Role of Mitochondrial Dysfunction in UV‐Induced Photoaging and Skin Cancers

Experimental Dermatology, Год журнала: 2025, Номер 34(5)

Опубликована: Май 1, 2025

ABSTRACT Ultraviolet radiation (UVR) is the most detrimental external factor that induces acute photodamage, photoaging and skin cancers, with complex underlying molecular mechanisms initiated mainly by increased DNA damage reactive oxygen species (ROS) generation. Mitochondria are main organelles in cells produce ROS energy regulate various physiological pathological signalling pathways. Continuous UVR on human can induce mitochondrial mutations excessive production, creating feedback between each other subsequently causing a reduction membrane potential (MMP) respiratory capacity. Deficiencies function apoptosis, mitophagy senescence, resulting UVR‐induced photodamage photoaging. Mitochondrial biogenesis metabolic pathways play critical roles progression of particularly melanoma, which malignant infrequent type cancer. In this review, we describe recent advances determining intimate relationship damage, suggesting candidates novel chemical/natural components to protect from cancers via targeting mechanisms.

Язык: Английский

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Cosmetic Potential of Haberlea rhodopensis Extracts and Extracellular Vesicles in Human Fibroblast Cells

Cosmetics, Год журнала: 2025, Номер 12(3), С. 90 - 90

Опубликована: Май 1, 2025

Skin ageing is a complex biological process influenced by cellular senescence, oxidative stress, and extracellular matrix degradation. Emerging evidence suggests that plant-derived bioactive compounds vesicles (EVs) play crucial role in modulating homeostasis, promoting tissue regeneration, counteracting age-related morphological functional changes. This study investigates the impact of Haberlea rhodopensis vitro culture extracts, native enriched with EVs, on key processes, including morphology, mitochondrial dynamics, lysosomal activity, gene expression, genotoxicity human dermal fibroblasts. The were identified terms shape, size, morphology using dynamic light scattering, negative staining observation under transmission electron microscope. A comprehensive analysis was conducted utilizing microscopy to assess mass, fluorescence for actin cytoskeletal organization, integrity, nuclear expression profiling markers associated collagen synthesis (COL1A1, COL3A1), senescence (CDKN1A), stress response (NFE2L2). Additionally, cell cycle progression evaluated, assessed neutral comet assay. extracts EVs found preserve fibroblast enhance upregulate collagen-related expression. These effects concentration-dependent. exhibited biocompatibility minimal genotoxic effects, indicating their potential as safe agents skin rejuvenation. findings suggest enrichment hold promise cosmetic dermatological applications, particularly enhancing production, preserving mitigating alterations Further studies are warranted elucidate underlying molecular mechanisms optimize formulation strategies clinical translation.

Язык: Английский

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Novel Insights Into the Ultrastructural and Immunofluorescence Characteristics of Limb Skin in the Red‐Eared Slider Turtle (Trachemys scripta elegans)

Microscopy Research and Technique, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 7, 2024

The red-eared slider turtle, a species facing environmental challenges and habitat loss, exhibits complex skin architecture that is crucial for its adaptation survival. Our study aims to provide comprehensive characterization of the turtle's structure elucidate distribution localization various cellular components, with focus on understanding skin's role in ecological interactions. To achieve these goals, we employed light microscopy, transmission electron microscopy (TEM), immunofluorescence using 10 specific antibodies. forelimb displays large- moderate-sized scales variations color, including dark, yellow, gray hues, likely contributing camouflage protection. consists corneous material, epidermis, dermis, hypodermis. stratum basalis, spinosum, peri-corneous layer constitute three distinct layers epidermis. There are four types chromatophores, melanocytes located while melanophores, xanthophores, iridophores found within dermal layer. also well-developed peripheral nerves, blood vessels, subcutaneous muscles. Immunofluorescence staining further elucidates cells. E-cadherin CK14 strongly expressed epidermal layers, excluding material. surrounds keratinocyte cells facilitating cell-cell adhesion, present inside cells, their internal structural integrity. Sox10 CD117 identify chromatophore types, Melan-A specifically detecting only melanophores not labeling xanthophores iridophores. Tom20 used detect mitochondrial intensity skin, revealing high density mitochondria all especially compared Numerous telocytes, spindle-shaped extensions called telopods, detected dermis CD34, PDGFRα, vimentin. shows abundant myofibroblasts vascularization, numerous vessels α-SMA. This novel offers an in-depth examination limb through use antibodies, uncovering intricate interactions among components providing valuable insights into anatomical physiological adaptations. findings contribute better which may aid conservation management.

Язык: Английский

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The role of immune metabolism in skin cancers: implications for pathogenesis and therapy

Translational Cancer Research, Год журнала: 2024, Номер 13(7), С. 3898 - 3903

Опубликована: Июль 1, 2024

The skin is a complex organ that serves as critical barrier against external pathogens and environmental impact. Recent advances in immunometabolism have highlighted the intricate link between cellular metabolism immune function, particularly context of cancers. This review aims to provide comprehensive overview key metabolic pathways adaptations occur cells during homeostasis activation, explore how reprogramming contributes pathogenesis specific We discuss interplay tumor infiltrating cells, which shapes microenvironment influences disease outcomes. delves into role various pathways, such glycolysis, oxidative phosphorylation, lipid metabolism, regulation cell function their impact on development progression Furthermore, we examine potential targeting therapeutic strategy cancers challenges future perspectives this rapidly evolving field. By understanding basis responses, can develop novel, personalized therapies for treatment cancers, ultimately improving patient outcomes quality life. insights gained from will contribute growing body knowledge its application management paving way more effective targeted interventions future.

Язык: Английский

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