South African Journal of Botany, Год журнала: 2024, Номер 171, С. 454 - 472
Опубликована: Июнь 25, 2024
Язык: Английский
Chemistry & Biodiversity, Год журнала: 2023, Номер 21(2)
Опубликована: Дек. 6, 2023
This study aimed to explore the alkaloid profile of Dendrobium huoshanense and determine potential protective effect against oxidative damage. The crude D. extract (DHAE) was obtained by 70 % ethanol extraction liquid-liquid partition. DHAE contained specific components with abundant 6-hydroxynobiline (58.15 %) trace dendrobine (3.23 in preliminary HPLC fingerprint GC-MS analysis, which distinguished from officinale or nobile. Subsequently, six alkaloids including 6-hydroxynobiline, 2-hydroxy dendrobine, nobilonine, Findlayines D trans-dendrochrysanine were identified purified (namely DHSAE-3, DHSAE-3') via further solid phase coupled UPLC-MS/MS analysis. Meanwhile, pretreatment DHSAE (0.5, 5 μg/mL) increased cell viability 14.0-57.4 compared that H
Язык: Английский
Процитировано
3
Molecules, Год журнала: 2022, Номер 27(22), С. 7688 - 7688
Опубликована: Ноя. 9, 2022
Dehydrocostus lactone (DL) is among the representative ingredients of traditional Chinese medicine (TCM), with excellent anticancer, antibacterial, and anti-inflammatory activities. In this study, an advanced strategy based on ultra-high-performance liquid chromatography-quadrupole-Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) was integrated to comprehensively explore metabolic fate DL in rats. First, prior data collection, all biological samples (plasma, urine, feces) were concentrated purified using solid-phase extraction (SPE) pre-treatment technology. Then, during full-scan (FS) data-dependent acquisition mode, FS-ddMS2 intelligently combined FS-parent ion list (PIL)-dynamic exclusion (DE) means for targeted monitoring deeper capture more low-abundance ions interest. After acquisition, data-mining techniques such as extracted chromatograms (HREICs), multiple defect filters (MMDFs), diagnostic product (DPIs), neutral loss fragments (NLFs) incorporated extensively screen profile metabolites dimensions. As a result, total 71 (parent drug included) positively or tentatively identified. The results suggested that vivo mainly underwent hydration, hydroxylation, dihydrodiolation, sulfonation, methylation, dehydrogenation, dehydration, N-acetylcysteine conjugation, cysteine glutathione glycine taurine etc. With these inferences, we successfully mapped "stepwise radiation" network rats, where several metabolism clusters (DMCs) discovered. conclusion, not only did provide refined inhibiting matrix effects fully screening major-to-trace metabolites, but also give substantial reference mechanism investigation, distribution visualization, safety evaluation DL.
Язык: Английский
Процитировано
3
Foods, Год журнала: 2023, Номер 12(19), С. 3608 - 3608
Опубликована: Сен. 28, 2023
SWATHtoMRM technology was used in this experiment to further identify and trace the sources of Dendrobium devonianum officinale produced same area using TOF MS-MRM. After conversion R package SWATHtoMRM, 191 MRM pairs positive ions 96 negative were obtained. can be separated very well PCA PLS-DA analysis ion pairs; shows that there are obvious differences chemical composition between officinale, which clearly proves pseudotargeted metabolomics method based on for traceability identification research. A total 146 characteristic compounds obtained, with 20 devonianum. The enrichment pathways mainly concentrated lipids atherosclerosis, chagas disease, fluid shear stress proteoglycans cancer, IL-17 signaling pathway, sphingolipid diabetic cardiomyopathy, arginine proline metabolism, etc., among lipid atherosclerosis more enriched, 11 affected expression levels IL-1, TNFα, CD36, IL-1β, etc. These as a reference research variety improvement active substance accumulation officinale.
Язык: Английский
Процитировано
1
South African Journal of Botany, Год журнала: 2024, Номер 171, С. 454 - 472
Опубликована: Июнь 25, 2024
Язык: Английский
Процитировано
0