Berry shrivel in grapevine: a review considering multiple approaches

Journal of Experimental Botany, Год журнала: 2024, Номер 75(8), С. 2196 - 2213

Опубликована: Янв. 4, 2024

Grapevine berry shrivel, a ripening disorder, causes significant economic losses in the worldwide wine and table grape industries. An early interruption leads to this resulting shriveling reduced sugar accumulation affecting yield fruit quality. Loss of sink strength associated with mesocarp cell death is an symptom disorder; however, potential internal or external triggers are yet be explored. No pathogens have been identified that might cause syndrome. Understanding underlying mechanisms contributing shrivel crucial for developing effective mitigation strategies finding solutions other disorders climacteric non-climacteric fruits. This review discusses alterations mechanism induced by focusing primarily on transport metabolism, wall modification death, changes phytohormone profile. The essential open questions highlighted analyzed, thus identifying critical knowledge gaps key challenges future research.

Язык: Английский

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Grape berry native yeast microbiota: advancing trends in the development of sustainable vineyard pathogen biocontrol strategies

OENO One, Год журнала: 2024, Номер 58(1)

Опубликована: Янв. 9, 2024

Grape berry is an ecological niche for a myriad of microbes, whose interactions with one another modulate fruit health and can play role in fermentation, imparting character distinctiveness to wines. With the growing concerns about awareness risks associated overuse chemical pesticides viticulture, microbial-based pest control increasingly encouraged as more sustainable environmentally friendly strategy. The use yeasts from grape berries promising alternative vineyard diseases their increasing acceptance rapidly changing our perception fungicides. In this paper, we provide overview latest methodologies characterising dynamics context disease management, discuss prospects effective biocontrol agents viticulture. Most research has focused on rots produced by Botrytis, Aspergillus, Colletotrichum Penicillium spp. using formulations that comprise single strains specific yeast genera, including Aureobasidium, Metschnikowia, Saccharomyces, Pichia, Candida Rhodotorula. However, challenging disparity between successful outcomes obtained vitro low applicability field major limitation large-scale implementation these strategies. Novel approaches maximising stability efficiency yeast-derived bioactives are discussed review.

Язык: Английский

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On the Way to the Technological Development of Newly Selected Non-Saccharomyces Yeasts Selected as Innovative Biocontrol Agents in Table Grapes

Microorganisms, Год журнала: 2024, Номер 12(2), С. 340 - 340

Опубликована: Фев. 6, 2024

Post-harvest decay of fresh table grapes causes considerable annual production losses. The main fungal agents both in pre- and post-harvest are B. cinerea, Penicillium spp., Aspergillus Alternaria Cladosporium spp. To date, the use agrochemicals SO2 methods to control grape molds postharvest, respectively. Significant improvements, however, have already been made apply innovative more environmentally sustainable strategies, such as Biological Control Agents (BCAs), which can reduce disease severity post-harvest. In this study, 31 new non-Saccharomyces yeast strains, isolated from berries native Apulian genotypes, were tested for their vivo effectiveness against grey mold grapes, resulting two St. bacillaris (‘N22_I1’ ‘S13_I3’), one S. diversa (‘N22_I3’), A. pullulans (‘OLB_9.1_VL’) H. uvarum (‘OLB_9.1_BR’) strains that marked efficient good BCAs. Their mechanisms action characterized through vitro assays, additional characteristics evaluated assess economic feasibility viability future technological employment. was by reducing working concentration, antagonistic effect on a wide range pathogens, ability survive formulations with long shelf life, safety human health.

Язык: Английский

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Novel loci associated with resistance to downy and powdery mildew in grapevine

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Март 22, 2024

Among the main challenges in current viticulture, there is increasing demand for sustainability protection from fungal diseases, such as downy mildew (DM) and powdery (PM). Breeding disease-resistant grapevine varieties a key strategy better managing fungicide inputs. This study explores diversity of germplasm (cultivated wild) Caucasus neighboring areas to identify genotypes resistant DM PM, based on 13 Simple Sequence Repeat (SSR) loci phenotypical (artificial pathogen inoculation) analysis, associated with PM resistance, via Genome-Wide Association Analysis (GWAS) Single Nucleotide Polymorphism (SNP) profiles. SSR analysis revealed alleles 16 out 88 genotypes. Phenotypic data identified seven 31 GWAS two new located chromosome 15 (designated Rpv36 Rpv37 ), 6 17 Ren14 Ren15 ). The four novel genomic regions rich genes related biotic stress response, involved recognition, signal transduction resistance response. highlights potential candidate providing valuable insights breeding programs varieties. To optimize their utilization, further functional characterization studies are recommended.

Язык: Английский

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Secondary metabolites in grapevine: crosstalk of transcriptional, metabolic and hormonal signals controlling stress defence responses in berries and vegetative organs

Frontiers in Plant Science, Год журнала: 2023, Номер 14

Опубликована: Июнь 19, 2023

Abiotic stresses, such as temperature, heat waves, water limitation, solar radiation and the increase in atmospheric CO 2 concentration, significantly influence accumulation of secondary metabolites grapevine berries at different developmental stages, vegetative organs. Transcriptional reprogramming, miRNAs, epigenetic marks hormonal crosstalk regulate metabolism berries, mainly phenylpropanoids volatile organic compounds (VOCs). Currently, biological mechanisms that control plastic response cultivars to environmental stress or occur during berry ripening have been extensively studied many worlds viticultural areas, vines grown under various agronomic managements. A novel frontier study these is involvement miRNAs whose target transcripts encode enzymes flavonoid biosynthetic pathway. Some miRNA-mediated regulatory cascades, post-transcriptionally key MYB transcription factors, showing, for example, a role influencing anthocyanin UV-B light ripening. DNA methylation profiles partially affect transcriptome plasticity cultivars, contributing modulation qualitative traits. Numerous hormones (such abscisic jasmomic acids, strigolactones, gibberellins, auxins, cytokynins ethylene) are involved triggering vine abiotic biotic factors. Through specific signaling mediate antioxidants contribute quality intervene defense processes, highlighting stressors can be similar The expression genes responsible hormone biosynthesis largely modulated by conditions, thus resulting numeourous interactions between surrounding environment.

Язык: Английский

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Bunch microclimate influence amino acids and phenolic profiles of Pinot noir grape berries

Frontiers in Plant Science, Год журнала: 2023, Номер 14

Опубликована: Июнь 7, 2023

The increase of temperature due to climate change at different phenological stages grapevine has already been demonstrated affect accumulation primary and secondary metabolites in grape berries. This a significant implication for Pinot noir especially New Zealand context as these compounds can have direct indirect effects on wine quality.This study investigates how varying bunch microclimate through changes applied veraison stage affect: fresh weight, total soluble solids, the anthocyanins, phenolics amino acids was studied over two growing seasons (2018/19 2019/20) with vines being grown temperatures controlled environment (CE) chambers. were exposed 800 µmol/m2/s irradiance diurnal day (22°C or 30°C) night (15°C) temperatures. experimental set up enabled us determine metabolite harvest (both seasons) throughout berry development (second season).The results showed that weight not influenced by increase. solids (TSS) significantly increased 30°C, however, this expense (i.e., water loss). Anthocyanin content reduced higher first season but there no phenolic response treatments either season. concentrations second only. In addition, time course analysis season, mid-ripening ripening temperature. Significant qualitative acid composition specifically α-ketoglutarate family glutamine, arginine, proline) found between temperatures.This is provide detailed quantification individual which could aid develop adaptation strategies viticulture future.

Язык: Английский

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Biocontrol Potential of Endophytic Fungi Against Postharvest Grape Pathogens

Опубликована: Янв. 1, 2025

Язык: Английский

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Whey protein hydrolysates enhance grapevine resilience to abiotic and biotic stresses

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Май 9, 2025

Introduction The growing need for sustainable viticulture has increased interest in biostimulants that enhance plant resilience to abiotic and biotic stresses. This study evaluates the efficacy of whey-derived protein hydrolysates (PHs) improving Vitis vinifera cv. Cabernet Sauvignon tolerance combined heat drought stress reducing pathogen infections. Methods Potted grapevines were subjected 40°C without irrigation treated with either water or PHs. Physiological parameters as well key stress- photosynthesis-related genes expression monitored. antimicrobial effects PHs against Plasmopara viticola Botrytis cinerea also assessed. Results PHs-treated plants exhibited a faster recovery photosynthetic activity than control maintained normal sub-stomatal CO 2 concentrations under stress. treatment significantly upregulated stress-responsive ( HSFA2 , HSP101 ) mitigated stress-induced decline LHCA3 RbcS ). Moreover, enhanced grapevine tolerance, indicated by higher leaf potential values drought-responsive NCED1 TIP2;1 Additionally, demonstrated direct toxic effect on P. inhibiting zoospore germination sporulation discs, while B. infection berries when applied post-infection. Conclusion In tested conditions, whey serve effective biostimulants, enhancing providing protection pathogens. Although further validation vineyard conditions is needed, this dual benefit may propose alternative reduce chemical inputs viticulture, contributing more environmentally friendly agricultural practices.

Язык: Английский

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Grapevine virome and production of healthy plants by somatic embryogenesis

Microbial Biotechnology, Год журнала: 2022, Номер 15(5), С. 1357 - 1373

Опубликована: Фев. 19, 2022

Summary Grapevine ( Vitis spp.) is a widespread fruit tree hosting many viral entities that interact with the plant modifying its responses to environment. The production of virus‐free plants becoming increasingly crucial for use grapevine as model species in different studies. Using high‐throughput RNA sequencing, viromes seven mother grown germplasm collection vineyard were sequenced. In addition viruses and viroids already detected grapevine, we identified 13 putative new mycoviruses. spread among tissues collected vineyard, greenhouse vitro conditions suggested clear distinction between viruses/viroids mycoviruses can successfully be exploited their identification. Mycoviruses absent cultures, while particularly accumulated these plantlets. Somatic embryogenesis applied was effective elimination complete virome, including However, sanitization efficiencies pinot gris virus observed genotypes. absence plantlets, associated all somaclones, this regeneration technique also eradicate endophytic/epiphytic fungi, resulting gnotobiotic or pseudo‐gnotobiotic plants.

Язык: Английский

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Unravelling molecular mechanisms involved in resistance priming against downy mildew (Plasmopara viticola) in grapevine (Vitis vinifera L.)

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Сен. 6, 2023

Abstract Downy mildew (DM; Plasmopara viticola ) is amongst the most severe fungal diseases in viticulture and reason for majority of fungicide applications. To reduce synthetic copper-based fungicides, there an urgent need natural alternatives, which are being increasingly tested by industry research community. However, their mode action remains unclear. Therefore, our study aimed to investigate transcriptomic changes induced oregano essential oil vapour (OEOV) DM-infected grapevines. OEOV was applied at different time points before after DM infection differentiate between a priming effect direct effect. Both pre-DM treatment with post-infection resulted significant reduction sporulation. RNA-seq, followed differential gene expression weighted co-expression network analysis, identified co-expressed modules associated secondary metabolism, pathogen recognition response. Surprisingly, molecular mechanisms underlying efficiency against appear be independent stilbene synthesis, instead involve genes from putative signalling pathway that has yet characterized. This enhances understanding regulation innate plant immunity provides new insights into alternative antifungal agents.

Язык: Английский

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