Neuroprotective effects of Hibiscus sabdariffa var. altissima on cerebral ischemia‒Reperfusion injury in rats DOI Creative Commons
Adjia Hamadjida,

Saida Nkuketgnigni Njemguie,

Rigobert Espoir Ayissi Mbomo

и другие.

Pharmacological Research - Modern Chinese Medicine, Год журнала: 2024, Номер 12, С. 100485 - 100485

Опубликована: Авг. 2, 2024

Hibiscus sabdariffa var. altissima, which is known for its high-quality fiber, commonly used in Traditional Chinese Medicine (TCM) diuretic, choleretic, analgesic, antitussive, and hypotensive effects. Furthermore, the flowers have been to treat diabetes, hypertension, atherosclerosis, obesity. However, there are no studies assessing neuroprotective effects on cerebral ischemia, unlike variety Therefore, this study was aimed investigate potential efficacy of altissima extract (HAS) underlying mechanism oxidative stress neuroinflammation during ischemia-reperfusion (I/R) injury. A model ischemia established male Wistar rats through middle artery occlusion (MCAO) reperfusion. Rats were randomly divided into sham, IR, IR + HSA100, HSA200, HSA400 Eda groups treated 14 consecutive days. The neurological deficit score cylinder test performed assess impairment. Oxidative determined by measuring levels malondialdehyde (MDA) antioxidant markers such as reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) measured evaluate activities. In addition, inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), also determined. Our results demonstrated that HSA significantly ameliorated impairment volume brain infarct. decreased MDA enhanced activities GSH, SOD CAT tissues. expression proinflammatory cytokines TNF-α, IL-1β IL-6 serum. These findings exhibited a effect against I/R injury, possibly improving attenuating responses. could be therapeutic option ischemic injuries.

Язык: Английский

Dysfunctional mitochondria in age-related neurodegeneration: Utility of melatonin as an antioxidant treatment DOI Creative Commons
Russel J. Reíter, Ramaswamy Sharma, Walter Manucha

и другие.

Ageing Research Reviews, Год журнала: 2024, Номер 101, С. 102480 - 102480

Опубликована: Сен. 3, 2024

Mitochondria functionally degrade as neurons age. Degenerative changes cause inefficient oxidative phosphorylation (OXPHOS) and elevated electron leakage from the transport chain (ETC) promoting increased intramitochondrial generation of damaging reactive oxygen nitrogen species (ROS RNS). The associated progressive accumulation molecular damage causes an increasingly rapid decline in mitochondrial physiology contributing to aging. Melatonin, a multifunctional free radical scavenger indirect antioxidant, is synthesized matrix neurons. Melatonin reduces ETC elevates ATP production; it also detoxifies ROS/RNS via SIRT3/FOXO pathway upregulates activities superoxide dismutase 2 glutathione peroxidase. influences glucose processing by In neurogenerative diseases, often adopt Warburg-type metabolism which excludes pyruvate mitochondria causing reduced acetyl coenzyme A production. Acetyl supports citric acid cycle OXPHOS. Additionally, required co-substrate for arylalkylamine-N-acetyl transferase, rate limits melatonin synthesis; therefore, production diminished cells that experience making more vulnerable stress. Moreover, endogenously produced diminishes during aging, further increasing components. More normal preserved aging with supplementation.

Язык: Английский

Процитировано

14

Unveiling the Significance of Peroxiredoxin 6 in Central Nervous System Disorders DOI Creative Commons

Min Xue,

Xiaojie Huang,

Tong Zhu

и другие.

Antioxidants, Год журнала: 2024, Номер 13(4), С. 449 - 449

Опубликована: Апрель 10, 2024

Peroxiredoxin 6 (Prdx6), a unique 1-Cys member of the peroxiredoxin family, exhibits peroxidase activity, phospholipase and lysophosphatidylcholine acyltransferase (LPCAT) activity. Prdx6 has been known to be an important enzyme for maintenance lipid peroxidation repair, cellular metabolism, inflammatory signaling, antioxidant damage. Growing research demonstrated that altered activity this is linked with various pathological processes including central nervous system (CNS) disorders. This review discusses distinctive structure, function in different CNS disorders, as well emphasizing significance neurological

Язык: Английский

Процитировано

8

Melatonin Mitigates Acidosis-Induced Neuronal Damage by Up-Regulating Autophagy via the Transcription Factor EB DOI Open Access
Yan Shi,

Zhaoyu Mi,

Wei Zhao

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 1170 - 1170

Опубликована: Янв. 29, 2025

Acidosis, a common feature of cerebral ischemia and hypoxia, results in neuronal damage death. This study aimed to investigate the protective effects mechanisms action melatonin against acidosis-induced damage. SH-SY5Y cells were exposed an acidic environment simulate acidosis, photothrombotic (PT) infarction model was used establish animal male C57/BL6J mice. Both vivo vitro studies demonstrated that acidosis increased cytoplasmic transcription factor EB (TFEB) levels, reduced nuclear TFEB suppressed autophagy, as evidenced by elevated p62 higher LC3-II/LC3-I ratio, decreased synapse-associated proteins (PSD-95 synaptophysin), apoptosis. In contrast, promoted translocation TFEB, enhanced reversed Moreover, role melatonin’s neuroprotective validated modulating translocation. conclusion, mitigates promoting thereby enhancing autophagy. These findings offer new insights into potential treatments for acidosis.

Язык: Английский

Процитировано

0

Melatonin ameliorates ischemic brain injury in experimental stroke by regulation of miR-221 and ATG7 Axis DOI
Xu Liu, Yue Zhang, Yumei Ma

и другие.

Biochemical and Biophysical Research Communications, Год журнала: 2025, Номер unknown, С. 151706 - 151706

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

A ROS-responsive, aptamer-targeted graphene oxide nanocomposite for site-specific glutathione release in cerebral ischemia-reperfusion injury DOI Creative Commons
Meiying Li, Lili Wei, Ying Xu

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Май 14, 2025

Cerebral ischemia-reperfusion (I/R) injury is a major contributor to mortality and long-term disability worldwide, primarily due excessive reactive oxygen species (ROS) generation after blood flow restored. Although current treatments focus on reestablishing perfusion, they offer limited protection against the secondary ROS-mediated injury. Here, we report multifunctional nanocomposite-graphene oxide loaded with glutathione (GSH) functionalized fibrinogen-targeting aptamer (GO@GSH-FA)-capable of selectively releasing antioxidant cargo within ischemic brain microenvironment. Characterization revealed drug-loading capacity 17.59% ± 3.74% an entrapment efficiency 78.78% 4.55%, highlighting robust loading GSH. The ROS-sensitive borate ester linker ensures that GSH preferentially liberated in oxidative stress regions, while fibrinogen actively targets fibrin-rich thrombotic sites. In vitro, GO@GSH-FA significantly restored viability oxygen-glucose-deprived SH-SY5Y cells (from 31% up near control levels), reduced inflammatory cytokines, lowered intracellular ROS. Endothelin-1 (ET-1) induced cortical ischemia model, led marked decrease neurological deficit scores 7.20 1.16 4.20 0.98) enhanced neuronal survival relative untreated animals. Collectively, these findings underscore promise as targeted, ROS-responsive platform for mitigating cerebral I/R

Язык: Английский

Процитировано

0

Factors Contributing to Resistance to Ischemia-Reperfusion Injury in Olfactory Mitral Cells DOI Open Access
Choong‐Hyun Lee, Ji Hyeon Ahn, Moo‐Ho Won

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(11), С. 5079 - 5079

Опубликована: Май 25, 2025

Brain ischemia-reperfusion (IR) injury is a critical pathological process that leads to extensive neuronal death, with hippocampal pyramidal cells, particularly those in the cornu Ammonis 1 (CA1) subfield, being highly vulnerable. Until now, human olfactory mitral cell resistance IR has not been directly studied, but dysfunction humans frequently reported systemic vascular conditions such as ischemic heart failure and may serve an early clinical marker of neurological or cardiovascular disease. Mitral principal neurons bulb (OB), exhibit remarkable injury, suggesting presence unique molecular adaptations support their survival under stress. Several factors contribute resilience cells. They have lower susceptibility excitotoxicity, mitigating harmful effects excessive glutamate signaling. Additionally, they maintain efficient calcium homeostasis, preventing overload—a major trigger for death vulnerable neurons. cells also express high baseline levels antioxidant enzymes activities, counteracting oxidative Their robust mitochondrial function enhances energy production reduces metabolic failure. Furthermore, neuroprotective signaling pathways, including phosphatidylinositol-3-kinase (PI3K)/Akt, mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK), nuclear factor erythroid-2-related 2 (Nrf2)-mediated antioxidative responses, further bolster resistance. In addition these intrinsic mechanisms, microvascular architecture within provide extra layer protection. By comparing ischemia-sensitive neurons, key vulnerabilities—such stress, dysregulation, dysfunction—can be identified potentially mitigated other brain regions. Understanding determinants offer valuable insights developing novel strategies combat areas, hippocampus cortex.

Язык: Английский

Процитировано

0

Skimmianine Showed Neuroprotection against Cerebral Ischemia/Reperfusion Injury DOI Creative Commons
Hayat Ayaz, Fırat Aşır, Tuğcan Korak

и другие.

Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(7), С. 7373 - 7385

Опубликована: Июль 12, 2024

The aim of this study was to investigate the antioxidant and anti-inflammatory effects skimmianine on cerebral ischemia–reperfusion (IR) injury. Twenty-four female Wistar albino rats were randomly divided into three groups: Sham, Ischemia–Reperfusion (IR), IR + Skimmianine (40 mg/kg Skimmianine). Cerebral ischemia induced using a monofilament nylon suture occlude middle artery for 60 min. Following 23 h reperfusion, animals sacrificed 14 days later. brain tissue post-IR injury examined through biochemical immunochemical analyses. In silico analysis Enrichr platform explored skimmianine’s potential biological processes involving IBA-1, IL-6, NF-κB proteins. group, MDA levels increased, while SOD CAT enzyme activities decreased. treatment resulted in decreased increased activities. Significant increases IBA-1 expression observed which significantly reduced, modulating microglial activation. High IL-6 noted pyramidal neurons, vascular structures, neuroglial cells group; reduced expression, demonstrating effects. Increased neurons blood vessels gray white matter expression. Gene Ontology results suggest impacts immune inflammatory responses via with estrogen mechanisms mediated by NF-κB. may be therapeutic strategy due its

Язык: Английский

Процитировано

2

Protective effect of cinnamon extract against cobalt-induced multiple organ damage in rats DOI Creative Commons
B. Isik, Bahadır Süleyman, Renad Mammadov

и другие.

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Май 9, 2024

Background The role of oxidative stress and inflammation in cobalt (Co) toxicity has been the focus previous studies. Cinnamon its main components have reported to protective effects various tissues with antioxidant anti-inflammatory effects. Aims In this study, effect cinnamon extract (CE) against possible Co-induced heart, kidney, liver damage rats was investigated biochemically. Methods Eighteen albino Wistar -type male were categorized into three groups ( n = 6 per group): control (CG), CoCL 2 -administered (CoCL ), CE + (CE Co) groups. group administered (100 mg/kg), CG distilled water orally by gavage. One hour after administration, Co (150 mg/kg) This procedure repeated once daily for 7 days. Then, biochemical markers studied excised tissues. Results increased oxidants proinflammatory cytokines decreased antioxidants Heart, tissue affected damage. treatment suppressed -induced increase decrease shown attenuate cardiac reducing serum troponin I (TpI) creatine kinase-MB (CK-MB), renal creatinine blood urea nitrogen (BUN), alanine aminotransferase (ALT) aspartate (AST). Conclusion induced production parameters depletion rats. Our experimental results show that protects inflammatory changes CoCLl .

Язык: Английский

Процитировано

0

Neuroprotective effects of Hibiscus sabdariffa var. altissima on cerebral ischemia‒Reperfusion injury in rats DOI Creative Commons
Adjia Hamadjida,

Saida Nkuketgnigni Njemguie,

Rigobert Espoir Ayissi Mbomo

и другие.

Pharmacological Research - Modern Chinese Medicine, Год журнала: 2024, Номер 12, С. 100485 - 100485

Опубликована: Авг. 2, 2024

Hibiscus sabdariffa var. altissima, which is known for its high-quality fiber, commonly used in Traditional Chinese Medicine (TCM) diuretic, choleretic, analgesic, antitussive, and hypotensive effects. Furthermore, the flowers have been to treat diabetes, hypertension, atherosclerosis, obesity. However, there are no studies assessing neuroprotective effects on cerebral ischemia, unlike variety Therefore, this study was aimed investigate potential efficacy of altissima extract (HAS) underlying mechanism oxidative stress neuroinflammation during ischemia-reperfusion (I/R) injury. A model ischemia established male Wistar rats through middle artery occlusion (MCAO) reperfusion. Rats were randomly divided into sham, IR, IR + HSA100, HSA200, HSA400 Eda groups treated 14 consecutive days. The neurological deficit score cylinder test performed assess impairment. Oxidative determined by measuring levels malondialdehyde (MDA) antioxidant markers such as reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) measured evaluate activities. In addition, inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), also determined. Our results demonstrated that HSA significantly ameliorated impairment volume brain infarct. decreased MDA enhanced activities GSH, SOD CAT tissues. expression proinflammatory cytokines TNF-α, IL-1β IL-6 serum. These findings exhibited a effect against I/R injury, possibly improving attenuating responses. could be therapeutic option ischemic injuries.

Язык: Английский

Процитировано

0