Fish & Shellfish Immunology, Год журнала: 2023, Номер 143, С. 109185 - 109185
Опубликована: Окт. 30, 2023
Язык: Английский
Frontiers in Physiology, Год журнала: 2023, Номер 14
Опубликована: Март 15, 2023
The nuclear receptors HR3 and FTZ-F1 are highly conserved function to regulate molting reproduction in both hemimetabolous holometabolous insects. However, their roles Nilaparvata lugens largely unknown. In the present study, we discover that NlHR3 NlFTZ-F1 activated nymph stages by ecdysone signaling. Transcription disruption of expression prevents ecdysis metamorphosis, which leads abnormal appearance, malformed ovaries, lethal phenotypes. addition, demonstrate interacting with intrinsic 20E JH signaling pathways. Our work offers a deep insight into action mechanisms Moreover, could properly be exploited as potential target genes for developing RNAi-based pesticides control N. lugens.
Язык: Английский
Процитировано
5
Insect Biochemistry and Molecular Biology, Год журнала: 2023, Номер 157, С. 103957 - 103957
Опубликована: Май 15, 2023
Язык: Английский
Процитировано
4
Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(2), С. 1335 - 1347
Опубликована: Фев. 3, 2024
Bumblebees (B. terrestris) play a crucial role as highly efficient biological agents in commercial pollination. Understanding the molecular mechanisms governing their adaptation to diverse seasonal environments may pave way for effective management strategies future. With burgeoning advancement post-genetic studies focusing on B. terrestris, there is critical need normalize quantitative real-time PCR (qRT-PCR) data using suitable reference genes. To address this necessity, we employed RefFinder, software-based tool, assess suitability of several candidate endogenous control genes, including actin (ACT), arginine kinase (AK), elongation factor 1 alpha (EF1), glyceraldehyde-3-phosphate (GAPDH), phospholipase (PLA2), and ribosomal proteins (S18, S28). These genes were evaluated efficacy controls by examining expression patterns across various environmental conditions corresponding different seasons (Spring, Summer, Autumn, Winter) tissues (ovary, fat body, thorax, head) bumblebees. Moreover, study investigated significance selecting appropriate three key involved juvenile hormone (JH) signaling pathways: Krüppel homolog (Kr-h1), methyl farnesoate epoxidase (MFE), Vitellogenin (Vg). Our research identifies specific normalization thereby offering valuable insights into gene functional metabolic genetics under varying conditions. This catalog will serve resource future endeavors.
Язык: Английский
Процитировано
1
Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(49)
Опубликована: Ноя. 26, 2024
Broad complex (Br-C) and eip93F (E93) transcription factors promote insect metamorphosis from larva to pupa adult, respectively. Recently, chronologically inappropriate morphogenesis (Chinmo) has been proposed as a larval specifier in Drosophila melanogaste r. However, whether Chinmo is required for maintenance lepidopteran insects, the underlying mechanisms involved maintaining stage, its interactions with JH signaling pathway are not well understood. Here, we used binary transgenic CRISPR/Cas9 system knockout Kr-h1 (primary response gene pathway) fall armyworm (FAW). induced premature only after L5 (penultimate), whereas double L3. Sequencing differential expression (DEG) analysis of RNA isolated mutants single-cell multiome ATAC , Sf9 cells revealed that participates chromatin modifications prevent promoter accessibility promoting genes. These results suggest plays major role preventing early stages by controlling near promoters genes such Br-C E93 pupal adult development.
Язык: Английский
Процитировано
1
Fish & Shellfish Immunology, Год журнала: 2023, Номер 143, С. 109185 - 109185
Опубликована: Окт. 30, 2023
Язык: Английский
Процитировано
3