unaffected pre-treatment with tetrodotoxin. Immunohistochemistry hybridization identified expression endothelium cardiomyocytes. at 10 100 pM induced increases atrial rate, maintained even 30min after preparation washed. In 1min infusion (10 100pM) significantly increased heart frequency, LVDP, dP/dt(max). Bolus injection noradrenaline (1pmol) had no effect LVDP dPdt(max). Infusion (0.01pM) dP/dt(max) when injected end infusion. Conclusion- The results indicate that mediator chronotropism inotropism It potential therapeutic failure may be useful as biomarker pathophysiological processes.

Language: Английский

Citations

0