Leg immobilization and subsequent recovery resistance training affect skeletal muscle angiogenesis related markers in young healthy adults regardless of prior resistance training experience DOI Creative Commons
Mason C. McIntosh, J. Max Michel, Joshua S. Godwin

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 26, 2024

ABSTRACT We recently reported that resistance trained (T, n=10) and untrained (UT, n=11) young adults experience vastus lateralis (VL) muscle atrophy following two weeks of disuse, 8 recovery training (RT) promotes VL hypertrophy in both participant cohorts. However, angiogenesis targets capillary number were not examined currently no human studies have sought to determine if disuse followed by RT affects these outcomes. Thus, we whether and/or affected All participants underwent left leg immobilization using locking braces crutches eight (3d/week) knee extensor focused progressive RT. biopsies obtained at baseline (PRE), immediately after (MID), (POST). Western blotting was used assay markers immunohistochemistry performed 16/21 type I II fiber number. Significant main effects time (p<0.05) observed for protein levels VEGF (MID<POST), VEGFR2 (PRE&MID<POST), TSP-1 (PRE<POST), TIMP1 phosphorylated/pan eNOS (Ser1177) (POST<PRE), pan (PRE<POST). exhibited a status*time (p=0.018), but differences existed between T UT any point. A significant effect cross-sectional area (fCSA) increased from MID POST (+25%, p<0.001) PRE (+20%, p=0.019). No correlations exist percentage changes fCSA PRE-to-MID (r= 0.020), MID-to-POST 0.392), or PRE-to-POST −0.120) across all (p>0.100). Although affect skeletal angiogenesis-related targets, prior history does differentially NEW AND NOTEWORTHY This is the first study examine how limb molecular outcomes related younger with without history. Regardless history, responses are largely similar cohorts suggestive reduced (pre-mid) (mid-post) angiogenic response, subsequent training.

Язык: Английский

The Impact of a Western Diet and Resistance Training in a Rat Model of Mammary Cancer DOI Creative Commons
Jéssica Silva, Tiago Azevedo, Rita Ferreira

и другие.

Life, Год журнала: 2025, Номер 15(2), С. 250 - 250

Опубликована: Фев. 6, 2025

This study aimed to investigate the impact of a Western diet and resistance training on cardiac remodeling in rat model chemically induced mammary cancer. Fifty-six female Wistar rats were randomly assigned one eight experimental groups, evaluating standard diets, exercise sedentarism, induction Mammary cancer was via intraperitoneal administration N-methyl-N-nitrosourea (MNU) (50 mg/kg) at seven weeks age. The protocol consisted ladder climbing three times per week for an 18-week period, with gradual increase load over time. At end 20-week animals anesthetized underwent echocardiography. Subsequently, euthanized, organs visceral adipose tissue (VAT) collected analyzed. A histopathological examination performed tumors. increased relative VAT contributed cardiovascular tumor-related changes, including interventricular septum thickness (IVS) left ventricle posterior wall (LVPW) end-systole. Exercise reduced fat accumulation, improved performance, helped regulate function, as indicated by higher eccentricity index (EI) WD+EX group compared WD group. associated accumulation initially delayed tumor initiation; however, time, it bigger aggressiveness. also initiation but LVPW. Exercise, when combined WD, accelerated tumorigenesis, malignant transformation invasiveness, resulted prevalence invasive These findings underscore complex potentially compounding effects progression.

Язык: Английский

Процитировано

0
The effect of resistance training on blood pressure and resting heart rate in type 2 diabetes: A systematic review and meta-analysis of randomized controlled trials DOI
Ruei‐Lung Lin,

Kebing Yang,

Hengtao Guo

и другие.

Diabetes Research and Clinical Practice, Год журнала: 2025, Номер unknown, С. 112016 - 112016

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Resistance Exercise in Treating Heart Failure with Preserved Ejection Fraction (HFpEF) and Obesity: Targeting Skeletal Muscle Abnormalities and Ectopic Adipose Depots DOI Creative Commons
Daniel J. McDonough

Physiologia, Год журнала: 2025, Номер 5(1), С. 10 - 10

Опубликована: Фев. 27, 2025

Heart failure is a leading cause of morbidity and mortality worldwide [...]

Язык: Английский

Процитировано

0
Network-based modelling reveals cell-type enriched patterns of non-coding RNA regulation during human skeletal muscle remodelling DOI Creative Commons
Jonathan C. Mcleod, Changhyun Lim, Tanner Stokes

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 11, 2024

ABSTRACT A majority of human genes produce non-protein-coding RNA (ncRNA), and some have roles in development disease. Neither ncRNA nor skeletal muscle is ideally studied using short-read sequencing, so we used a customised pipeline network modelling to study cell-type specific responses during growth at scale. We completed five resistance-training studies (n=144 subjects), identifying 61% who successfully accrued muscle-mass. produced 288 transcriptome-wide profiles found 110 ncRNAs linked vivo, while transcriptome-driven model demonstrated interactions via number discrete functional pathways single-cell types. This analysis included established hypertrophy-related ncRNAs, including CYTOR – which was leukocyte-associated (FDR = 4.9 x10 -7 ). Novel hypertrophy-linked PPP1CB-DT (myofibril assembly genes, FDR 8.15 x 10 -8 ), EEF1A1P24 TMSB4XP8 (vascular remodelling angiogenesis 2.77 -5 also discovered that hypertrophy lncRNA MYREM shows myonuclear expression pattern vivo . Our multi-layered analyses single-cell-associated are identifiable from bulk transcriptomic data mediate their association with multiple cell types set interacting pathways. One Sentence Summary an optimised strategy identify regulated one hundred forty-four people, spatial imaging providing biological context.

Язык: Английский

Процитировано

1
Network-based modelling reveals cell-type enriched patterns of non-coding RNA regulation during human skeletal muscle remodelling DOI Creative Commons
Jonathan C. Mcleod, Changhyun Lim, Tanner Stokes

и другие.

Deleted Journal, Год журнала: 2024, Номер 1(4)

Опубликована: Окт. 1, 2024

Abstract A majority of human genes produce non-protein-coding RNA (ncRNA), and some have roles in development disease. Neither ncRNA nor skeletal muscle is ideally studied using short-read sequencing, so we used a customized pipeline network modelling to study cell-type specific responses during growth at scale. We completed five resistance-training studies (n = 144 subjects), identifying 61% who successfully accrued muscle-mass. produced 288 transcriptome-wide profiles found 110 ncRNAs linked vivo, while transcriptome-driven model demonstrated interactions via number discrete functional pathways single-cell types. This analysis included established hypertrophy-related ncRNAs, including CYTOR—which was leukocyte-associated (false discovery rate [FDR] 4.9 × 10−7). Novel hypertrophy-linked PPP1CB-DT (myofibril assembly genes, FDR 8.15 10−8), EEF1A1P24 TMSB4XP8 (vascular remodelling angiogenesis 2.77 10−5). also discovered that hypertrophy lncRNA MYREM shows myonuclear expression pattern vivo. Our multi-layered analyses single-cell-associated are identifiable from bulk transcriptomic data mediate their association with multiple cell types set interacting pathways.

Язык: Английский

Процитировано

1
Effects of wearing myopia glasses on eye movement and scleral blood supply DOI

Lyuqi Tan,

Jilin Tan,

Heping Yang

и другие.

Medicine International, Год журнала: 2024, Номер 4(6)

Опубликована: Июль 18, 2024

The present study examined the effect of wearing myopia glasses on eye movement and scleral blood supply. For this purpose, a total 30 individuals were recruited for self-control study. Under same fixation distance, wore 0.00 D -10.00 glasses. amount generated when shifting from gazing at central point to light source located left or right was measured compared between two results revealed that range significantly reduced after When point, difference rotation distances -10.0 0.73±0.45 mm (t=8.93, P<0.01) 0.73±0.43 (t=9.34, P<0.01). Similar obtained viewed shift in gaze point. On whole, demonstrates concave lenses limits eyeball movement. Restricted can affect vascular changes within extraocular muscles flow, thereby affecting supply anterior segment sclera eye, potentially accelerating development myopia.

Язык: Английский

Процитировано

0
Leg immobilization and subsequent recovery resistance training affect skeletal muscle angiogenesis related markers in young healthy adults regardless of prior resistance training experience DOI Creative Commons
Mason C. McIntosh, J. Max Michel, Joshua S. Godwin

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 26, 2024

ABSTRACT We recently reported that resistance trained (T, n=10) and untrained (UT, n=11) young adults experience vastus lateralis (VL) muscle atrophy following two weeks of disuse, 8 recovery training (RT) promotes VL hypertrophy in both participant cohorts. However, angiogenesis targets capillary number were not examined currently no human studies have sought to determine if disuse followed by RT affects these outcomes. Thus, we whether and/or affected All participants underwent left leg immobilization using locking braces crutches eight (3d/week) knee extensor focused progressive RT. biopsies obtained at baseline (PRE), immediately after (MID), (POST). Western blotting was used assay markers immunohistochemistry performed 16/21 type I II fiber number. Significant main effects time (p<0.05) observed for protein levels VEGF (MID<POST), VEGFR2 (PRE&MID<POST), TSP-1 (PRE<POST), TIMP1 phosphorylated/pan eNOS (Ser1177) (POST<PRE), pan (PRE<POST). exhibited a status*time (p=0.018), but differences existed between T UT any point. A significant effect cross-sectional area (fCSA) increased from MID POST (+25%, p<0.001) PRE (+20%, p=0.019). No correlations exist percentage changes fCSA PRE-to-MID (r= 0.020), MID-to-POST 0.392), or PRE-to-POST −0.120) across all (p>0.100). Although affect skeletal angiogenesis-related targets, prior history does differentially NEW AND NOTEWORTHY This is the first study examine how limb molecular outcomes related younger with without history. Regardless history, responses are largely similar cohorts suggestive reduced (pre-mid) (mid-post) angiogenic response, subsequent training.

Язык: Английский

Процитировано

0