Leg immobilization and subsequent recovery resistance training affect skeletal muscle angiogenesis related markers in young healthy adults regardless of prior resistance training experience DOI Creative Commons
Mason C. McIntosh, J. Max Michel, Joshua S. Godwin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

ABSTRACT We recently reported that resistance trained (T, n=10) and untrained (UT, n=11) young adults experience vastus lateralis (VL) muscle atrophy following two weeks of disuse, 8 recovery training (RT) promotes VL hypertrophy in both participant cohorts. However, angiogenesis targets capillary number were not examined currently no human studies have sought to determine if disuse followed by RT affects these outcomes. Thus, we whether and/or affected All participants underwent left leg immobilization using locking braces crutches eight (3d/week) knee extensor focused progressive RT. biopsies obtained at baseline (PRE), immediately after (MID), (POST). Western blotting was used assay markers immunohistochemistry performed 16/21 type I II fiber number. Significant main effects time (p<0.05) observed for protein levels VEGF (MID<POST), VEGFR2 (PRE&MID<POST), TSP-1 (PRE<POST), TIMP1 phosphorylated/pan eNOS (Ser1177) (POST<PRE), pan (PRE<POST). exhibited a status*time (p=0.018), but differences existed between T UT any point. A significant effect cross-sectional area (fCSA) increased from MID POST (+25%, p<0.001) PRE (+20%, p=0.019). No correlations exist percentage changes fCSA PRE-to-MID (r= 0.020), MID-to-POST 0.392), or PRE-to-POST −0.120) across all (p>0.100). Although affect skeletal angiogenesis-related targets, prior history does differentially NEW AND NOTEWORTHY This is the first study examine how limb molecular outcomes related younger with without history. Regardless history, responses are largely similar cohorts suggestive reduced (pre-mid) (mid-post) angiogenic response, subsequent training.

Language: Английский

The Impact of a Western Diet and Resistance Training in a Rat Model of Mammary Cancer DOI Creative Commons
Jéssica Silva, Tiago Azevedo, Rita Ferreira

et al.

Life, Journal Year: 2025, Volume and Issue: 15(2), P. 250 - 250

Published: Feb. 6, 2025

This study aimed to investigate the impact of a Western diet and resistance training on cardiac remodeling in rat model chemically induced mammary cancer. Fifty-six female Wistar rats were randomly assigned one eight experimental groups, evaluating standard diets, exercise sedentarism, induction Mammary cancer was via intraperitoneal administration N-methyl-N-nitrosourea (MNU) (50 mg/kg) at seven weeks age. The protocol consisted ladder climbing three times per week for an 18-week period, with gradual increase load over time. At end 20-week animals anesthetized underwent echocardiography. Subsequently, euthanized, organs visceral adipose tissue (VAT) collected analyzed. A histopathological examination performed tumors. increased relative VAT contributed cardiovascular tumor-related changes, including interventricular septum thickness (IVS) left ventricle posterior wall (LVPW) end-systole. Exercise reduced fat accumulation, improved performance, helped regulate function, as indicated by higher eccentricity index (EI) WD+EX group compared WD group. associated accumulation initially delayed tumor initiation; however, time, it bigger aggressiveness. also initiation but LVPW. Exercise, when combined WD, accelerated tumorigenesis, malignant transformation invasiveness, resulted prevalence invasive These findings underscore complex potentially compounding effects progression.

Language: Английский

Citations

0
The effect of resistance training on blood pressure and resting heart rate in type 2 diabetes: A systematic review and meta-analysis of randomized controlled trials DOI
Ruei‐Lung Lin,

Kebing Yang,

Hengtao Guo

et al.

Diabetes Research and Clinical Practice, Journal Year: 2025, Volume and Issue: unknown, P. 112016 - 112016

Published: Feb. 1, 2025

Language: Английский

Citations

0
Resistance Exercise in Treating Heart Failure with Preserved Ejection Fraction (HFpEF) and Obesity: Targeting Skeletal Muscle Abnormalities and Ectopic Adipose Depots DOI Creative Commons
Daniel J. McDonough

Physiologia, Journal Year: 2025, Volume and Issue: 5(1), P. 10 - 10

Published: Feb. 27, 2025

Heart failure is a leading cause of morbidity and mortality worldwide [...]

Language: Английский

Citations

0
Network-based modelling reveals cell-type enriched patterns of non-coding RNA regulation during human skeletal muscle remodelling DOI Creative Commons
Jonathan C. Mcleod, Changhyun Lim, Tanner Stokes

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 11, 2024

ABSTRACT A majority of human genes produce non-protein-coding RNA (ncRNA), and some have roles in development disease. Neither ncRNA nor skeletal muscle is ideally studied using short-read sequencing, so we used a customised pipeline network modelling to study cell-type specific responses during growth at scale. We completed five resistance-training studies (n=144 subjects), identifying 61% who successfully accrued muscle-mass. produced 288 transcriptome-wide profiles found 110 ncRNAs linked vivo, while transcriptome-driven model demonstrated interactions via number discrete functional pathways single-cell types. This analysis included established hypertrophy-related ncRNAs, including CYTOR – which was leukocyte-associated (FDR = 4.9 x10 -7 ). Novel hypertrophy-linked PPP1CB-DT (myofibril assembly genes, FDR 8.15 x 10 -8 ), EEF1A1P24 TMSB4XP8 (vascular remodelling angiogenesis 2.77 -5 also discovered that hypertrophy lncRNA MYREM shows myonuclear expression pattern vivo . Our multi-layered analyses single-cell-associated are identifiable from bulk transcriptomic data mediate their association with multiple cell types set interacting pathways. One Sentence Summary an optimised strategy identify regulated one hundred forty-four people, spatial imaging providing biological context.

Language: Английский

Citations

1
Network-based modelling reveals cell-type enriched patterns of non-coding RNA regulation during human skeletal muscle remodelling DOI Creative Commons
Jonathan C. Mcleod, Changhyun Lim, Tanner Stokes

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 1(4)

Published: Oct. 1, 2024

Abstract A majority of human genes produce non-protein-coding RNA (ncRNA), and some have roles in development disease. Neither ncRNA nor skeletal muscle is ideally studied using short-read sequencing, so we used a customized pipeline network modelling to study cell-type specific responses during growth at scale. We completed five resistance-training studies (n = 144 subjects), identifying 61% who successfully accrued muscle-mass. produced 288 transcriptome-wide profiles found 110 ncRNAs linked vivo, while transcriptome-driven model demonstrated interactions via number discrete functional pathways single-cell types. This analysis included established hypertrophy-related ncRNAs, including CYTOR—which was leukocyte-associated (false discovery rate [FDR] 4.9 × 10−7). Novel hypertrophy-linked PPP1CB-DT (myofibril assembly genes, FDR 8.15 10−8), EEF1A1P24 TMSB4XP8 (vascular remodelling angiogenesis 2.77 10−5). also discovered that hypertrophy lncRNA MYREM shows myonuclear expression pattern vivo. Our multi-layered analyses single-cell-associated are identifiable from bulk transcriptomic data mediate their association with multiple cell types set interacting pathways.

Language: Английский

Citations

1
Effects of wearing myopia glasses on eye movement and scleral blood supply DOI

Lyuqi Tan,

Jilin Tan,

Heping Yang

et al.

Medicine International, Journal Year: 2024, Volume and Issue: 4(6)

Published: July 18, 2024

The present study examined the effect of wearing myopia glasses on eye movement and scleral blood supply. For this purpose, a total 30 individuals were recruited for self-control study. Under same fixation distance, wore 0.00 D -10.00 glasses. amount generated when shifting from gazing at central point to light source located left or right was measured compared between two results revealed that range significantly reduced after When point, difference rotation distances -10.0 0.73±0.45 mm (t=8.93, P<0.01) 0.73±0.43 (t=9.34, P<0.01). Similar obtained viewed shift in gaze point. On whole, demonstrates concave lenses limits eyeball movement. Restricted can affect vascular changes within extraocular muscles flow, thereby affecting supply anterior segment sclera eye, potentially accelerating development myopia.

Language: Английский

Citations

0
Leg immobilization and subsequent recovery resistance training affect skeletal muscle angiogenesis related markers in young healthy adults regardless of prior resistance training experience DOI Creative Commons
Mason C. McIntosh, J. Max Michel, Joshua S. Godwin

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

ABSTRACT We recently reported that resistance trained (T, n=10) and untrained (UT, n=11) young adults experience vastus lateralis (VL) muscle atrophy following two weeks of disuse, 8 recovery training (RT) promotes VL hypertrophy in both participant cohorts. However, angiogenesis targets capillary number were not examined currently no human studies have sought to determine if disuse followed by RT affects these outcomes. Thus, we whether and/or affected All participants underwent left leg immobilization using locking braces crutches eight (3d/week) knee extensor focused progressive RT. biopsies obtained at baseline (PRE), immediately after (MID), (POST). Western blotting was used assay markers immunohistochemistry performed 16/21 type I II fiber number. Significant main effects time (p<0.05) observed for protein levels VEGF (MID<POST), VEGFR2 (PRE&MID<POST), TSP-1 (PRE<POST), TIMP1 phosphorylated/pan eNOS (Ser1177) (POST<PRE), pan (PRE<POST). exhibited a status*time (p=0.018), but differences existed between T UT any point. A significant effect cross-sectional area (fCSA) increased from MID POST (+25%, p<0.001) PRE (+20%, p=0.019). No correlations exist percentage changes fCSA PRE-to-MID (r= 0.020), MID-to-POST 0.392), or PRE-to-POST −0.120) across all (p>0.100). Although affect skeletal angiogenesis-related targets, prior history does differentially NEW AND NOTEWORTHY This is the first study examine how limb molecular outcomes related younger with without history. Regardless history, responses are largely similar cohorts suggestive reduced (pre-mid) (mid-post) angiogenic response, subsequent training.

Language: Английский

Citations

0