Agronomy,
Год журнала:
2025,
Номер
15(5), С. 1071 - 1071
Опубликована: Апрель 28, 2025
Pink-flowered
Oriental
lily
cultivars
exhibit
significant
color
fading
under
high
temperatures,
but
the
underlying
regulatory
mechanisms
remain
unclear.
We
subjected
‘Souvenir’
plants
to
temperature
treatments
(20
°C
and
35
°C)
performed
transcriptome
sequencing
weighted
gene
co-expression
network
analysis
(WGCNA).
The
(35
significantly
reduced
anthocyanin
content
in
tepals.
identified
8354
differentially
expressed
genes,
with
GO
KEGG
analyses
revealing
a
dynamic
transition
from
early
stress
responses
metabolic
adaptation.
WGCNA
revealed
module
strongly
correlated
content,
which
we
constructed
using
known
anthocyanin-related
including
key
transcription
factor
LhMYB12
structural
genes
involved
biosynthetic
pathway
(LhANS,
LhDFR,
LhUGT78,
LhF3′H).
Through
this
comprehensive
analysis,
successfully
screened
LhERF109
as
promising
candidate.
transient
overexpression
of
was
found
enhance
accumulation
upregulate
LhMYB12,
while
silencing
expression
produced
opposite
effects.
These
findings
identify
positive
regulator
biosynthesis
providing
new
targets
for
breeding
heat-tolerant
lilies
stable
flower
coloration.
Biology,
Год журнала:
2025,
Номер
14(3), С. 258 - 258
Опубликована: Март 4, 2025
In
plants,
the
R2R3-MYB
transcription
factors
are
one
of
largest
MYB
gene
families.
These
very
important
for
regulating
plant
growth
and
development.
RcMYB114,
RcbHLH,
RcWD40
promote
anthocyanin
accumulation
by
forming
MBW
(MYB-bHLH-WD40)
complex
determine
rose
flower’s
color.
RcMYB114
genomic
sequences
differ
between
red
petal
white
varieties.
Two
non-synonymous
substitutions
were
found
in
open
reading
frame.
It
leads
to
a
change
amino
acids.
Here,
content
showed
that
there
was
no
petals,
while
petals
increased
firstly
at
stage
2,
decreased
slightly
4,
then
again
5.
The
spatiotemporal
expression
pattern
analysis
not
expressed
all
tissues
different
flower
development
stages.
varieties,
highly
followed
styles,
stems,
young
leaves,
1
However,
has
highest
level
blooming
stage.
sequence
contains
9
SNPs
coding
region,
7
which
synonymous
had
effect
on
translation
product
2
resulted
acid
alteration
positions
116
195,
respectively.
named
RcMYB114a,
RcMYB114b.
RcMYB114c
mutated
into
leucine
via
artificial
mutation;
it
valine
position
Glycine
Arginine
195
RcMYB114a
RcMYB114d.
RcMYB114b
double
mutation
RcMYB114a.
results
yeast
two-hybrid
experiments
its
missense
mutations
RcMYB114b,
RcMYB114c,
RcMYB114d
could
both
interact
with
RcbHLH
form
MYB-bHLH-WD40
complex.
A
transient
transformation
experiment
tobacco
confirmed
significantly
high
related
structural
genes
tobacco,
together
gene,
led
anthocyanins
produced
color
leaves.
interacted
regulated
anthocyanins.
two
do
affect
function
itself,
but
accumulated
different.
This
study
should
provide
clues
references
further
research
molecular
mechanism
underlying
determination
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(8), С. 3457 - 3457
Опубликована: Апрель 8, 2025
The
seed-coat
color
and
seed
size
have
an
impact
on
both
the
evolutionary
fitness
grain
yield
of
crops.
Soybean
is
a
major
oil
crop,
exhibit
natural
diversity
among
different
soybean
varieties.
Here,
we
found
R2R3-MYB
transcription
factor
GmMYB62,
which
shows
significant
increase
in
expression
as
changes
from
yellow
to
black
GmMYB62
was
specifically
highly
expressed
reproductive
organs,
especially
floral
organs
soybeans.
encodes
nuclear
protein
that
contains
two
MYB
domains.
In
phylogenetic
analysis,
relatively
conserved
after
divergence
monocots
dicots,
it
also
grouped
with
transcriptional
repressors
MYBs
anthocyanin
synthesis.
overexpressed
Arabidopsis
seeds
displayed
pale-brown
coat
overexpression
lines,
contrast
dark-brown
observed
wild-type
Col-0.
content
lines
dramatically
reduced
when
compared
Additionally,
showed
shorter
lengths,
larger
widths,
lower
thousand-seed
weights
than
those
Furthermore,
genes
related
synthesis
regulation
were
investigated,
eight
involved
pathway,
like
chalcone
synthase
(CHS),
isomerase
(CHI),
flavanone
3-hydroxylase
(F3H),
anthocyanidin
(ANS)
severely
inhibited
addition,
ARGOS-LIKE
(ARL),
B-Type
Cyclin
1
(CYCB1),
enhancer
DA1-1
(EOD3),
govern
cell
expansion
proliferation,
Overall,
this
study
sheds
new
light
control
by
provides
potentially
valuable
targets
for
improving
crop
quality.
Agronomy,
Год журнала:
2025,
Номер
15(5), С. 1071 - 1071
Опубликована: Апрель 28, 2025
Pink-flowered
Oriental
lily
cultivars
exhibit
significant
color
fading
under
high
temperatures,
but
the
underlying
regulatory
mechanisms
remain
unclear.
We
subjected
‘Souvenir’
plants
to
temperature
treatments
(20
°C
and
35
°C)
performed
transcriptome
sequencing
weighted
gene
co-expression
network
analysis
(WGCNA).
The
(35
significantly
reduced
anthocyanin
content
in
tepals.
identified
8354
differentially
expressed
genes,
with
GO
KEGG
analyses
revealing
a
dynamic
transition
from
early
stress
responses
metabolic
adaptation.
WGCNA
revealed
module
strongly
correlated
content,
which
we
constructed
using
known
anthocyanin-related
including
key
transcription
factor
LhMYB12
structural
genes
involved
biosynthetic
pathway
(LhANS,
LhDFR,
LhUGT78,
LhF3′H).
Through
this
comprehensive
analysis,
successfully
screened
LhERF109
as
promising
candidate.
transient
overexpression
of
was
found
enhance
accumulation
upregulate
LhMYB12,
while
silencing
expression
produced
opposite
effects.
These
findings
identify
positive
regulator
biosynthesis
providing
new
targets
for
breeding
heat-tolerant
lilies
stable
flower
coloration.
