Dual column chromatography combined with high-resolution mass spectrometry improves coverage of non-targeted analysis of plant root exudates DOI Creative Commons
Alexandra Bennett, Teresa Mairinger, Çağla Görkem Eroğlu

и другие.

Analytica Chimica Acta, Год журнала: 2024, Номер 1327, С. 343126 - 343126

Опубликована: Авг. 22, 2024

Within the plant kingdom, there is an exceptional amount of chemical diversity that has yet to be annotated. It for this reason non-targeted analysis interest those working in novel natural products. To increase number and compounds observable root exudate extracts, several workflows which differ at three key stages were compared: 1) sample extraction, 2) chromatography, 3) data preprocessing. Plants grown Hoagland's solution two weeks, exudates initially extracted with water, followed by a 24-h regeneration period subsequent extraction using methanol. Utilizing second showed improved results less ion suppression reduced retention time shifting compared first extraction. A single column method, utilizing pentafluorophenyl column, paired high-resolution mass spectrometry ionized correctly identified 34 mock compounds, while dual incorporating porous graphitic carbon retained 43 compounds. In pooled quality control method detected 1,444 While fewer overall (1,050), it revealed larger small polar Three preprocessing methods (targeted, proprietary, open source) successfully 43, 31, 38 confidence level 1, respectively. Enhancing signal strength analytical stability involves removing high ionic nutrient before sampling extracts. Despite intensity loss, enhances compound coverage, particularly metabolites. Open-source software proves viable alternative analysis, even surpassing proprietary peak picking.

Язык: Английский

Jasmonic Acid as a Mediator in Plant Response to Necrotrophic Fungi DOI Creative Commons
Violetta K. Macioszek,

Tomasz Jęcz,

Iwona Ciereszko

и другие.

Cells, Год журнала: 2023, Номер 12(7), С. 1027 - 1027

Опубликована: Март 27, 2023

Jasmonic acid (JA) and its derivatives, all named jasmonates, are the simplest phytohormones which regulate multifarious plant physiological processes including development, growth defense responses to various abiotic biotic stress factors. Moreover, jasmonate plays an important mediator's role during interactions with necrotrophic oomycetes fungi. Over last 20 years of research on physiology genetics JA-dependent pathogens herbivorous insects, beginning from discovery JA co-receptor CORONATINE INSENSITIVE1 (COI1), has speeded up in gathering new knowledge complexity innate immunity signaling. It been observed that biosynthesis accumulation jasmonates induced specifically plants resistant fungi (and also hemibiotrophs) such as mostly investigated model ones, i.e.,

Язык: Английский

Процитировано

42
β-Aminobutyric acid activates SA-signalling systemic acquired resistance in peach fruit by suppressing the circadian clock associated protein1 DOI
Chunhong Li, Yu Xia, Xiang Fei

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140040 - 140040

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1
Melatonin-Induced Stress Enhanced Biomass and Production of High-Value Secondary Cell Products in Submerged Adventitious Root Cultures of Stevia rebaudiana (Bert.) DOI Creative Commons

Bakht Naz,

Aftab Afzal, Hina Ali

и другие.

ACS Omega, Год журнала: 2024, Номер 9(5), С. 5548 - 5562

Опубликована: Янв. 26, 2024

Stress is one of the important factors that directly or indirectly affects plant architecture, biochemical pathways, and growth development. Melatonin (MEL) an stress hormone; however, exogenous addition melatonin to culture media stimulates defense mechanism releases higher quantities secondary metabolites. In this study, submerged adventitious root cultures (SARCs) diabetically

Язык: Английский

Процитировано

8
Advances in functional studies of plant MYC transcription factors DOI
Zewei Li,

Yunshuai Huang,

Zhiwei Shen

и другие.

Theoretical and Applied Genetics, Год журнала: 2024, Номер 137(8)

Опубликована: Авг. 1, 2024

Язык: Английский

Процитировано

7
The Roles of Circadian Clock Genes in Plant Temperature Stress Responses DOI Open Access
Juna Jang, Sora Lee, Jeong‐Il Kim

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(2), С. 918 - 918

Опубликована: Янв. 11, 2024

Plants monitor day length and memorize changes in temperature signals throughout the day, creating circadian rhythms that support timely control of physiological metabolic processes. The

Язык: Английский

Процитировано

6
New insights into light spectral quality inhibits the plasticity elongation of maize mesocotyl and coleoptile during seed germination DOI Creative Commons
Xiaoqiang Zhao, Yining Niu, Zakir Hossain

и другие.

Frontiers in Plant Science, Год журнала: 2023, Номер 14

Опубликована: Март 15, 2023

The plastic elongation of mesocotyl (MES) and coleoptile (COL), which can be repressed by light exposure, plays a vital role in maize seedling emergence establishment under adverse environmental conditions. Understanding the molecular mechanisms light-mediated repression MES COL will allow us to develop new strategies for genetic improvement these two crucial traits maize. A variety, Zheng58, was used monitor transcriptome physiological changes response darkness, as well red, blue, white light. significantly inhibited spectral quality this order: blue > red Physiological analyses revealed that inhibition closely related dynamics phytohormones accumulation lignin deposition tissues. In levels indole-3-acetic acid, trans-zeatin, gibberellin 3, abscisic acid decreased COL; contrast, jasmonic salicylic lignin, phenylalanine ammonia-lyase, peroxidase enzyme activity increased. Transcriptome analysis multiple differentially expressed genes (DEGs) involved circadian rhythm, phytohormone biosynthesis signal transduction, cytoskeleton cell wall organization, biosynthesis, starch sucrose metabolism. These DEGs exhibited synergistic antagonistic interactions, forming complex network regulated elongation. Additionally, gene co-expression 49 hub one 19 modules were associated with plasticity MES, respectively. findings enhance our knowledge light-regulated COL, provide theoretical foundation developing elite varieties improved abiotic stress resistance.

Язык: Английский

Процитировано

14
GIGANTEA Unveiled: Exploring Its Diverse Roles and Mechanisms DOI Open Access
Ling Liu, Yuxin Xie,

Baba Salifu Yahaya

и другие.

Genes, Год журнала: 2024, Номер 15(1), С. 94 - 94

Опубликована: Янв. 13, 2024

GIGANTEA (GI) is a conserved nuclear protein crucial for orchestrating the clock-associated feedback loop in circadian system by integrating light input, modulating gating mechanisms, and regulating clock resetting. It serves as core component which transmits blue signals rhythm resetting overseeing floral initiation. Beyond functions, GI influences various aspects of plant development (chlorophyll accumulation, hypocotyl elongation, stomatal opening, anthocyanin metabolism). has also been implicated to play pivotal role response stresses such freezing, thermomorphogenic stresses, salinity, drought, osmotic stresses. Positioned at hub complex genetic networks, interacts with hormonal signaling pathways like abscisic acid (ABA), gibberellin (GA), salicylic (SA), brassinosteroids (BRs) multiple regulatory levels. This intricate interplay enables balance stress responses, promoting growth flowering, optimize productivity. review delves into multifaceted roles GI, supported molecular evidence, recent insights dynamic between flowering enhance plants’ adaptability environmental challenges.

Язык: Английский

Процитировано

5
Phylogenetic and Expression Analysis of the Sucrose Synthase and Sucrose Phosphate Synthase Gene Family in Potatoes DOI Creative Commons
Jun Hu, Yanfeng Duan,

Jinxue Hu

и другие.

Metabolites, Год журнала: 2024, Номер 14(1), С. 70 - 70

Опубликована: Янв. 20, 2024

Sucrose synthase (SUS) and sucrose phosphate (SPS) are essential in plant metabolism. The potato is an important crop worldwide, but systematic analyses of the StSUS StSPS gene families potatoes still lacking. Ten metabolism-related genes were identified this study. SUSs SPSs could each be split into three subgroups through phylogenetic analysis. StSUSIc was most highly expressed different developmental tissues. Ka/Ks analysis showed that StSUSIb subjected to more-significant homozygous selection pressure. Our cis-acting element promoter sequences four elements: defense- stress-responsive, hormone-responsive, light-responsive, transcription factor elements. expression found regulated by circadian rhythm. In treatments 1% 5% sucrose, glucose, fructose, family enhanced inhibited at high-glucose fructose concentrations. This study six analyzed their structure, conserved motifs, chromosome position, elements, tree, tissue-specific patterns. results will motivate more research biological process underlying metabolism potatoes.

Язык: Английский

Процитировано

5
Metabolome and transcriptome reprogramming underlying tomato drought resistance triggered by a Pseudomonas strain DOI Open Access
Αναστασία Παπαδοπούλου,

Aggeliki Ainalidou,

Ιfigeneia Mellidou

и другие.

Plant Physiology and Biochemistry, Год журнала: 2023, Номер 203, С. 108080 - 108080

Опубликована: Окт. 1, 2023

Язык: Английский

Процитировано

11
Transcriptomic and Phenotypic Analyses Reveal the Molecular Mechanism of Dwarfing in Tetraploid Robinia pseudoacacia L. DOI Open Access
Yue Wu, Qi Guo, Long Cui

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(2), С. 1312 - 1312

Опубликована: Янв. 21, 2024

Polyploid breeding techniques aid in the cultivation of new forestry cultivars, thus expanding suite strategies for improvement arboreal traits and innovation within field forestry. Compared to diploid Robinia pseudoacacia L. (black locust) ‘D26-5①’ (2×), its dwarfed homologous tetraploid ‘D26-5②’ (4×) variety has better application prospects garden vegetation guardrails urban landscape. However, molecular mechanism generation growth this dwarf is still unclear. Here, plant development as well histological differences between autotetraploid were investigated. Levels endogenous hormones at three different developmental stages (20, 40, 70 days) 2× 4× tissue culture plantlets assessed, it was found that brassinosteroid (BR) contents former significantly higher than latter. Transcriptome sequencing data analysis showed differentially expressed genes (DEGs) enriched hormone synthesis signal transduction, sugar starch metabolism, circadian rhythm pathway, which are closely related development. Therefore, these biological pathways may be important regulatory leading dwarfism slow tetraploids. Additionally, utilizing weighted gene coexpression network (WGCNA), we identified crucial (DEGs)—PRR5, CYP450, SPA1—that potentially underlie observed ploidy variation. This study provides a reference black locusts. Collectively, our results metabolite comparative transcriptomics confirm signaling pathway result

Язык: Английский

Процитировано

4