A cinnamyl alcohol dehydrogenase (CAD) like enzyme leads to a branch in the shikonin biosynthetic pathway inArnebia euchroma DOI Open Access
Ruishan Wang, Changzheng Liu, Sheng Wang

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Янв. 9, 2023

Summary Shikonin derivatives are natural naphthoquinone compounds and the main bioactive components produced by several boraginaceous plants, such as Lithospermum erythrorhizon Arnebia euchroma . Phytochemical researches utilizing L. A. cultured cells both indicate existence of a competing route branching out from shikonin biosynthetic pathway toward benzo/hydroquinones. A previous study has shown that branch point is putative alcohol dehydrogenase converting ( Z )-3’’-hydroxygeranylhydroquinone [( )-3’’-OH-GHQ] to an aldehyde intermediate E )-3’’-oxo-GHQ. However, enzyme involved in reaction not characterized at molecular level yet. In this study, we clone candidate gene belonging cinnamyl (CAD) family, AeHGO , through coexpression analysis transcriptome data sets shikonin-proficient shikonin-deficient cell lines biochemical assays, purified protein reversibly oxidizes )-3’’-OH-GHQ produce )-3’’-oxo-GHQ followed reducing )-3’’-OH-GHQ, resulting equilibrium mixture three compounds. Time course kinetic parameters show with about twice efficient which leads predominance mixture. According report, can be converted deoxyshikonofuran, hydroquinone metabolite plants. Considering there competition for accumulation between benzo/hydroquinones cells, supposed play important role metabolic regulation pathway.

Язык: Английский

Insights into the phylogeny and chloroplast genome evolution of Eriocaulon (Eriocaulaceae) DOI Creative Commons
Enze Li, Kangjia Liu, Rongyan Deng

и другие.

BMC Plant Biology, Год журнала: 2023, Номер 23(1)

Опубликована: Янв. 14, 2023

Eriocaulon is a wetland plant genus with important ecological value, and one of the famous taxonomically challenging groups among angiosperms, mainly due to high intraspecific diversity low interspecific variation in morphological characters species within this genus. In study, 22 samples representing 15 from China, were sequenced combined published test phylogenetic resolution using complete chloroplast genome. Furthermore, comparative analyses genomes performed investigate genome evolution Eriocaulon.The nine proved highly similar size, gene content, order. The exhibited typical quadripartite structures lengths 150,222 bp 151,584 bp. Comparative revealed that four mutation hotspot regions (psbK-trnS, trnE-trnT, ndhF-rpl32, ycf1) could serve as effective molecular markers for further identification species. Phylogenetic results supported monophyletic group. identified relationships taxonomic treatment section Heterochiton Leucantherae, was first divergent tree divided into five clades. divergence times indicated all sections diverged later Miocene most extant Quaternary. phylogeny rapid radiation occurred history Eriocaulon.Our study mostly at level China demonstrated power whole sequences. developed can help us better identify understand evolutionary future.

Язык: Английский

Процитировано

37
Maternal Donor and Genetic Variation of Lagerstroemia indica Cultivars DOI Open Access
Chang Guo, Kangjia Liu, Enze Li

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(4), С. 3606 - 3606

Опубликована: Фев. 10, 2023

Lagerstroemia indica L. is a well-known ornamental plant with large pyramidal racemes, long flower duration, and diverse colors cultivars. It has been cultivated for nearly 1600 years essential investigating the germplasm assessing genetic variation to support international cultivar identification breeding programs. In this study, 20 common cultivars from different varietal groups morphologies, as well multiple wild relative species, were analyzed investigate maternal donor of discover relationships among based on plastome nuclear ribosomal DNA (nrDNA) sequences. A total 47 single nucleotide polymorphisms (SNPs) 24 insertion/deletions (indels) identified in cultivars' 25 SNPs nrDNA. Phylogenetic analysis sequences showed that all formed clade species indica, indicating was Population structure PCA analyses supported two clades cultivars, which exhibited significant differences according dataset. The results nrDNA divided into three most had at least backgrounds higher gene flow. Our suggest can be used molecular markers

Язык: Английский

Процитировано

18
Integrative Chinese-Western medicine strategy to overcome docetaxel resistance in prostate cancer DOI
Lin Chen, Yuxin Xu,

Yuanshuo Wang

и другие.

Journal of Ethnopharmacology, Год журнала: 2024, Номер 331, С. 118265 - 118265

Опубликована: Апрель 25, 2024

Язык: Английский

Процитировано

4
Shikonin-loaded PLGA nanoparticles: A promising strategy for psoriasis treatment DOI Creative Commons
Jing Fu, Longtai You,

Daohan Sun

и другие.

Heliyon, Год журнала: 2024, Номер 10(11), С. e31909 - e31909

Опубликована: Май 24, 2024

Psoriasis is an inflammation-based skin illness marked by aggravated proliferation of epidermal cells. Shikonin a natural naphthoquinone obtained from Arnebiae radix. It exerts anti-inflammatory and immunosuppressive effects. However, the poor water solubility low bioavailability shikonin limit its application. In this study, shikosin-loaded PLGA nanoparticle hydrogel was prepared used to deliver drug epidermis psoriasis mice through local administration. The results demonstrated that nanoparticles inhibited HaCaT cell multiplication, increased uptake, induced apoptosis Results Western blotting assays indicated shikosin down-regulated protein expressions p65 p-p65. Furthermore, mitigated decreased concentrations inflammation-inducing cytokines, i.e., IL17A, IL-17F, IL-22, IL-1β, TNF-α. Taken together, these suggest shikonin-PLGA loaded in system possess promising therapeutic potential for psoriasis.

Язык: Английский

Процитировано

3
Shikonin potentiates therapeutic efficacy of oxaliplatin through reactive oxygen species‐mediated intrinsic apoptosis and endoplasmic reticulum stress in oxaliplatin‐resistant colorectal cancer cells DOI
Zhengguang Zhang,

Cunsi Shen,

Fuqiong Zhou

и другие.

Drug Development Research, Год журнала: 2023, Номер 84(3), С. 537 - 550

Опубликована: Фев. 13, 2023

Abstract Oxaliplatin (OXA) has been recognized as a third‐generation platinum‐based chemotherapeutic agent with stellar therapeutic efficacy in managing colorectal cancer (CRC). Nevertheless, resistance to OXA CRC patients hinders its effectiveness. Shikonin (SHI), natural naphthoquinone derived from Arnebia euchroma (Royle) Johnst. , features broad pharmacological profile and minimal toxicities. To assess the synergism of SHI towards OXA‐resistant cells (HCT116 R ), we employed vitro vivo assays. Our experiments provided evidence that SHI, either alone or combination OXA, considerably reduced cell proliferation, triggered apoptosis, induced generation reactive oxygen species (ROS) HCT116 cells. Furthermore, dramatically curbed extent ‐initiated xenograft growth mouse models. Bioinformatics, western blot, ROS assays highlighted mechanisms against may involve induction cellular responses chemical stress, intrinsic well endoplasmic reticulum stress pathways mediated by ROS. Notably, SHI+OXA was partially abrogated an inhibitor N‐acetyl cysteine. findings imply potential boost sensitivity CRC, offering promising benefits for clinical strategies combat resistance.

Язык: Английский

Процитировано

7
Pueraria montana Population Structure and Genetic Diversity Based on Chloroplast Genome Data DOI Creative Commons
Jiahui Sun, Yiheng Wang,

Ping Qiao

и другие.

Plants, Год журнала: 2023, Номер 12(12), С. 2231 - 2231

Опубликована: Июнь 6, 2023

Despite having a generally conserved structure, chloroplast genome data have been helpful for plant population genetics and evolution research. To mine Pueraria montana variation architecture phylogeny, we investigated the of 104 P. accessions from across China. montana's showed high diversity levels, with 1674 variations, including 1118 single nucleotide polymorphisms 556 indels. The intergenic spacers, psbZ-trnS ccsA-ndhD, are two mutation hotspot regions in genome. Phylogenetic analysis based on dataset supported four clades. variations were among within clades, which gene flow levels. Most clades estimated to diverged at 3.82-5.17 million years ago. Moreover, East Asian summer monsoon South may accelerated divergence. Our results show that sequences highly variable can be used as molecular markers assess genetic relationships montana.

Язык: Английский

Процитировано

7
Comparative analysis of jujube and sour jujube gave insight into their difference in genetic diversity and suitable habitat DOI Creative Commons

Lingzhi Shao,

Ping Qiao,

Jingyi Wang

и другие.

Frontiers in Genetics, Год журнала: 2024, Номер 15

Опубликована: Фев. 6, 2024

Jujube ( Ziziphus jujuba var. Mill.) and sour jujube Z. spinosa (Bunge) Hu ex H.F.Chow.) are economically, nutritionally, ecologically significant members of the Rhamnaceae family. Despite their importance, insufficient research on genetics habitats has impeded effective conservation utilization. To address this knowledge gap, we conducted plastome sequencing, integrated distribution data from China, assessed genetic diversity suitable habitat. The plastomes both species exhibited high low diversity. A new-found 23 bp species-specific Indel in petL-petG enabled us to develop a rapid Indel-based identification marker for discrimination. Phylogenetic analysis dating illuminated relationship, showing speciation occurred 6.9 million years ago, period dramatic global temperature fluctuations. Substantial variations climatic conditions were observed, with mean coldest quarter as primary factor influencing distributions (−3.16°C–12.73°C −5.79°C 4.11°C jujube, suitability exceeding 0.6). Consequently, distinct strategies warranted due differences habitats, having broader concentrated Northern China. In conclusion, disparate factors necessitate tailored approaches. Comparing developing primers will further enhance sustainable utilization these valuable species.

Язык: Английский

Процитировано

2
Genomic divergence and demographic history of Quercus aliena populations DOI Creative Commons
Biao Han, Boqiang Tong, Jiliang Zhang

и другие.

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Янв. 9, 2024

Abstract Background Quercus aliena is a major montane tree species of subtropical and temperate forests in China, with important ecological economic value. In order to reveal the species’ population dynamics, genetic diversity, structure, association mountain habitats during evolutionary process, we re-sequenced genomes 72 Q. individuals. Results The whole chloroplast nuclear were used for this study. Phylogenetic analysis using genome dataset supported four clades , while three clades. Sex-biased dispersal had critical role causing discordance between genomes. Population structure showed two groups aliena. effective size sharply declined 1 Mya, coinciding Poyang Glaciation Eastern China. Using genotype–climate analyses, found positive correlation allele frequency variation SNPs temperature, suggesting has capacity adapt changing temperatures. Conclusion Overall, study illustrates divergence, genomic variation, processes behind demographic history .

Язык: Английский

Процитировано

1
The complete chloroplast genome sequence of Lithospermum erythrorhizon: Insights into the phylogenetic relationship among Boraginaceae species and the maternal lineages of purple gromwells DOI Creative Commons
Takahiro Okada, Keiichi Watanabe

Plant Gene, Год журнала: 2024, Номер 37, С. 100447 - 100447

Опубликована: Янв. 21, 2024

Язык: Английский

Процитировано

1
Phylogeny, Genetic Diversity and Population Structure of Fritillaria cirrhosa and Its Relatives Based on Chloroplast Genome Data DOI Open Access
Jiao Huang, Xia Hu, Yong Zhou

и другие.

Genes, Год журнала: 2024, Номер 15(6), С. 730 - 730

Опубликована: Июнь 2, 2024

Fritillaria cirrhosa and its relatives have been utilized in traditional Chinese medicine for many years are under priority protection China. Despite their medicinal protective value, research on phylogeny, genetic diversity, divergence remains limited. Here, we investigate the chloroplast genome variation architecture of 46 samples F. collected from various regions, encompassing majority wild populations across diverse geographical areas. The results indicate abundant variations accessions including 1659 single-nucleotide polymorphisms 440 indels. Six variable markers (psbJ, ndhD, ycf1, ndhG, trnT-trnL, rpl32-trnL) were identified. Phylogenetic network analysis, population structure principal component analysis showed that formed five clades with significant divergence, which related to distribution. regions spanning southern Hengduan Mountains Qinghai–Tibet Plateau exhibited highest levels diversity. may suffered a bottleneck relatively low diversity level. Moreover, barriers discrete patches accelerated divergence. study offers novel perspectives relatives, information can inform conservation utilization strategies future.

Язык: Английский

Процитировано

1