Characterization of the broad-spectrum antibacterial activity of bacteriocin-like inhibitory substance-producing probiotics isolated from fermented foods

BMC Microbiology, Год журнала: 2024, Номер 24(1)

Опубликована: Март 11, 2024

Abstract Antimicrobial peptides, such as bacteriocin, produced by probiotics have become a promising novel class of therapeutic agents for treating infectious diseases. Selected lactic acid bacteria (LAB) isolated from fermented foods with probiotic potential were evaluated various tests, including exopolysaccharide production, antibiotic susceptibility, and bile tolerance, antibacterial activity, cell adhesion cytotoxicity to gastric lines. Six selected LAB strains maintained their high viability under gastrointestinal conditions, exopolysaccharides, showed no or less cytotoxicity, adhered successfully cells. Furthermore, three strains, Weissella confusa CYLB30, Lactiplantibacillus plantarum CYLB47, Limosilactobacillus fermentum CYLB55, demonstrated strong effect against drug-resistant Escherichia coli, Klebsiella pneumoniae , Pseudomonas aeruginosa Salmonella enterica serovar Choleraesuis, Enterococcus faecium Staphylococcus aureus. Whole genome sequencing was performed on these using the Nanopore platform; then, results that all did not harbor genes related toxins, superantigens, acquired antimicrobial resistance, in genome. The bacteriocin gene cluster found CYLB47 genome, but CYLB30 CYLB55 genomes. In SDS-PAGE, extract bacteriocin-like inhibitory substance (BLIS) yielded single band size than 10 kDa. These BLIS inhibited growth biofilm formation P. methicillin-resistant S. aureus (MRSA), causing membrane disruption inhibiting ability human skin HaCaT Moreover, rescued larvae after being infected MRSA infections. conclusion, may be alternative treatment multidrug-resistant

Язык: Английский

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Vulnerability, Resilience and Adaptation of Lagos Coastal Communities to Flooding

Earth Science Systems and Society, Год журнала: 2024, Номер 4

Опубликована: Июль 16, 2024

Lagos has been identified as one of the 50 cities most vulnerable to extreme sea levels. The state also ranked 30th among 136 port in terms population exposure flooding under a past climate scenario (2005) and 15th future (2070s). faces significant environmental strains result its geo-economic status. Some problems are wetland loss, pollution, pressure, restricted access drinkable water, flooding. All these factors have contributed instability ecosystems, but impact is particularly because surrounded by aquatic ecosystems low elevation topography increase susceptibility method adopted this review involved use PICO (Population, Intervention, Control, Outcomes) criteria synthesize research questions objectives. Thereafter, PRISMA (Preferred Reporting Items for Systematic Reviews Meta-Analysis) guidelines was employed study selection criteria, search strategies data extraction methods. A broad strategy involving databases (Google scholar, Science Direct), pertinent keywords filters used identify relevant articles minimize bias. After search, again select studies be considered review. selected text were downloaded essential scientific information extracted analyzed study. impacts numerous. Flooding key that prevented Africa’s growing urban from escaping poverty, it impedes achievement some SDGs. This many African lack resources infrastructures needed withstand weather conditions. Surviving flood-prone like daunting task flood affects livelihood, human health, can even cause death. Specifically, 2020 incidence Nigeria affected about 40% local government areas, 97% states, displacing over 120,000 persons killing 68 aside properties farmlands destroyed. Women children vulnerable. adoption advanced risk management could help containment state.

Язык: Английский

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Effect of processing on the microbiological, proximate, antinutritional and mineral profile of selected yellow cassava varieties and sorghum malt as potential raw materials for alcoholic beverage production

Beverage Plant Research, Год журнала: 2024, Номер 4(1), С. 0 - 0

Опубликована: Янв. 1, 2024

Sorghum and yellow cassava can be regarded as the top two raw materials in production of value-added food products due to their rich nutritional properties. Thus, this study was carried out effect these processing methods on microbiological, proximate, antinutritional mineral composition materials. The involved varieties one sorghum variety. Steeping, germination malting decreased aerobic plate count from values 2.40 × 10⁶ ± 0.22 cfu/g 1.53 0.32 2.51 10³ 0.05 1.21 0.02 respectively (p < 0.05). For both varieties, ranged between 5.37 10⁴ 0.26 9.40 0.17 fresh roots 2.49 10² 0.35 1.31 0.23 flours. Significant differences were observed for Malting increased crude protein 13.51 0.25% 14.84 0.42% Processing into flour reduced content 3.12 0.33% 3.36 0.15% 2.20 0.18% 2.44 0.13% respectively. phytate (from 42.37 0.89% 7.2 0.28%), oxalate (76.97 1.63% 20.54 0.92%) tannin (2.85 0.02% 0.4 0.02%) It concluded results that steeping, germination, malting, milling drying could used singly or combination a regime affect profile well reduce factors which utilized potential provision nutritious especially alcoholic beverage production.

Язык: Английский

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Evaluating the Role of Viable Cells, Heat-Killed Cells or Cell-Free Supernatants in Bacterial Biocontrol of Fungi: A Comparison Between Lactic Acid Bacteria and Pseudomonas

Microorganisms, Год журнала: 2025, Номер 13(1), С. 105 - 105

Опубликована: Янв. 7, 2025

This study investigated whether viable cells, dead cells or cell-free supernatants (CFS) were responsible for the biocontrol effect of strains from two important bacterial genera, Pseudomonas and Lactobacillus, known their antifungal properties against plant pathogens food spoilage microorganisms. Specifically, capability these to produce extracellular hydrolytic enzymes on specified media was assessed, along with effectiveness in inhibiting mycelial growth several phytopathogenic fungi (Fusarium oxysporum, Botrytis cinerea, Pythium ultimum Rhizoctonia solani) using dual culture plate assays. Results inhibition assays revealed that P. fluorescens PF05 L. plantarum LMG 23520 most effective suppressing fungal growth, especially F. oxysporum. Therefore, further experiments carried out investigate potential heat-killed (HKC) CFS germination oxysporum spores. The cell trial proved successful, whereas HKC isolates ineffective spore germination. Conversely, able prevent development up six days. PF05, instead, did not yield positive results. Additional studies are required evaluate inhibitory effects both strains.

Язык: Английский

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Leuconostoc mesenteroides strain MS4-derived bacteriocins: A potent antimicrobial arsenal for controlling Xylella fastidiosa infection

Microbiological Research, Год журнала: 2025, Номер 293, С. 128071 - 128071

Опубликована: Янв. 15, 2025

Язык: Английский

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Unlocking the potential of ecofriendly guardians for biological control of plant diseases, crop protection and production in sustainable agriculture

3 Biotech, Год журнала: 2025, Номер 15(4)

Опубликована: Март 9, 2025

Язык: Английский

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Importance of Lactic Acid Bacteria as an Emerging Group of Plant Growth-Promoting Rhizobacteria in Sustainable Agroecosystems

Applied Sciences, Год журнала: 2024, Номер 14(5), С. 1798 - 1798

Опубликована: Фев. 22, 2024

Increasing awareness of the problems caused by synthetic agrochemicals, such as chemical fertilizers, pesticides, and herbicides, makes it crucial to discover substitute approaches that can guarantee competitive plant production protect environment while maintaining natural balance in agroecosystems. One leading alternatives is utilizing rhizobacterial strains named growth-promoting rhizobacteria (PGPR). The utilization PGPR-based biofertilizers for advancement sustainability farming productions has received considerable critical attention all over world because their contribution not only improving growth but also inducing biotic abiotic stress tolerance. This review updates aforementioned eco-friendly strategy sustainable agroecosystems provides new insights into phytostimulation bioprotection ability lactic acid bacteria (LAB), an emerging taxon PGPR. In this regard, LAB synthesize metabolites, including organic acids, phenolic acids flavonoid derivatives, phytohormones, antimicrobial substrates, presented. use a bridge between PGPR environmentally friendly crop productivity, which lead systems reducing soil quality, minimizing environmental pollution. All beneficial aspects need be addressed future research plan systematic methodologies and/or combine along with other or inorganic inputs systems.

Язык: Английский

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Application of Bacillus Species in the Alleviation of Salinity-Stressed Agricultural Soil: An Overview

Microorganisms for sustainability, Год журнала: 2024, Номер unknown, С. 107 - 131

Опубликована: Янв. 1, 2024

Язык: Английский

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Metagenomic insights into the response of soil microbial communities to pathogenic Ralstonia solanacearum

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Фев. 16, 2024

Understanding the response of soil microbial communities to pathogenic Ralstonia solanacearum is crucial for preventing bacterial wilt outbreaks. In this study, we investigated physicochemical and community assess their impact on R.solanacearum through metagenomics. Our results revealed that certain archaeal taxa were main contributors influencing health plants. Additionally, presence pathogen showed a strong negative correlation with phosphorus levels, while was significantly correlated communities. We found network interactions in healthy plant rhizosphere soils more complex compared diseased soils. The had linkages, particularly related occurrence. Within network, family Comamonadaceae, specifically Ramlibacter_tataouinensis , enriched samples pathogen. terms archaea, Halorubrum Halorussus_halophilus (family: Halobacteriaceae), Natronomonas_pharaonis Haloarculaceae) correlations . These findings suggested these archaea may potentially reduce occurrence disease. On other hand, Halostagnicola_larseniia Haloterrigena _sp._BND6 Natrialbaceae) higher relative abundance plants exhibited positive indicating potential contribution pathogen’s Moreover, explored possibility functional gene sharing among correlating pairs within Molecular Ecological Network. analysis 468 entries horizontal transfer (HGT) events, emphasizing significance HGT shaping adaptive traits plant-associated bacteria, relation host colonization pathogenicity. Overall, work key factors, patterns mechanisms underlying populations. offer valuable guidance effectively controlling soil-borne diseases developing sustainable agriculture practices.

Язык: Английский

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Nisin-based therapy: a realistic and eco-friendly biocontrol strategy to contrast Xylella fastidiosa subsp. pauca infections in planta

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Май 15, 2024

The lack of sustainable strategies for combating Xylella fastidiosa ( Xf ) highlights the pressing need novel practical antibacterial tools. In this study, Lactococcus lactis subsp. strain ATCC 11454 L. ), known its production nisin A, was in vitro tested against pauca . Preliminary investigations showed that A involved a strong antagonistic activity exhibited by Thus, efficacy comprehensively assessed through combination and planta experiments. employing viable-quantitative PCR, spot assay, turbidity reduction fluorescence microscopy, transmission electron microscopy demonstrated nisin’s robust bactericidal effect on at minimal lethal concentration 0.6 mg/mL. Moreover, results from microscopies indicated directly rapidly interacts with membranes cells, leading to destruction bacterial cells few minutes. tests, also ability tackle infections within Nicotiana benthamiana plants remained asymptomatic 74 days post inoculation. Furthermore, RPLC-ESI-MS/MS analyses translocated all parts remains intact up 9 days. For first time, study underscores nisin-based strategy as realistic eco-friendly approach be further investigated field.

Язык: Английский

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