Fish & Shellfish Immunology, Год журнала: 2023, Номер 143, С. 109185 - 109185
Опубликована: Окт. 30, 2023
Язык: Английский
Опубликована: Май 9, 2025
Abstract In many organisms, including Drosophila and humans, neural progenitors exit the cell cycle are eliminated by end of development, which retricts adult neurogenesis to specific brain regions such as mammalian hippocampus. Here, we show that conserved transcription factor Krüppel (Kr) regulates proliferation neurogenic capacity mushroom body neuroblasts (MBNBs), generate learning memory center in brain, functionally analogous Neuroblast-specific Kr RNAi Irregular facet (KrIf-1) mutation extends MBNB lifespan, enabling continued brain. is expressed at low levels postembryonic MBNBs, its pupal stage-specific depletion sufficient induce retention, distinguishing this role from established function embryonic neurogenesis. Persisting MBNBs maintain expression RNA-binding protein IGF-2-binding (Imp), promotes early neuronal fate. Co-depletion Imp abolishes extended induced depletion. Additionally, homolog 1 (Kr-h1), another family a key regulator hormone-mediated transcription, antagonises Kr’s function: knockdown suppresses phenotype while overexpression drives tumour-like neuroblast overgrowths. These findings define lineage-specific regulatory axis governing Drosophila, with potential parallels other organisms.
Язык: Английский
Процитировано
0
Опубликована: Май 9, 2025
Abstract In many organisms, including Drosophila and humans, neural progenitors exit the cell cycle are eliminated by end of development, which retricts adult neurogenesis to specific brain regions such as mammalian hippocampus. Here, we show that conserved transcription factor Krüppel (Kr) regulates proliferation neurogenic capacity mushroom body neuroblasts (MBNBs), generate learning memory center in brain, functionally analogous Neuroblast-specific Kr RNAi Irregular facet (KrIf-1) mutation extends MBNB lifespan, enabling continued brain. is expressed at low levels postembryonic MBNBs, its pupal stage-specific depletion sufficient induce retention, distinguishing this role from established function embryonic neurogenesis. Persisting MBNBs maintain expression RNA-binding protein IGF-2-binding (Imp), promotes early neuronal fate. Co-depletion Imp abolishes extended induced depletion. Additionally, homolog 1 (Kr-h1), another family a key regulator hormone-mediated transcription, antagonises Kr’s function: knockdown suppresses phenotype while overexpression drives tumour-like neuroblast overgrowths. These findings define lineage-specific regulatory axis governing Drosophila, with potential parallels other organisms.
Язык: Английский
Процитировано
0
Elsevier eBooks, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Процитировано
0
eLife, Год журнала: 2023, Номер 12
Опубликована: Ноя. 21, 2023
To gain insights into how juvenile hormone (JH) came to regulate insect metamorphosis, we studied its function in the ametabolous firebrat, Thermobia domestica. Highest levels of JH occur during late embryogenesis, with only low thereafter. Loss-of-function and gain-of-function experiments show that acts on embryonic tissues suppress morphogenesis cell determination promote their terminal differentiation. Similar actions hemimetabolous insects short germ band embryos indicate JH's role preceded derived as postembryonic regulator metamorphosis. The expansion likely followed evolution flight. Archaic flying were considered lack metamorphosis because tiny, movable wings evident thoraces young juveniles positive allometric growth eventually allowed them support flight juveniles. Like Thermobia, assume these lacked JH. However, a reappearance wing redirected development make pad rather than wing. Maintenance then disappearance mature Subsequent modification action for hemi- holometabolous lifestyles are discussed.
Язык: Английский
Процитировано
8
Опубликована: Янв. 13, 2024
Exoskeletons are a defining character of all arthropods that provide physical support for their segmented bodies and appendages as well protection from the environment predation. This ubiquitous yet evolutionarily variable feature has been instrumental in facilitating adoption variety lifestyles exploitation ecological niches across environments. Throughout radiation Arthropoda produced more than one million described modern species, adaptability afforded by segmentation exoskeletons led to diversity is unrivalled amongst animals. However, because cannot grow, they must be periodically shed replaced with new larger ones accommodate growing individuals encased within. Arthropods therefore undergo periodic moulting events, which follow series steps preparatory pre-moult phase ecdysis itself post-moult maturation exoskeleton. Each event represents particularly vulnerable period an arthropod’s life cycle, so process tightly regulated meticulously executed ensure successful transitions normal growth development. Decades research representative foundation understanding mechanisms involved. Building on this, studies continue develop test hypotheses presence function molecular components, including neuropeptides, hormones, receptors, so-called early, late, fate genes, arthropod diversity. Here, we review literature comprehensive overview current status accumulated knowledge genetic toolkit governing moulting. From biosynthesis regulation ecdysteroid sesquiterpenoid factors involved hormonal stimulation responses exoskeleton remodelling, identify commonalities differences, highlighting major gaps, groups. We examine available evidence supporting models how components operate together prepare for, execute, recover ecdysis, comparing reports Chelicerata, Myriapoda, Crustacea, Hexapoda. Evidence generally highly taxonomically imbalanced, most based insect study systems. Biases also evident different phases processes, early triggers late effectors being least explored. Our synthesis contrasts reported observations reasonably plausible assumptions given taxonomic sampling, exposes weak or gaps need addressing. Encouragingly, advances genomics driving diversification tractable systems cataloguing putative toolkits previously under-explored taxa. Analysis genome transcriptome data supported experimental investigations have validated “ultra-conserved” core genes. The machinery likely evolved elaborations this conserved pathway backbone, but exploration needed characterise lineage-specific changes novelties. Furthermore, linking these transformative innovations processes remains hampered untested assumptions. Promisingly however, emerging framework highlights avenues underlying genetics dynamic biology through complex physiology
Язык: Английский
Процитировано
2
eLife, Год журнала: 2024, Номер 12
Опубликована: Апрель 3, 2024
To gain insights into how juvenile hormone (JH) came to regulate insect metamorphosis, we studied its function in the ametabolous firebrat, Thermobia domestica . Highest levels of JH occur during late embryogenesis, with only low thereafter. Loss-of-function and gain-of-function experiments show that acts on embryonic tissues suppress morphogenesis cell determination promote their terminal differentiation. Similar actions hemimetabolous insects short germ band embryos indicate JH’s role preceded derived as postembryonic regulator metamorphosis. The expansion likely followed evolution flight. Archaic flying were considered lack metamorphosis because tiny, movable wings evident thoraces young juveniles positive allometric growth eventually allowed them support flight juveniles. Like , assume these lacked JH. However, a reappearance wing redirected development make pad rather than wing. Maintenance then disappearance mature Subsequent modification action for hemi- holometabolous lifestyles are discussed.
Язык: Английский
Процитировано
2
International Journal of Biological Macromolecules, Год журнала: 2024, Номер 276, С. 133402 - 133402
Опубликована: Сен. 1, 2024
Язык: Английский
Процитировано
2
PLoS Biology, Год журнала: 2024, Номер 22(8), С. e3002763 - e3002763
Опубликована: Авг. 12, 2024
Phenotypic plasticity displayed by an animal in response to different environmental conditions is supposedly crucial for its survival and reproduction. The female adults of some ant lineages display phenotypic related reproductive role. In pharaoh queens, insemination induces substantial physiological/behavioral changes implicates remarkable gene regulatory network (GRN) shift the brain. Here, we report a neuropeptide neuroparsin A ( NPA ) showing conserved expression pattern associated with activity across species. Knock-down unmated queen enhances ovary activity, whereas injection peptide fertilized suppresses activity. We found that mainly affected downstream JHBP ovary, which positively regulated suppression elevated shadow negatively NPA. Furthermore, show was also employed into brain–ovary axis regulating worker other distantly species, such as Harpegnathos venator ants.
Язык: Английский
Процитировано
2
Current Opinion in Insect Science, Год журнала: 2024, Номер 66, С. 101256 - 101256
Опубликована: Авг. 28, 2024
Язык: Английский
Процитировано
2
Current Opinion in Insect Science, Год журнала: 2024, Номер 67, С. 101289 - 101289
Опубликована: Окт. 30, 2024
Язык: Английский
Процитировано
2