Leveraging the sugarcane CRISPR/Cas9 technique for genetic improvement of non-cultivated grasses

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Март 27, 2024

Under changing climatic scenarios, grassland conservation and development have become imperative to impart functional sustainability their ecosystem services. These goals could be effectively efficiently achieved with targeted genetic improvement of native grass species. To the best our literature search, very scant research findings are available pertaining gene editing non-cultivated species (switch grass, wild sugarcane, Prairie cordgrass, Bermuda Chinese silver etc.) prevalent in natural semi-natural grasslands. Thus, explore this novel aspect, study purposes that techniques employed for cultivated grasses especially sugarcane might used as well. Our hypothesis behind suggesting a model crop is intricacy owing polyploidy aneuploidy compared other (rice, wheat, barley, maize, etc.). Another reason genome protocols ( x = 10–13) been developed optimized, taking into consideration high level redundancy. per knowledge, review first objectively evaluates concept functioning CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 technique regarding versatility, target specificity, efficiency, design simplicity, multiplexing capacity order perspectives against biotic abiotic stresses. Additionally, pronounced challenges confronting resulted different variants (Cas9, Cas12a, Cas12b, SpRY) tool, whose technicalities also critically assessed. Moreover, limitations emerge during highlighted.

Язык: Английский

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Standardization of Protocol for Maximizing Isolation Efficiency and Purity of Protoplasts and Lytic Vacuoles from Sugarcane Stalk

Sugar Tech, Год журнала: 2025, Номер unknown

Опубликована: Фев. 19, 2025

Язык: Английский

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Strategies for delivery of CRISPR/Cas-mediated genome editing to obtain edited plants directly without transgene integration

Frontiers in Genome Editing, Год журнала: 2023, Номер 5

Опубликована: Июль 20, 2023

Increased understanding of plant genetics and the development powerful easier-to-use gene editing tools over past century have revolutionized humankind's ability to deliver precise genotypes in crops. Plant transformation techniques are well developed for making transgenic varieties certain crops model organisms, yet reagent delivery regeneration remain key bottlenecks applying technology most Typical protocols produce transgenic, genetically modified (GM) rely on transgenes, chemical selection, tissue culture. make edited (GE) also use even though these may be undesirable final crop product. In some crops, transgenes routinely segregated away during meiosis by performing crosses, thus only a minor concern. other particularly those propagated vegetatively, complex hybrids, or with long generation times, such crosses impractical impossible. This review highlights diverse strategies CRISPR/Cas reagents regenerable cells recover plants without unwanted integration transgenes. Some examples include delivering DNA-free as ribonucleoproteins mRNA, relying expression from non-integrated DNA, using novel mechanisms viruses nanoparticles, unconventional selection methods avoid and/or avoiding culture altogether. These advancing rapidly already enabling scientists precision CRISPR tools.

Язык: Английский

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Isolation, Genome-Wide Identification and Expression Profiling of CYC/TB1 Like Transcription Factor from Saccharum Complex Under Water Deficit Stress Condition

Tropical Plant Biology, Год журнала: 2025, Номер 18(1)

Опубликована: Янв. 30, 2025

Язык: Английский

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RNAi and genome editing of sugarcane: Progress and prospects

The Plant Journal, Год журнала: 2025, Номер 121(5)

Опубликована: Март 1, 2025

Sugarcane, which provides 80% of global table sugar and 40% biofuel, presents unique breeding challenges due to its highly polyploid, heterozygous, frequently aneuploid genome. Significant progress has been made in developing genetic resources, including the recently completed reference genome sugarcane cultivar R570 pan-genomic resources from sorghum, a closely related diploid species. Biotechnological approaches RNA interference (RNAi), overexpression transgenes, gene editing technologies offer promising avenues for accelerating improvement. These methods have successfully targeted genes involved important traits such as sucrose accumulation, lignin biosynthesis, biomass oil stress response. One main transformation methods-biolistic transfer or Agrobacterium-mediated transformation-coupled with efficient tissue culture protocols, is typically used implementing these biotechnology approaches. Emerging show promise overcoming current limitations. The use morphogenic can help address genotype constraints improve efficiency. Tissue culture-free technologies, spray-induced silencing, virus-induced editing, potential functional genomics studies. Additionally, novel base prime orthogonal synthetic transcription factors, directed evolution present opportunities enhancing traits. advances collectively aim sugarcane's efficiency crop both biofuel production. This review aims discuss methodologies, focus on RNAi approaches, how be inform targets, future improvements applications.

Язык: Английский

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Integrative Metabolome Profiling and Docking Study of Sugar-Related Metabolites in Lytic Vacuoles of Mature Sugarcane Stem

Tropical Plant Biology, Год журнала: 2025, Номер 18(1)

Опубликована: Март 27, 2025

Язык: Английский

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Genome-Wide Identification, Characterization and Expression Analysis of Plant Nuclear Factor (NF-Y) Gene Family Transcription Factors in Saccharum spp.

Genes, Год журнала: 2023, Номер 14(6), С. 1147 - 1147

Опубликована: Май 25, 2023

Plant nuclear factor (NF-Y) is a transcriptional activating composed of three subfamilies: NF-YA, NF-YB, and NF-YC. These factors are reported to function as activators, suppressors, regulators under different developmental stress conditions in plants. However, there lack systematic research on the NF-Y gene subfamily sugarcane. In this study, 51 genes (ShNF-Y), 9 18 24 NF-YC genes, were identified sugarcane (Saccharum spp.). Chromosomal distribution analysis ShNF-Ys Saccharum hybrid located all 10 chromosomes. Multiple sequence alignment (MSA) ShNF-Y proteins revealed conservation core functional domains. Sixteen orthologous pairs between sorghum. Phylogenetic subunits sugarcane, sorghum, Arabidopsis showed that ShNF-YA equidistant while ShNF-YB ShNF-YC clustered distinctly, forming closely related divergent groups. Expression profiling drought treatment members involved tolerance its drought-tolerant wild relative, Erianthus arundinaceus. ShNF-YA5 ShNF-YB2 had significantly higher expression root leaf tissues both plant species. Similarly, ShNF-YC9 elevated E. arundinaceus hybrid. results provide valuable genetic resources for further crop improvement programs.

Язык: Английский

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Recent advances in genome editing strategies for balancing growth and defence in sugarcane (Saccharum officinarum)

Functional Plant Biology, Год журнала: 2024, Номер 51(5)

Опубликована: Май 1, 2024

Sugarcane (Saccharum officinarum ) has gained more attention worldwide in recent decades because of its importance as a bioenergy resource and producing table sugar. However, the production capabilities conventional varieties are being challenged by changing climates, which struggle to meet escalating demands growing global population. Genome editing emerged pivotal field that offers groundbreaking solutions agriculture beyond. It includes inserting, removing or replacing DNA an organism's genome. Various approaches employed enhance crop yields resilience harsh climates. These techniques include zinc finger nuclease (ZFN), transcription activator-like effector (TALEN) clustered regularly interspaced short palindromic repeats/associated protein (CRISPR/Cas). Among these, CRISPR/Cas is one most promising rapidly advancing fields. With help these techniques, several crops like rice (Oryza sativa ), tomato (Solanum lycopersicum maize (Zea mays barley (Hordeum vulgare sugarcane have been improved be resistant viral diseases. This review describes advances genome with particular focus on focuses advantages limitations while also considering regulatory ethical implications across different countries. insights into future prospects application agriculture.

Язык: Английский

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Isolation, Characterization and Genome-Wide Identification of Cys-2/His-2 Type Zinc Finger Nuclease, a Transcription Activator in Saccharum Complex Under Abiotic Stress Conditions

Sugar Tech, Год журнала: 2024, Номер unknown

Опубликована: Апрель 2, 2024

Язык: Английский

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Technological Innovation in Disease Detection and Management in Sugarcane Planting

Bioscience Methods, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

The objective of this study is to systematically examine recent technological innovations in disease detection and management within sugarcane cultivation. It seeks identify key advancements digital imaging, molecular diagnostics, genetic engineering that have significantly improved the detection, monitoring, control diseases, aiming enhance overall crop health productivity. This identifies several crucial technologies reshaped strategies highlights effectiveness machine learning algorithms remote sensing technology detecting diagnosing plant diseases at early stages. Developments diagnostics allowed for rapid precise pathogen identification. Additionally, has contributed creation disease-resistant varieties, thereby reducing dependency on chemical treatments. Integration these led surveillance management, resulting healthier crops increased yields. convergence learning, sensing, represents a transformative shift managing diseases. These not only ability detect manage more efficiently but also contribute sustainable agriculture practices by use improving resilience. Continued innovation integration hold promise further gains productivity sustainability agriculture.

Язык: Английский

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