Microbial Pathogenesis, Год журнала: 2025, Номер 204, С. 107595 - 107595
Опубликована: Апрель 15, 2025
Язык: Английский
Molecular Plant Pathology, Год журнала: 2024, Номер 25(2)
Опубликована: Фев. 1, 2024
Sclerotinia sclerotiorum is a cosmopolitan and typical necrotrophic phytopathogenic fungus that infects hundreds of plant species. Because no cultivars highly resistant to S. are available, managing disease caused by still challenging. However, recent studies have demonstrated has beneficial effect can live mutualistically as an endophyte in graminaceous plants, protecting the plants against major fungal diseases. An in-depth understanding schizotrophic lifestyle during interactions with under different environmental conditions will provide new strategies for controlling disease. In this review, we summarize pathogenesis mechanisms its attack host destructive pathogen discuss endophytic fungus.
Язык: Английский
Процитировано
15
Scientific Reports, Год журнала: 2024, Номер 14(1)
Опубликована: Март 11, 2024
Abstract The present study reports the green synthesis of silver nanoparticles (AgNPs) in powder form using leaf extract Azadirachta indica . AgNPs was confirmed by UV–vis spectroscopy, FTIR, XRD, FESEM, and EDX. synthesized were a powdered state dispersed completely 5% polyethylene glycol (PEG) demonstrated prolonged shelf life enhanced bioavailability over year without any aggregation. resulting nanoformulation complete inhibition against Sclerotinia sclerotiorum Colletotrichum falcatum 68% to 80% gloeosporioides Rhizoctonia solani respectively, at 2000 ppm. EC 50 values determined through statistical analysis 66.42, 157.7, 19.06, 33.30 ppm for S. , C. R. respectively. also established significant cytotoxicity, with 74.96% rate human glioblastoma cell line U87MG 250 IC value cancerous lines be 56.87 analysis. proposed may used as next-generation fungicide crop improvement find application anticancer investigations. To best our knowledge, this is first report demonstrating economically phytopathogen
Язык: Английский
Процитировано
15
Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 16, 2025
The development of rapeseed with high resistance against the pathogen Sclerotinia sclerotiorum is impeded by lack effective resources within host species. Unraveling molecular basis nonhost (NHR) holds substantial value for improvement in crops. In present study, small RNA sequencing and transcriptome were carried out between rice (a species S. sclerotiorum) during infection, revealing involvement miRNAs on translation-related processes both pathogen. Specifically, rice-specific potential capability cross-kingdom RNAi explored, which Os-miR169y was selected as a representative case to elucidate its role sclerotiorum. silence decreased level sclerotiorum, heterologous expression Arabidopsis significantly enhanced resistance. dual-luciferase reporter assay indicates that targets 60S ribosomal protein L19 (SsRPL19). Overexpressing (OEss-miR169y) SsRPL19 (RNAiss-RPL19) impaired growth pathogenicity pathogen, while overexpressing exhibited contrast effect. Yeast-two-hybridization revealed an interlinking multiple large subunits, indicating important translation. Proteome detected amount proteins transformants OEss-miR169y RNAiss-RPL19 significant suppression key metabolic pathways such carbon nitrogen metabolisms. Collectively, this study suggests can secrete specific suppress genes essential Os-miR169y, suppresses thus impairs synthesis This sheds light intrinsic mechanisms NHR further demonstrates using nonhost-specific "pathogen-attacking" improving
Язык: Английский
Процитировано
2
Frontiers in Plant Science, Год журнала: 2023, Номер 14
Опубликована: Июнь 14, 2023
In the search for new crop protection microbial biocontrol agents, isolates from genus
Язык: Английский
Процитировано
22
Phytopathology, Год журнала: 2024, Номер 114(6), С. 1253 - 1262
Опубликована: Янв. 3, 2024
, the causal agent of white mold infection, is a cosmopolitan fungal pathogen that causes major yield losses in many economically important crops. Spray-induced gene silencing has recently been shown to be promising alternative method for controlling plant diseases. Based on our prior research, we focused developing spray-induced approach control by
Язык: Английский
Процитировано
8
Journal of Nanobiotechnology, Год журнала: 2023, Номер 21(1)
Опубликована: Май 25, 2023
The biogenic synthesis of metallic nanoparticles is a green alternative that reduces the toxicity this nanomaterials and may enable synergy between core biomolecules employed in process enhancing biological activity. aim study was to synthesize titanium using filtrate fungus Trichoderma harzianum as stabilizing agent, obtain potential activity against phytopathogens mainly stimulate growth T. harzianum, its efficacy for control.The successful reproductive structures remained suspension, showing faster larger mycelial compared commercial filtrate. with residual showed inhibitory Sclerotinia sclerotiorum formation new resistant structures. A great chitinolytic observed comparison harzianum. In regard evaluation, an absence cytotoxicity protective effect through MTT Trypan blue assay. No genotoxicity on V79-4 3T3 cell lines while HaCat higher sensitivity. Microorganisms agricultural importance were not affected by exposure nanoparticles, however decrease number nitrogen cycling bacteria observed. phytotoxicity, did cause morphological biochemical changes soybean plants.The production essential factor stimulating or maintaining are important control, be strategy biocontrol organisms promote more sustainable agriculture.
Язык: Английский
Процитировано
16
Biological Control, Год журнала: 2023, Номер 186, С. 105346 - 105346
Опубликована: Сен. 9, 2023
Biological control using antagonistic microorganisms as biological agents (BCAs) has been considered a potential environmentally-friendly alternative or supplement for the of soil-borne plant pathogens. This may be part an integrated disease management system, thus contributing to reduction in use chemical products and protection environment. review aims provide updated overview insight into underlying mechanisms BCAs directly indirectly suppress Sclerotinia sclerotiorum, economically devastating fungal pathogen broad range plants. Further, application organic amendments (OAs) demonstrated pathogens through introduction soil and/or enhancing activity existing microbiome which improves general health. Therefore, manipulation exploitation microbial communities, OAs, sustainable strategy pathogens, particularly S. sclerotiorum. In this review, we highlight gaps current understanding interaction BCAs, sclerotiorum suggest future directions research space, including improving our microbiomes soils OAs suppressive
Язык: Английский
Процитировано
16
Biological reviews/Biological reviews of the Cambridge Philosophical Society, Год журнала: 2024, Номер 99(3), С. 1075 - 1084
Опубликована: Янв. 29, 2024
ABSTRACT Phenology is a key adaptive trait of organisms, shaping biotic interactions in response to the environment. It has emerged as critical topic with implications for societal and economic concerns due effects climate change on species' phenological patterns. Fungi play essential roles ecosystems, plant pathogenic fungi have significant impacts global food security. However, phenology fungi, which form huge diverse clade received limited attention literature. This diversity may use common language comparisons integration data these taxonomic groups. Here, we delve into concept ‘phenology’ applied explore potential drivers their phenology, including environmental factors host plant. We present PhenoFun scale, scoring system suitable all fungus‐like pathogens. offers standardised tool scientists studying presence, absence, or predominance particular phase, speed phase succession, synchronism shift between plants, across wide range environments ecosystems. The application involves focusing interacting processes hosts, biological, physical, chemical environment, occurring during life cycle pathogen. goal deconstruct involved according pattern orchestrated by fungus's phenology. Such an approach will improve our understanding ecology evolution such help understand anticipate disease epidemics future evolution, make it possible optimise management models, encourage adoption cropping practices designed from this perspective.
Язык: Английский
Процитировано
6
Physiological and Molecular Plant Pathology, Год журнала: 2024, Номер 130, С. 102239 - 102239
Опубликована: Фев. 6, 2024
Язык: Английский
Процитировано
6
Journal of Taibah University for Science, Год журнала: 2024, Номер 18(1)
Опубликована: Март 27, 2024
Effective plant pathogen control presents an important challenge. Pesticide resistance and associated health environmental problems demonstrate the need for novel, safe active ingredients protection. Since both coumarins 1,2,4-triazoles show pesticidal activity, in this study, we evaluated antimicrobial activity of coumarin-1,2,4-triazole hybrids against pathogenic fungi (Fusarium oxysporum, Fusarium culmorum, Macrophomina phaseolina Sclerotinia sclerotiorum), bacterial pathogens (Pseudomonas syringae Rhodococcus fascians), beneficial bacteria (Bacillus mycoides Bradyrhizobium japonicum). Coumarin-1,2,4-triazoles inhibited growth S. sclerotiorum F. while no antibacterial effect on either or soil-beneficial was observed. A quantitative structure–activity relationship models antifungal activities developed using Dragon descriptors, can explain 79% 77% compounds inhibitory respectively. According to molecular docking, title are potential sterol 14α-demethylase inhibitors, with 7-((5-mercapto-4-(p-tolyl)-4H-1,2,4-triazol-3-yl)methoxy)-4-methyl-2H-chromen-2-one as promising candidate further research
Язык: Английский
Процитировано
6