CRISPR/Cas9-targeted mutagenesis of OsERA1 confers enhanced responses to abscisic acid and drought stress and increased primary root growth under nonstressed conditions in rice

PLoS ONE, Год журнала: 2020, Номер 15(12), С. e0243376 - e0243376

Опубликована: Дек. 3, 2020

Abscisic acid (ABA) signaling components play an important role in the drought stress response plants. Arabidopsis thaliana ENHANCED RESPONSE TO ABA1 ( ERA1 ) encodes β-subunit of farnesyltransferase and regulates ABA dehydration response. Therefore, is candidate gene for enhancing tolerance numerous crops. However, a rice Oryza sativa homolog has not been characterized previously. Here, we show that osera1 mutant lines, harboring CRISPR/Cas9-induced frameshift mutations, exhibit similar leaf growth as control plants but increased primary root growth. The lines also display sensitivity to enhanced through stomatal regulation. These results illustrate OsERA1 negative regulator under nonstressed conditions responses rice. findings improve our understanding suggest strategy genetically

Язык: Английский

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CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors

Genome biology, Год журнала: 2019, Номер 20(1)

Опубликована: Апрель 29, 2019

Increasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based platform in plants evolve the rice (Oryza sativa) SF3B1 spliceosomal protein for resistance splicing inhibitors. mutant variants, termed SF3B1-GEX1A-Resistant (SGR), confer variable levels of Studies structural basis inhibitor binding SGRs corroborate phenotype. This can be used interrogate molecular functions key biomolecules engineer traits improved performance adaptation under climate change conditions.

Язык: Английский

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Effect of Heavy Metals on Plant Growth: An Overview

Springer eBooks, Год журнала: 2020, Номер unknown, С. 79 - 101

Опубликована: Янв. 1, 2020

Язык: Английский

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Engineering Multiple Abiotic Stress Tolerance in Canola, Brassica napus

Frontiers in Plant Science, Год журнала: 2020, Номер 11

Опубликована: Фев. 25, 2020

Impacts of climate change like global warming, drought, flooding, and other extreme events are posing severe challenges to crop production. Contribution Brassica napus towards the oilseed industry makes it an essential component international trade agroeconomics. Consequences from increasing occurrences multiple abiotic stresses on this leading agroeconomic losses making vital endow B. with ability survive maintain yield when faced simultaneous exposure stresses. For improved understanding stress sensing machinery, there is a need for analyzing regulatory pathways stress-responsive genes elements such as non-coding RNAs. However, our these their interactions in far complete. This review outlines current knowledge role imparting tolerance napus. Analysis network crosstalk through omics data mining now possible unravel underlying complexity required signalling plants. Novel biotechnological approaches transgene-free genome editing utilization nanoparticles gene delivery tools also discussed. These can contribute providing solutions developing resilient varieties reduced limitations. The potential synthetic biology engineer modify networks fine-tuning plant responses adaption highlighted.

Язык: Английский

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In planta gene targeting can be enhanced by the use of CRISPR/Cas12a

The Plant Journal, Год журнала: 2019, Номер 100(5), С. 1083 - 1094

Опубликована: Авг. 5, 2019

The controlled change of plant genomes by homologous recombination (HR) is still difficult to achieve. We previously developed the in planta gene targeting (ipGT) technology which depends on simultaneous activation target locus a double-strand break and excision vector. Whereas use SpCas9 resulted low ipGT frequencies Arabidopsis, we were recently able improve efficiency using egg cell-specific expression potent but less broadly applicable SaCas9 nuclease. In this study, now tested whether could further, either performing it cells with enhanced intrachromosomal HR efficiencies or Cas12a, different kind CRISPR/Cas nuclease an alternative cutting mechanism. show before that plants possess three kinds DNA ATPase complexes, all lead instabilities genomic repeats if lost mutation. As these proteins act independent pathways, double mutants 20-80-fold. However, not obtain higher frequencies, indicating mechanisms for (GT) chromosomal repeat-induced differ. LbCas12a, GT than SaCas9, despite lower non-homologous end-joining (NHEJ) induction efficiency, demonstrating particular suitability Cas12a induce HR. has substantial restrictions due its longer GC rich PAM sequence, LbCas12a AT-rich broadens range drastically, particularly when CG-deserts like promoters introns.

Язык: Английский

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CRISPR-Mediated Engineering across the Central Dogma in Plant Biology for Basic Research and Crop Improvement

Molecular Plant, Год журнала: 2020, Номер 14(1), С. 127 - 150

Опубликована: Ноя. 3, 2020

The central dogma (CD) of molecular biology is the transfer genetic information from DNA to RNA protein. Major CD processes governing flow include cell cycle, replication, chromosome packaging, epigenetic changes, transcription, posttranscriptional alterations, translation, and posttranslational modifications. are tightly regulated in plants maintain integrity throughout life cycle pass materials next generation. Engineering various involved gene regulation will accelerate crop improvement feed growing world population. CRISPR technology enables programmable editing alter DNA, RNA, or protein, which would have been impossible past. Here, an overview recent advancements tool development CRISPR-based modulations that expedite basic applied plant research provided. Furthermore, applications major thriving areas research, such as discovery (allele mining cryptic activation), introgression (de novo domestication haploid induction), application desired traits beneficial farmers consumers (biotic/abiotic stress-resilient crops, factories, delayed senescence), described. Finally, global regulatory policies, challenges, prospects for CRISPR-mediated discussed.

Язык: Английский

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CRISPR/Cas9-Mediated Multi-Allelic Gene Targeting in Sugarcane Confers Herbicide Tolerance

Frontiers in Genome Editing, Год журнала: 2021, Номер 3

Опубликована: Июль 8, 2021

Sugarcane is the source of 80% sugar and 26% bioethanol produced globally. However, its complex, highly polyploid genome (2

Язык: Английский

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Transgenic Crops for Biofortification

Frontiers in Sustainable Food Systems, Год журнала: 2020, Номер 4

Опубликована: Ноя. 3, 2020

Global Food Security concerns impact greatly on the United Nation's Sustainable Development Goals (SDGs) which are heavily focused removing hunger by 2030. The Index (GFSI) of 2017 has reported a reduction in global food security over past year, after four years consecutive gains. This been mainly due to forced migration, climate change and political instability many countries. Since nutrition is one main factors maintaining healthy lifestyle meeting requirements security, several national surveys conducted various countries have provided an avenue for governments assess malnutrition problems across population. For example, National Nutrition Survey carried out 2011 2018 Pakistan indicated around 50% population be insecure based nutritional status available food. survey also highlighted acute deficiency micronutrients diet resulting disorders, especially among female In view these facts, efforts being made globally enhance value our agricultural products staple crops using biotechnological approaches. Biofortification generally referred as their with enhanced value. can achieved either conventional selective breeding or through genetic engineering. differs from fortification because it focuses make plant foods naturally more nutritive rather than adding nutrient supplements during processing.

Язык: Английский

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Engineering Host Microbiome for Crop Improvement and Sustainable Agriculture

Frontiers in Microbiology, Год журнала: 2021, Номер 12

Опубликована: Май 28, 2021

Dynamic consortium of microbial communities (bacteria, fungi, protists, viruses, and nematodes) colonizing multiple tissue types coevolving conclusively with the host plant is designated as a microbiome. The interplay between its mutualists supports several agronomic functions, establishing crucial role in beneficial activities. Deeper functional mechanistic understanding plant-microbial ecosystems will render many “ecosystem services” by emulating symbiotic interactions plants, soil, microbes for enhanced productivity sustainability. Therefore, microbiome engineering represents an emerging biotechnological tool to directly add, remove, or modify properties higher specificity efficacy. main goal enhancement functions such biotic/abiotic stresses, fitness productivities, etc. Various ecological-, biochemical-, molecular-based approaches have come up new paradigm disentangling microbiome-based agromanagement hurdles. Furthermore, multidisciplinary provide predictive framework achieving reliable sustainably engineered plant-microbiome stress physiology, nutrient recycling, high-yielding disease-resistant genotypes.

Язык: Английский

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Genome Editing Technologies for Rice Improvement: Progress, Prospects, and Safety Concerns

Frontiers in Genome Editing, Год журнала: 2020, Номер 2

Опубликована: Июнь 4, 2020

Rice (Oryza sativa) is an important staple food crop worldwide; to meet the growing nutritional requirements of increasing population in face climate change, qualitative and quantitative traits rice need be improved. Stress-tolerant varieties must developed with stable or higher yields under stress conditions. Genome editing speed breeding have improved accuracy pace breeding. New technologies including genome been established rice, expanding potential for improvement. Recently, other techniques such as CRISPR-directed evolution, CRISPR-Cas12a, base editors also used efficient rice. Since excellent model system functional studies due its small close syntenic relationships cereal crops, new genome-editing continue use In this review, we focus on tools improvement address current challenges provide examples We shed light scope systems delivering homology-directed repair templates. Finally, discuss safety concerns methods obtaining transgene-free crops.

Язык: Английский

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