Contributions of Adaptive Laboratory Evolution towards the Enhancement of the Biotechnological Potential of Non-Conventional Yeast Species DOI Creative Commons
Ticiana Fernandes, Carolina Osório, Maria João Sousa

и другие.

Journal of Fungi, Год журнала: 2023, Номер 9(2), С. 186 - 186

Опубликована: Янв. 31, 2023

Changes in biological properties over several generations, induced by controlling short-term evolutionary processes the laboratory through selective pressure, and whole-genome re-sequencing, help determine genetic basis of microorganism's adaptive evolution (ALE). Due to versatility this technique imminent urgency for alternatives petroleum-based strategies, ALE has been actively conducted yeasts, primarily using conventional species Saccharomyces cerevisiae, but also non-conventional yeasts. As a hot topic at moment since genetically modified organisms are debatable subject global consensus on their employment not yet attained, panoply new studies employing approaches have emerged many different applications exploited context. In present review, we gathered, first time, relevant showing yeast towards biotechnological improvement, cataloging them according aim study, comparing considering used, outcome experiment, employed methodology. This review sheds light applicability as powerful tool enhance features improve performance biotechnology, with emphasis species, an alternative or combination genome editing approaches.

Язык: Английский

Valorization of Starch to Biobased Materials: A Review DOI Open Access
Kehinde James Falua,

Anamol Pokharel,

Amin Babaei‐Ghazvini

и другие.

Polymers, Год журнала: 2022, Номер 14(11), С. 2215 - 2215

Опубликована: Май 30, 2022

Many concerns are being expressed about the biodegradability, biocompatibility, and long-term viability of polymer-based substances. This prompted quest for an alternative source material that could be utilized various purposes. Starch is widely used as a thickener, emulsifier, binder in many food non-food sectors, but research focuses on increasing its application beyond these areas. Due to low cost, renewability, abundance, starch considered "green path" raw generating porous substances such aerogels, biofoams, bioplastics, which have sparked academic interest. Existing has focused strategies developing biomaterials from organic polymers (e.g., cellulose), there been little polysaccharide counterpart (starch). review paper highlighted structure starch, context amylose amylopectin, extraction modification with their processes limitations. Moreover, this describes nanofillers, intelligent pH-sensitive films, aerogels types, precursors, including drying manufacturing. The perspectives reveal great potential starch-based food, pharmaceuticals, biomedicine, applications.

Язык: Английский

Процитировано

61

Sustainable use of CRISPR/Cas in fish aquaculture: the biosafety perspective DOI Creative Commons
Arinze S. Okoli, Torill Blix, Anne Ingeborg Myhr

и другие.

Transgenic Research, Год журнала: 2021, Номер 31(1), С. 1 - 21

Опубликована: Июль 25, 2021

Abstract Aquaculture is becoming the primary source of seafood for human diets, and farmed fish aquaculture one its fastest growing sectors. The industry currently faces several challenges including infectious parasitic diseases, reduced viability, fertility reduction, slow growth, escapee environmental pollution. commercialization growth-enhanced AquAdvantage salmon CRISPR/Cas9-developed tilapia ( Oreochromis niloticus ) proffers genetic engineering genome editing tools, e.g. CRISPR/Cas, as potential solutions to these challenges. Future traits being developed in different species include disease resistance, sterility, enhanced growth. Despite notable advances, off-target effect non-clarification trait-related genes among other technical hinder full realization CRISPR/Cas potentials breeding. In addition, current regulatory risk assessment frameworks are not fit-for purpose regarding notwithstanding that public acceptance key products new technology. this study, we discuss how can be used overcome some limitations focusing on diseases release aquaculture. We further present limitations, use proffer research strategies will provide much-needed data decisions, assessments, increased awareness sustainable applications with emphasis Atlantic Salmo salar

Язык: Английский

Процитировано

59

Host plant resistance for fall armyworm management in maize: relevance, status and prospects in Africa and Asia DOI Creative Commons
B. M. Prasanna, Anna Bruce, Yoseph Beyene

и другие.

Theoretical and Applied Genetics, Год журнала: 2022, Номер 135(11), С. 3897 - 3916

Опубликована: Март 23, 2022

Abstract Key message Sustainable control of fall armyworm (FAW) requires implementation effective integrated pest management (IPM) strategies, with host plant resistance as a key component. Significant opportunities exist for developing and deploying elite maize cultivars native genetic and/or transgenic FAW in both Africa Asia. The [ Spodoptera frugiperda (J.E. Smith); FAW] has emerged serious since 2016 Africa, 2018 Asia, affecting the food security livelihoods millions smallholder farmers, especially those growing maize. which is one components. strides have been made breeding lines hybrids to based on strong foundation insect-resistant tropical germplasm developed at International Maize Wheat Improvement Center, Mexico. These efforts are further intensified develop deploy tolerance/resistance farmer-preferred traits suitable diverse agro-ecologies Independently, genetically modified Bt already commercialized South few countries Asia (Philippines Vietnam), while being commercialize events additional In where commercialized, it important implement robust insect strategy. Combinations also need be explored path more sustainable options. We highlight critical gaps priorities research development maize, particularly context

Язык: Английский

Процитировано

59

Future of Bacterial Disease Management in Crop Production DOI
Anuj Sharma, Peter Abrahamian, Renato Carvalho

и другие.

Annual Review of Phytopathology, Год журнала: 2022, Номер 60(1), С. 259 - 282

Опубликована: Июль 6, 2022

Bacterial diseases are a constant threat to crop production globally. Current management strategies rely on an array of tactics, including improved cultural practices; application bactericides, plant activators, and biocontrol agents; use resistant varieties when available. However, effective remains challenge, as the longevity deployed tactics is threatened by constantly changing bacterial populations. Increased scrutiny impact pesticides human environmental health underscores need for alternative solutions that durable, sustainable, accessible farmers, environmentally friendly. In this review, we discuss strengths shortcomings existing practices dissect recent advances may shape future disease management. We conclude resistance through genome modification be most arsenal against diseases. Nonetheless, more research necessary developing novel meet food demand growing global population.

Язык: Английский

Процитировано

55

Genome editing around the globe: An update on policies and perceptions DOI Creative Commons
Thorben Sprink, Ralf Wilhelm, Frank Hartung

и другие.

PLANT PHYSIOLOGY, Год журнала: 2022, Номер 190(3), С. 1579 - 1587

Опубликована: Авг. 17, 2022

A decade ago, the CRISPR/Cas system has been adapted for genome editing. Since then, hundreds of organisms have altered using editing and discussions were raised on regulatory status edited esp. crops. To date, many countries made decisions products editing, by exempting some kinds edits from classical GMO regulation. However, guidance differs between even in same region. Several are still debating issue or progress updating systems to cover The current global situation different is putting a harmonized framework genome-edited crops far future. In this update, we summarize developments field regulation concerning present short insight into perception society.

Язык: Английский

Процитировано

55

Genome Editing for Sustainable Crop Improvement and Mitigation of Biotic and Abiotic Stresses DOI Creative Commons
Mohd Fadhli Hamdan,

Chou Khai Soong Karlson,

Ee Yang Teoh

и другие.

Plants, Год журнала: 2022, Номер 11(19), С. 2625 - 2625

Опубликована: Окт. 6, 2022

Climate change poses a serious threat to global agricultural activity and food production. Plant genome editing technologies have been widely used develop crop varieties with superior qualities or can tolerate adverse environmental conditions. Unlike conventional breeding techniques (e.g., selective mutation breeding), modern tools offer more targeted specific alterations of the plant could significantly speed up progress developing crops desired traits, such as higher yield and/or stronger resilience changing environment. In this review, we discuss current development future applications in mitigating impacts biotic abiotic stresses on agriculture. We focus specifically CRISPR/Cas system, which has center attention last few years revolutionary genome-editing tool various species. also conducted bibliographic analysis CRISPR-related papers published from 2012 2021 (10 years) identify trends potential CRISPR/Cas-related research. addition, review article outlines shortcomings challenges employing agriculture notes prospective. believe combining innovative would be key optimizing improvement beyond limitations traditional practices.

Язык: Английский

Процитировано

53

Genetically modified foods: bibliometric analysis on consumer perception and preference DOI Creative Commons

R Sendhil,

Joan Nyika,

Sheel Yadav

и другие.

GM crops & food, Год журнала: 2022, Номер 13(1), С. 65 - 85

Опубликована: Апрель 11, 2022

In this study, we present the bibliometric trends emerging from research outputs on consumer perception and preference for genetically modified (GM) foods policy prescriptions enabling consumption using VOSviewer visualization software. Consumers' positive response is largely influenced by decision of governments to ban or approve GM crops cultivation. Similarly, public support increases when potential benefits technology are well articulated, with a price discount, people's trust government belief in science influence media. Europe USA first region country, respectively, terms number active institutions per output, per-capita GDP publication citations. We suggest research-, agri-food industries-, society-oriented policies be implemented stakeholders ensure safety foods, encourage consumer-based studies, increase awareness toward these food products.

Язык: Английский

Процитировано

49

Green Revolution to Gene Revolution: Technological Advances in Agriculture to Feed the World DOI Creative Commons
Mohd Fadhli Hamdan, Siti Nurfadhlina Mohd Noor, Nazrin Abd-Aziz

и другие.

Plants, Год журнала: 2022, Номер 11(10), С. 1297 - 1297

Опубликована: Май 12, 2022

Technological applications in agriculture have evolved substantially to increase crop yields and quality meet global food demand. Conventional techniques, such as seed saving, selective breeding, mutation breeding (variation breeding), dramatically increased production, especially during the 'Green Revolution' 1990s. However, newer issues, limited arable lands, climate change, ever-increasing demand, pose challenges agricultural production threaten security. In following 'Gene era, rapid innovations biotechnology field provide alternative strategies further improve yield, quality, resilience towards biotic abiotic stresses. These include introduction of DNA recombinant technology genome editing transcription activator-like effector (TALEN), zinc-finger nucleases (ZFN), clustered regularly interspaced short palindromic repeats/CRISPR associated (CRISPR/Cas) systems. acceptance future these modern tools rely on regulatory frameworks governing their development various countries. Herein, we examine evolution technological agriculture, focusing motivations for introduction, technical challenges, possible benefits concerns, genetically engineered product production.

Язык: Английский

Процитировано

45

Development of an Agrobacterium-mediated CRISPR/Cas9 system in pea (Pisum sativum L.) DOI Creative Commons
Li Guan, Rong Liu,

Rongfang Xu

и другие.

The Crop Journal, Год журнала: 2022, Номер 11(1), С. 132 - 139

Опубликована: Май 24, 2022

Pea (Pisum sativum L.) is an annual cool-season legume crop. Owing to its role in sustainable agriculture as both a rotation and cash crop, global market expanding increased production urgently needed. For technical regulatory reasons, neither conventional nor transgenic breeding techniques can keep pace with the demand for production. In answer this challenge, CRISPR/Cas9 genome editing technology has been gaining traction plant biology crop recent years. However, there are currently no reports of successful application pea. We developed transient transformation system hairy roots, mediated by Agrobacterium rhizogenes strain K599, validate efficiency system. Further optimization resulted efficient vector, PsU6.3-tRNA-PsPDS3-en35S-PsCas9. used optimized edit pea phytoene desaturase (PsPDS) gene, causing albinism, Agrobacterium-mediated genetic transformation. This first report generation gene-edited plants route.

Язык: Английский

Процитировано

45

Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation DOI Creative Commons
Hang Su, Yuanchun Wang, Jin Xu

и другие.

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Июль 4, 2023

Citrus canker caused by Xanthomonas citri subsp. (Xcc) is a destructive citrus disease worldwide. Generating disease-resistant cultivars the most effective, environmentally friendly and economic approach for control. However, traditional breeding lengthy laborious. Here, we develop transgene-free canker-resistant sinensis lines in T0 generation within 10 months through transformation of embryogenic protoplasts with Cas12a/crRNA ribonucleoprotein to edit susceptibility gene CsLOB1. Among 39 regenerated lines, 38 are biallelic/homozygous mutants, demonstrating 97.4% mutation rate. No off-target mutations detected edited lines. Canker resistance cslob1-edited results from both abolishing symptoms inhibiting Xcc growth. The C. have received regulatory approval USDA APHIS exempted EPA regulation. This study provides sustainable efficient control solution presents an genome-editing strategy other crops.

Язык: Английский

Процитировано

40