Horticulture Research,
Год журнала:
2024,
Номер
11(10)
Опубликована: Июль 30, 2024
Lily
(Lilium
spp.),
a
horticultural
crop
serving
both
ornamental
and
edible
functions,
derives
its
coloration
primarily
from
anthocyanins.
However,
limited
studies
have
been
conducted
on
the
accumulation
of
anthocyanins
within
lilies.
In
this
study,
we
cloned
light-induced
transcription
factor
named
as
LvBBX24
in
Through
genetic
biochemical
analysis,
determined
that
could
upregulate
LvMYB5
facilitate
anthocyanin
synthesis.
Moreover,
identified
darkness
promoted
degradation
protein.
screening
yeast
library,
LvbZIP44
acts
interacting
partner.
Genetic
testing
confirmed
also
plays
role
promoting
lily
This
indicates
potential
synergistic
regulatory
effect
between
LvbZIP44.
Our
study
cooperate
to
regulate
petals.
These
findings
provide
compelling
evidence
supporting
idea
may
form
looped
helix
surrounding
promoter
region
biosynthesis.
Horticulturae,
Год журнала:
2024,
Номер
10(9), С. 926 - 926
Опубликована: Авг. 30, 2024
Anthocyanins,
constituents
of
flavonoid
compounds
prevalent
in
plants,
possess
significant
value
both
plant
development
and
human
nutrition.
The
regulation
anthocyanin
biosynthesis
primarily
involves
the
orchestration
MYB,
bHLH,
WD40
transcription
factors.
Consequently,
bHLH
family
assumes
a
pivotal
role
modulating
developmental
processes.
In
present
investigation,
factor,
denoted
as
LvbHLH13,
was
identified
positive
regulator
pigmentation
lily
petals.
LvbHLH13
is
classified
within
IIId
subgroup
Arabidopsis
proteins.
Functional
analyses
involving
transient
expression
gene
silencing
revealed
its
capacity
to
enhance
diminish
accumulation,
respectively,
by
LvMYB5
expression,
thereby
influencing
downstream
structural
expression.
overexpression
resulted
an
increase
genes
related
synthesis,
whereas
correspondingly
decreased
Yeast
one-hybrid
EMSA
assays
demonstrated
interaction
between
promoter,
leading
activation
biosynthesis.
A
further
luciferase
(LUC)
analysis
corroborated
stimulatory
effect
on
promoter
sequence.
assumed
crucial
lily-petal
pigmentation.
yeast
two-hybrid
that
diverged
from
typical
factor
behavior
it
did
not
form
complex
with
MYB
regulate
This
discrepancy
could
be
attributed
deletion
N-terminal
conserved
sequence
LvbHLH13.
study
provides
new
candidate
bHLH-MYB
partner
explore
regulatory
network
research
opportunities
for
breeding
lilies
various
contents.
These
findings
lay
theoretical
foundation
subsequent
investigations
into
flower
coloring
mechanisms.
Plants,
Год журнала:
2025,
Номер
14(11), С. 1610 - 1610
Опубликована: Май 25, 2025
Severe
physiological
fruit
abscission
significantly
limits
yield
potential
in
macadamia.
Polygalacturonase
(PG),
a
key
hydrolytic
enzyme
pectin
degradation,
plays
critical
role
abscission.
However,
the
macadamia
genome,
PG
gene
family
and
members
involved
remain
poorly
understood.
In
this
study,
56
members,
which
were
unevenly
distributed
across
13
of
14
chromosomes,
identified
genome.
Phylogenetic
analysis
clustered
these
genes
into
seven
clades,
with
most
found
clades
D
E.
The
MiPGs
contained
3–11
exons
2–10
introns,
except
for
those
E
G,
conserved
domains
I–IV
predicted
to
be
localized
exclusively
cell
membrane.
MiPG
promoter
revealed
numerous
light-,
phytohormone-,
stress-responsive
cis-elements.
Expression
profiling
during
development
showed
that
twelve
either
undetectable
or
expressed
at
low
levels
zone,
whereas
eight
highly
expressed.
MiPG9,
MiPG37,
MiPG53
upregulated
induced
by
combination
girdling
defoliation
ethephon
treatments.
Moreover,
transient
MiPG37
overexpression
lily
petals
promoted
premature
abscission,
suggesting
pivotal
These
findings
advance
functional
characterization
highlight
subset
candidate
further
genetic
manipulation
improve
retention.
Horticulture Research,
Год журнала:
2024,
Номер
11(10)
Опубликована: Июль 30, 2024
Lily
(Lilium
spp.),
a
horticultural
crop
serving
both
ornamental
and
edible
functions,
derives
its
coloration
primarily
from
anthocyanins.
However,
limited
studies
have
been
conducted
on
the
accumulation
of
anthocyanins
within
lilies.
In
this
study,
we
cloned
light-induced
transcription
factor
named
as
LvBBX24
in
Through
genetic
biochemical
analysis,
determined
that
could
upregulate
LvMYB5
facilitate
anthocyanin
synthesis.
Moreover,
identified
darkness
promoted
degradation
protein.
screening
yeast
library,
LvbZIP44
acts
interacting
partner.
Genetic
testing
confirmed
also
plays
role
promoting
lily
This
indicates
potential
synergistic
regulatory
effect
between
LvbZIP44.
Our
study
cooperate
to
regulate
petals.
These
findings
provide
compelling
evidence
supporting
idea
may
form
looped
helix
surrounding
promoter
region
biosynthesis.
