Antioxidants,
Год журнала:
2024,
Номер
13(6), С. 651 - 651
Опубликована: Май 27, 2024
Alzheimer’s
disease
(AD)
is
a
stealthy
and
progressive
neurological
disorder
that
leading
cause
of
dementia
in
the
global
elderly
population,
imposing
significant
burden
on
both
society.
Currently,
condition
treated
with
medications
alleviate
symptoms.
Nonetheless,
these
drugs
may
not
consistently
produce
desired
results
can
serious
side
effects.
Hence,
there
vigorous
pursuit
alternative
options
to
enhance
quality
life
for
patients.
Ginkgo
biloba
(GB),
an
herb
historical
use
traditional
medicine,
contains
bioactive
compounds
such
as
terpenoids
(Ginkgolides
A,
B,
C),
polyphenols,
organic
acids,
flavonoids
(quercetin,
kaempferol,
isorhamnetin).
These
are
associated
anti-inflammatory,
antioxidant,
neuroprotective
properties,
making
them
valuable
cognitive
health.
A
systematic
search
across
three
databases
using
specific
keywords—GB
AD
dementia—yielded
1702
documents,
selection
15
clinical
trials
synthesis.
In
eleven
studies,
GB
extract/EGb
761®
was
shown
improve
function,
neuropsychiatric
symptoms,
functional
abilities
types.
four
however,
were
no
differences
between
GB-treated
placebo
groups.
Significant
improvements
observed
scores
obtained
from
Mini-Mental
State
Examination
(MMSE),
Short
Cognitive
Performance
Test
(SKT),
Neuropsychiatric
Inventory
(NPI).
While
majority
synthesized
show
has
promising
potential
treatment
conditions,
more
research
needed
determine
optimal
dosages,
effective
delivery
methods,
appropriate
pharmaceutical
formulations.
Furthermore,
thorough
assessment
adverse
effects,
exploration
long-term
implications,
investigation
into
drug
interactions
critical
aspects
must
be
carefully
evaluated
future
studies.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(5), С. 2512 - 2512
Опубликована: Фев. 21, 2024
Bone
differentiation
is
crucial
for
skeletal
development
and
maintenance.
Its
dysfunction
can
cause
various
pathological
conditions
such
as
rickets,
osteoporosis,
osteogenesis
imperfecta,
or
Paget's
disease.
Although
traditional
two-dimensional
cell
culture
systems
have
contributed
significantly
to
our
understanding
of
bone
biology,
they
fail
replicate
the
intricate
biotic
environment
tissue.
Three-dimensional
(3D)
spheroid
cultures
gained
widespread
popularity
addressing
defects.
This
review
highlights
advantages
employing
3D
investigate
differentiation.
It
their
capacity
mimic
complex
in
vivo
cellular
interactions
pivotal
homeostasis.
The
exploration
models
research
offers
enhanced
physiological
relevance,
improved
predictive
capabilities,
reduced
reliance
on
animal
models,
which
advancement
safer
more
effective
strategies
drug
development.
Studies
highlighted
transformative
potential
expanding
biology
developing
targeted
therapeutic
interventions
bone-related
disorders.
explores
how
demonstrated
promise
unraveling
mechanisms
governing
homeostasis
responses
pharmacological
agents.
Stem Cell Research & Therapy,
Год журнала:
2024,
Номер
15(1)
Опубликована: Янв. 17, 2024
Abstract
Background
After
myocardial
infarction,
the
lost
myocardium
is
replaced
by
fibrotic
tissue,
eventually
progressively
leading
to
dysfunction.
Direct
reprogramming
of
fibroblasts
into
cardiomyocytes
via
forced
overexpression
cardiac
transcription
factors
Gata4,
Mef2c,
and
Tbx5
(GMT)
offers
a
promising
strategy
for
repair.
The
limited
efficiency
this
approach,
however,
remains
significant
challenge.
Methods
We
screened
seven
capable
improving
direct
both
mice
human
evaluating
small
molecules
known
be
involved
in
cardiomyocyte
differentiation
or
promoting
human-induced
pluripotent
stem
cell
reprogramming.
Results
found
that
vitamin
C
(VitC)
significantly
increased
when
added
GMT-overexpressing
from
2D
3D
model.
observed
increase
reactive
oxygen
species
(ROS)
generation
upon
Doxy
induction,
ROS
was
subsequently
reduced
VitC
treatment,
associated
with
efficiency.
However,
treatment
dehydroascorbic
acid,
structural
analog
but
lacking
antioxidant
properties,
no
difference
observed,
suggesting
effect
enhancing
partly
dependent
its
properties.
Conclusions
Our
findings
demonstrate
supplementation
enhances
reprogramming,
partially
suppressing
production
presence
GMT.
Graphical
abstract
Cells,
Год журнала:
2024,
Номер
13(12), С. 1054 - 1054
Опубликована: Июнь 18, 2024
Over
the
past
decade,
development
of
three-dimensional
(3D)
models
has
increased
exponentially,
facilitating
unravelling
fundamental
and
essential
cellular
mechanisms
by
which
cells
communicate
with
each
other,
assemble
into
tissues
organs
respond
to
biochemical
biophysical
stimuli
under
both
physiological
pathological
conditions.
This
section
presents
a
concise
overview
most
recent
updates
on
significant
contribution
different
types
3D
cell
cultures
including
spheroids,
organoids
organ-on-chip
bio-printed
in
advancing
our
understanding
molecular
mechanisms.
The
case
studies
presented
include
breast
cancer
(BC),
endometriosis,
liver
microenvironment
infections.
In
BC,
establishment
culture
permitted
visualization
role
cancer-associated
fibroblasts
delivery
exosomes,
as
well
significance
physical
properties
extracellular
matrix
promoting
proliferation
invasion.
approach
also
become
valuable
tool
gaining
insight
general
specific
drug
resistance.
Given
considerable
heterogeneity
offer
more
accurate
representation
vivo
microenvironment,
thereby
identification
translation
novel
targeted
therapeutic
strategies.
advantages
provided
hepatic
environment,
conjunction
high
throughput
characterizing
various
platforms,
have
enabled
elucidation
complex
underlying
threatening
diseases.
A
limited
number
for
gut
skin
infections
been
developed.
However,
profound
comprehension
spatial
temporal
interactions
between
microbes,
host
their
environment
may
facilitate
advancement
vitro,
ex
disease
models.
Additionally,
it
pave
way
approaches
diverse
research
fields.
interested
reader
will
find
concluding
remarks
challenges
prospects
using
discovering
areas
covered
this
review.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 15, 2025
Nuclear
magnetic
resonance
(NMR)
spectroscopy
is
a
valuable
diagnostic
tool
limited
by
low
sensitivity
due
to
nuclear
spin
polarization.
Hyperpolarization
techniques,
such
as
dissolution
dynamic
polarization,
significantly
enhance
sensitivity,
enabling
real-time
tracking
of
cellular
metabolism.
However,
traditional
high-field
NMR
systems
and
bioreactor
platforms
pose
challenges,
including
the
need
for
specialized
equipment
fixed
sample
volumes.
This
study
introduces
scalable,
3D-printed
platform
compatible
with
low-field
spectrometers,
designed
accommodate
bioengineered
3D
cell
models.
The
fabricated
using
biocompatible
materials
features
microfluidic
system
media
recirculation,
ensuring
optimal
culture
conditions
during
acquisition
maintenance.
We
characterized
compatibility
components
confirmed
minimal
signal
distortion.
bioreactor's
efficacy
was
validated
HeLa
HepG2
cells,
demonstrating
prolonged
viability
enhanced
metabolic
activity
in
cultures
compared
2D
cultures.
Hyperpolarized
[1-13C]
pyruvate
experiments
revealed
distinct
profiles
two
types,
highlighting
ability
discern
among
samples.
Our
results
indicate
that
supports
maintenance
analysis
models
studies,
offering
versatile
accessible
biochemical
research
tissue
engineering.
bridges
gap
between
advanced
spectroscopy,
providing
robust
framework
future
applications
nonspecialized
laboratories.
design
files
printed
are
shared
within
text
easy
download
customization,
promoting
their
use
adaptation
further
applications.
ACS Applied Materials & Interfaces,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 29, 2025
Traditional
cell
culture
methods
face
significant
limitations
in
monitoring
secretions
with
spatial
and
temporal
precision.
Advanced
microsystems
incorporating
biosensors
have
been
developed
to
address
these
challenges,
but
they
tend
lack
versatility,
their
complexity,
along
the
requirement
for
specialized
equipment,
limits
broader
adoption.
CellStudio
offers
an
innovative,
user-friendly
solution
that
exploits
Printing
Vacuum
Lithography
combined
bead-based
assays
create
modular
tunable
patterns
surrounded
by
biosensors.
This
platform
allows
high-resolution,
spatially
resolved
analysis
of
secreted
proteins,
such
as
VEGF
FGF-2,
while
being
easily
implementable
standard
laboratory
settings.
CellStudio's
design
is
compatible
conventional
facilitating
its
integration
into
existing
workflows
without
need
extensive
training
or
tools.
Validation
experiments
using
mesenchymal
stem
cells
HeLa
demonstrated
can
detect
small
secretion
levels
from
clusters
high
sensitivity
analyze
diffusion
profiles,
remarking
possibilities
studying
behavior.
By
offering
a
standardized,
cost-effective
approach
detailed
cellular
analysis,
significantly
enhances
capabilities
traditional
techniques
broad
applications
across
biological
biomedical
research.
Cancers,
Год журнала:
2025,
Номер
17(4), С. 700 - 700
Опубликована: Фев. 19, 2025
The
development
of
anticancer
therapies
has
increasingly
relied
on
advanced
3D
in
vitro
models,
which
more
accurately
mimic
the
tumor
microenvironment
compared
to
traditional
2D
cultures.
This
review
describes
evolution
these
highlighting
significant
advancements
and
their
impact
cancer
research.
We
discuss
integration
machine
learning
(ML)
artificial
intelligence
(AI)
enhancing
predictive
power
efficiency
potentially
reducing
dependence
animal
testing.
ML
AI
offer
innovative
approaches
for
analyzing
complex
data,
optimizing
experimental
conditions,
predicting
therapeutic
outcomes
with
higher
accuracy.
By
leveraging
technologies,
next
generation
models
could
revolutionize
drug
development,
offering
effective
alternatives
experiments.
