Genome Editing for Sustainable Crop Improvement and Mitigation of Biotic and Abiotic Stresses

Plants, Год журнала: 2022, Номер 11(19), С. 2625 - 2625

Опубликована: Окт. 6, 2022

Climate change poses a serious threat to global agricultural activity and food production. Plant genome editing technologies have been widely used develop crop varieties with superior qualities or can tolerate adverse environmental conditions. Unlike conventional breeding techniques (e.g., selective mutation breeding), modern tools offer more targeted specific alterations of the plant could significantly speed up progress developing crops desired traits, such as higher yield and/or stronger resilience changing environment. In this review, we discuss current development future applications in mitigating impacts biotic abiotic stresses on agriculture. We focus specifically CRISPR/Cas system, which has center attention last few years revolutionary genome-editing tool various species. also conducted bibliographic analysis CRISPR-related papers published from 2012 2021 (10 years) identify trends potential CRISPR/Cas-related research. addition, review article outlines shortcomings challenges employing agriculture notes prospective. believe combining innovative would be key optimizing improvement beyond limitations traditional practices.

Язык: Английский

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A short review on sugarcane: its domestication, molecular manipulations and future perspectives

Genetic Resources and Crop Evolution, Год журнала: 2022, Номер 69(8), С. 2623 - 2643

Опубликована: Сен. 16, 2022

Язык: Английский

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CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane

Frontiers in Plant Science, Год журнала: 2024, Номер 14

Опубликована: Янв. 8, 2024

Gene editing technologies have opened up the possibility of manipulating genome any organism in a predicted way. CRISPR technology is most used tool and, agriculture, it has allowed expansion possibilities plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA prime and nucleic acid probing detection. This mostly depends on

Язык: Английский

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Leveraging the sugarcane CRISPR/Cas9 technique for genetic improvement of non-cultivated grasses

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Март 27, 2024

Under changing climatic scenarios, grassland conservation and development have become imperative to impart functional sustainability their ecosystem services. These goals could be effectively efficiently achieved with targeted genetic improvement of native grass species. To the best our literature search, very scant research findings are available pertaining gene editing non-cultivated species (switch grass, wild sugarcane, Prairie cordgrass, Bermuda Chinese silver etc.) prevalent in natural semi-natural grasslands. Thus, explore this novel aspect, study purposes that techniques employed for cultivated grasses especially sugarcane might used as well. Our hypothesis behind suggesting a model crop is intricacy owing polyploidy aneuploidy compared other (rice, wheat, barley, maize, etc.). Another reason genome protocols ( x = 10–13) been developed optimized, taking into consideration high level redundancy. per knowledge, review first objectively evaluates concept functioning CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 technique regarding versatility, target specificity, efficiency, design simplicity, multiplexing capacity order perspectives against biotic abiotic stresses. Additionally, pronounced challenges confronting resulted different variants (Cas9, Cas12a, Cas12b, SpRY) tool, whose technicalities also critically assessed. Moreover, limitations emerge during highlighted.

Язык: Английский

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Genetic Engineering for Enhancing Sugarcane Tolerance to Biotic and Abiotic Stresses

Plants, Год журнала: 2024, Номер 13(13), С. 1739 - 1739

Опубликована: Июнь 24, 2024

Sugarcane, a vital cash crop, contributes significantly to the world's sugar supply and raw materials for biofuel production, playing significant role in global industry. However, sustainable productivity is severely hampered by biotic abiotic stressors. Genetic engineering has been used transfer useful genes into sugarcane plants improve desirable traits emerged as basic applied research method maintain growth under different adverse environmental conditions. use of transgenic approaches remains contentious requires rigorous experimental methods address biosafety challenges. Clustered regularly interspaced short palindromic repeat (CRISPR) mediated genome editing technology growing rapidly may revolutionize production. This review aims explore innovative genetic techniques their successful application developing cultivars with enhanced resistance stresses produce superior cultivars.

Язык: Английский

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From Transgenesis to Genome Editing in Crop Improvement: Applications, Marketing, and Legal Issues

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(8), С. 7122 - 7122

Опубликована: Апрель 12, 2023

The biotechnological approaches of transgenesis and the more recent eco-friendly new breeding techniques (NBTs), in particular, genome editing, offer useful strategies for genetic improvement crops, therefore, recently, they have been receiving increasingly attention. number traits improved through editing technologies is growing, ranging from resistance to herbicides insects capable coping with human population growth climate change, such as nutritional quality or climatic stress diseases. Research on both has reached an advanced stage development and, many biotech phenotypic evaluations open field are already underway. In addition, approvals regarding main crops granted. Over time, there increase areas cultivated that approaches, but their use various countries limited by legislative restrictions according different regulations applied which affect cultivation, marketing, animal nutrition. absence specific legislation, on-going public debate favorable unfavorable positions. This review offers updated in-depth discussion these issues.

Язык: Английский

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Sugarcane productivity and sugar yield improvement: Selecting variety, nitrogen fertilizer rate, and bioregulator as a first-line treatment

Heliyon, Год журнала: 2023, Номер 9(4), С. e15520 - e15520

Опубликована: Апрель 1, 2023

The improvement of sugarcane productivity depends on the crop varieties, growth environments, and management practices. In particular, selection most productive variety use an optimal fertilizer rate plant bioregulator are critical for increasing sugar yield. This study aimed to determine high-performing variety, nitrogen rate, potential improved production, juice quality, Two varieties (D42/58 NCo-334), three (N) rates (0, 100, 150 kg/ha), two bioregulators (Agrostemin Crops®) were used study. revealed that had a significant effect all growth, yield, quality parameters. Plant height was significantly influenced by bioregulators, while stalk population two- three-way interactions N rates. Cane weight affected whereas cane yield fertilizer, their interaction effects. Sugar similarly bioregulator, interaction. main factors, as well interactions, considerable influence Sugarcane D42/58 outperformed NCo-334 in terms sprouting, number tillers, height, millable canes, sucrose percentage 7.49%, 9.50%, 12.80%, 10.50%, 9.10%, respectively. with (at 100 kg/ha) and/or Agrostemin also led higher performance (107126), (153.34 tons/ha), Pol % (15.81%), (10.25 tons/ha). Most parameters positively correlated; hence, appropriate rates, could boost Overall, combination kg N/ha maximize To confirm current findings, however, more research needs be conducted across different agroecologies seasons.

Язык: Английский

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Transgene-Free Genome Editing for Biotic and Abiotic Stress Resistance in Sugarcane: Prospects and Challenges

Agronomy, Год журнала: 2023, Номер 13(4), С. 1000 - 1000

Опубликована: Март 29, 2023

Sugarcane (Saccharum spp.) is one of the most valuable food and industrial crops. Its production constrained due to major biotic (fungi, bacteria, viruses insect pests) abiotic (drought, salt, cold/heat, water logging heavy metals) stresses. The ever-increasing demand for sugar biofuel rise new pest disease variants call use innovative technologies speed up sugarcane genetic improvement process. Developing cultivars through conventional breeding techniques requires much time resources. advent CRISPR/Cas genome editing technology enables creation with improved resistance/tolerance various presence cassette inside genome-edited plants hinders commercial exploitation regulatory issues. However, this limitation can be overcome by using transgene-free techniques. Transgene-free approaches, such as delivery RNPs biolistics or protoplast fusion, virus-induced (VIGE), transient expression reagents Agrobacterium-mediated transformation other are discussed. A well-established PCR-based assay advanced screening systems visual marker system Transgene killer CRISPR (TKC) rapidly identify edits. These advancements in climate-smart that combat stresses produce good yields under ever-changing conditions.

Язык: Английский

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Biotechnological Advancements Toward Sugarcane Crop Improvement

Опубликована: Янв. 1, 2024

Sugarcane, is a vital tropical C4 plant known for its sucrose storage capacity, plays crucial role in global sugar and ethanol production. Despite multifaceted applications, the sugarcane industry faces numerous challenges, which impact final harvesting yield quality. Traditional breeding methods prove inefficient due to sugarcane's complexity extended cycles. Modern biotechnological approaches, particularly genetic engineering molecular markers, offer unprecedented opportunities. These techniques enable precise modifications, facilitating development of superior varieties. Molecular markers genomic tools aid identifying high-yield, stress-tolerant cultivars, enhancing selection processes. Additionally, advancements bioinformatics, genomics, proteomics, transcripteomic shed light on intricate gene-protein-metabolite relationships, understanding stress responses. This chapter explores these innovations, showcasing their potential address existing challenges ensure sustainable future production, thereby promoting food security sustainability.

Язык: Английский

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Sensitivity-enhanced self-powered biosensing platform for detection of sugarcane smut using Mn-doped ZIF-67, RCA-DNA nano-grid array and capacitor

Biosensors and Bioelectronics, Год журнала: 2025, Номер 273, С. 117182 - 117182

Опубликована: Янв. 18, 2025

Язык: Английский

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