AlphaFold-Based AI Docking Reveals AMPK/SIRT1-TFEB Pathway Modulation by Traditional Chinese Medicine in Metabolic-Associated Fatty Liver Disease
Pharmacological Research,
Год журнала:
2025,
Номер
unknown, С. 107617 - 107617
Опубликована: Янв. 1, 2025
Язык: Английский
The efficacy of traditional Chinese medicine sequential therapy in non-alcoholic fatty liver disease
Pakistan Journal of Medical Sciences,
Год журнала:
2025,
Номер
41(2), С. 512 - 518
Опубликована: Янв. 23, 2025
Objective:
Sequential
therapy
in
traditional
Chinese
medicine
(TCM)
refers
to
a
combination
of
internal
and
external
treatment
methods
certain
order
based
on
syndrome
differentiation
therapy.
The
aim
this
study
was
evaluate
the
efficacy
TCM
sequential
that
is
given
basis
conventional
management
patients
with
non-alcoholic
fatty
liver
disease
(NAFLD).
Methods:
Medical
records
one
hundred
NAFLD
who
received
at
Zhejiang
Provincial
Tongde
Hospital
between
February
2023
April
2024
were
retrospectively
analyzed.
Of
them,
48
routine
intervention
(Control
group),
52
additionally
treated
by
(TCM
group).
levels
blood
lipid
indicators,
function
efficacy,
quality
life
(QOL)
scores
compared
two
groups
before
after
intervention.
Results:
After
intervention,
indicators
both
significantly
improved
preintervention
considerably
better
Control
group
(P<0.05).
overall
higher
(94.23%)
(79.17%)
QOL
increased
than
Conclusions:
For
patients,
adopting
addition
strategies
can
effectively
regulate
patients’
levels,
restore
function,
improve
effectiveness,
life.
doi:
https://doi.org/10.12669/pjms.41.2.11352
How
cite
this:
Zhang
L,
Qian
Y,
Wu
H,
Xu
H.
disease.
Pak
J
Med
Sci.
2025;41(2):512-518.
This
an
Open
Access
article
distributed
under
terms
Creative
Commons
Attribution
License
(http://creativecommons.org/licenses/by/3.0),
which
permits
unrestricted
use,
distribution,
reproduction
any
medium,
provided
original
work
properly
cited.
Язык: Английский
Patent research in academic literature. Landscape and trends with a focus on patent analytics
Frontiers in Research Metrics and Analytics,
Год журнала:
2025,
Номер
9
Опубликована: Янв. 8, 2025
Patent
analytics
is
crucial
for
understanding
innovation
dynamics
and
technological
trends.
However,
a
comprehensive
overview
of
this
rapidly
evolving
field
lacking.
This
study
presents
data-driven
analysis
patent
research,
employing
citation
network
to
categorize
examine
research
clusters.
Here,
we
show
that
characterized
by
interconnected
themes
spanning
fundamental
systems,
indicator
development,
methodological
advancements,
intellectual
property
management
practices,
diverse
applications.
We
reveal
central
areas
in
strategies,
impact,
while
identifying
emerging
focuses
on
environmental
sustainability
corporate
innovation.
The
integration
advanced
analytical
techniques,
including
AI
machine
learning,
observed
across
various
domains.
provides
insights
researchers
practitioners,
highlighting
opportunities
cross-disciplinary
collaboration
future
directions.
Язык: Английский
Exploring the mechanism of SLXG for treating nonalcoholic fatty liver disease based on network pharmacology and molecular docking
Medicine,
Год журнала:
2025,
Номер
104(6), С. e40255 - e40255
Опубликована: Фев. 7, 2025
Background:
The
Shugan
Lidan
Decoction
and
Chaihu
formula
are
traditional
Chinese
medicine
formulas
for
treating
liver
diseases,
with
a
history
of
over
1000
years.
By
comprehensively
improving
2
medicinal
formulas,
Xiaoshi
Granules
(SLXG)
has
been
developed
the
treatment
nonalcoholic
fatty
disease
(NAFLD)
other
liver-related
metabolic
diseases.
Methods:
First,
effective
active
ingredients
targets
SLXG
were
determined
using
Traditional
Medicine
Systems
Pharmacology
Database
Analysis
Platform
database.
NAFLD
identified
GeneCards,
OMIM,
CTD
databases,
intersection
decoction
was
obtained.
then
subjected
to
protein–protein
interaction
network
analysis,
Kyoto
encyclopedia
genes
genomes
(KEGG)
enrichment
gene
ontology
analysis.
KEGG
analysis
revealed
pathway.
Molecular
docking
performed
validate
binding
between
crucial
enriched
in
this
pathway
corresponding
SLXG.
Results:
A
total
219
related
from
239
non-duplicated
drug
obtained
24
target
both
drug-
disease-related
6
results
showed
that
13
gene–active
ingredient
bindings
had
energy
less
than
−6.5.
Conclusion:
use
pharmacology
molecular
technology
mechanism
action
treatment,
thus
laying
theoretical
foundation
clinical
application
therapy.
Язык: Английский
Analysis the Mechanism of <i>Herba</i> <i>Artemisiae</i> <i>s</i><i>copariae</i> and <i>Folium </i><i>n</i><i>elumbinis</i> in the Treatment of Non-Alcoholic Fatty Liver Disease Based on Network Pharmacology
Traditional Chinese Medicine,
Год журнала:
2025,
Номер
14(04), С. 1253 - 1262
Опубликована: Янв. 1, 2025
Deciphering the mechanism of Chaihu Shugan San in the treatment of nonalcoholic steatohepatitis using network pharmacology and molecular docking
Journal of Pharmacy and Pharmacology,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 9, 2024
Abstract
Objectives
In
China,
there
is
a
long
history
and
rich
clinical
experience
in
treating
nonalcoholic
steatohepatitis
(NASH)
with
traditional
Chinese
herbal
medicines,
including
Chai
Hu
Shu
Gan
San.
This
study
aims
to
investigate
the
potential
regulatory
effects
of
Chaihu
Shugan
San
(CSS)
on
liver
lipid
metabolism
inflammatory
damage
mice
experimental
induced
by
choline-deficient
high-fat
diet
(CDHFD).
Utilizing
network
pharmacology,
we
systematically
explore
mechanisms
action
therapeutic
CSS
against
NASH.
Methods
Potential
targets
for
NASH
were
identified
using
online
databases.
Functional
enrichment
protein–protein
interaction
analyses
conducted
identify
hub-targeted
genes
elucidate
underlying
molecular
mechanisms.
The
affinities
active
compounds
evaluated
docking.
Finally,
validated
through
real-time
polymerase
chain
reaction,
western
blotting,
immunofluorescence
mice,
both
without
treatment.
Key
findings
reduces
serum
ALT
AST
levels
mice(P
<
0.05)
ameliorates
ballooning
degeneration
livers
thereby
lowering
NAS
score(P
0.05).
Including
naringenin,
high-performance
liquid
chromatography/mass
spectrometrys
12
chromatographic
peaks.
Based
pharmacology
analysis,
contains
total
103
877
target
genes.
Transferase
activity
represents
mechanism
intervention
transcriptional
protein
expression
SIRT1
gene
are
significantly
increased
(P
Conclusions
Naringenin
probable
compound
hub
which
involved
Язык: Английский
Elucidation of the anti-fibrotic diseases mechanism of chelerythrine by integrative approach of network pharmacology and experimental verification
Natural Product Research,
Год журнала:
2024,
Номер
unknown, С. 1 - 7
Опубликована: Авг. 18, 2024
In
the
present
study,
we
conducted
an
integrative
approach
of
network
pharmacology
and
experimental
validation
study
to
elucidate
underlying
mechanisms
Chelerythrine
(CLT),
in
treating
fibrotic
diseases
(FD),
which
are
disorders
characterised
by
excessive
accumulation
extracellular
matrix.
27
common
targets
CLT
against
FD
were
analysed,
these
used
construct
PPI
network.
The
results
GO
KEGG
enrichment
analyses
suggested
that
exerted
pharmacological
effects
on
regulating
mTOR
signalling
pathway,
AKT-PI3K
pathway
apoptosis
pathway.
Finally,
molecular
docking
confirmed
a
strong
binding
affinity
between
core
target
proteins.
has
inhibitory
proliferation
migration
L929
cells,
could
promote
cell
apoptosis.
decreased
levels
Bcl-2,
p-AKT/AKT,
p-mTOR/mTOR
p-PI3K/PI3K,
meanwhile
increased
Bax.
Taken
together,
indicate
may
be
potential
drug
for
anti-fibrotic
therapy.
Язык: Английский
Bioinformatics based exploration of the anti-NAFLD mechanism of Wang’s empirical formula via TLR4/NF-κB/COX2 pathway
Molecular Medicine,
Год журнала:
2024,
Номер
30(1)
Опубликована: Дек. 27, 2024
Abstract
Background
Nonalcoholic
fatty
liver
disease
(NAFLD)
has
developed
as
a
leading
public
wellness
challenge
result
of
changes
in
dietary
patterns.
Unfortunately,
there
is
still
lack
effective
pharmacotherapy
methods
for
NAFLD.
Wang’s
empirical
formula
(WSF)
demonstrated
considerable
clinical
efficacy
treating
metabolic
disorders
years.
Nevertheless,
the
protective
effect
WSF
against
NAFLD
and
its
underlying
mechanism
remains
poorly
understood.
Methods
The
model
was
established
using
17-week
high-sucrose
high-fat
(HSHF)
diet
with
32
ICR
mice.
In
assessing
therapeutic
on
NAFLD,
we
detected
body
weight,
viscera
biomarkers
glycolipid
metabolism
serum
liver,
transaminase
levels
histopathology
H&E
Oil
Red
O
staining
after
oral
administration.
chemical
components
were
extensively
identified
gathered
utilizing
HPLC-Q-TOF/MS
system,
database
mining
from
HMDB,
MassBank,
TCMSP
databases,
alongside
literature
searches
CNKI,
Wanfang
VIP
databases.
forecast
network
pharmacology
approach
then
utilized
to
investigate
probable
mechanisms
by
which
improves
based
performance
prospective
target
identification
pathway
enrichment
analysis.
Besides,
molecular
docking
also
conducted
verification
combination
activities
between
active
core
proteins
related
final,
validation
experiments
obtained
pathways
through
ELISA,
immunohistochemistry
(IHC),
western
blot
(WB)
Results
Pharmacodynamic
outcomes
indicated
that
intervention
effectively
mitigated
obesity,
fat
accumulation
organs,
lipid
disorders,
abnormal
pathology
injury
mice
(
P
<
0.05,
0.01).
A
total
72
existent
ingredients
acquired
database,
254
common
targets
(11.6%
targets)
identified.
Network
revealed
presses
via
modulating
TNF,
IL6,
AKT1,
IL1B,
PTGS2
(COX2),
other
targets,
primarily
inflammatory
signaling
pathways,
confirmed
docking.
Molecular
biology
further
conformed
could
decrease
factors
like
IL-1β,
IL-6
TNF-α
0.01)
expression
TLR4,
NF-κB
COX-2
liver.
Conclusion
treatment
protects
inflammation
HSHF
diet-induced
mice,
might
be
suppressing
TLR4/NF-κB/COX-2
reduce
release
cytokines
Graphical
abstract
Язык: Английский