Plants, Год журнала: 2024, Номер 13(22), С. 3131 - 3131
Опубликована: Ноя. 7, 2024
Somatic embryogenesis (SE) provides alternative methodologies for the propagation of grapevine (
Язык: Английский
Molecular Horticulture, Год журнала: 2025, Номер 5(1)
Опубликована: Янв. 2, 2025
Abstract Grapevine is an important economic fruit tree worldwide, but grape production has been plagued by a vast number of fungal diseases, which affect vigor and the quality yield berries. To seek remedies for such issues, researchers have always committed to conventional biotechnological breeding. In recent years, increasing progress made in elucidating molecular mechanisms grape–pathogenic fungi interactions resistance regulation. Here, we summarize current knowledge on basis grapevine including effector-mediated susceptibility resistance, resistant regulatory networks grapevine, innovative approaches genetic transformation, strategies improve resistance. Understanding exploring accurately regulating diseases.
Язык: Английский
Процитировано
2
Plants, Год журнала: 2024, Номер 13(8), С. 1089 - 1089
Опубликована: Апрель 13, 2024
This paper focuses on the creation of an in vitro collection grapevine hybrids from breeding program Kazakh Scientific Research Institute Fruit Growing and Viticulture investigates presence Plasmopara viticola resistance mediated by Rpv3 Rpv12 loci. We looked at optimization establishment using either shoots taken directly field-grown plants or budwood cuttings forced indoors. further screened for endophyte contamination initiated explants optimized multiplication stage. Finally, loci against P. was studied. The field-sourced were more effective method providing plant sources initiation once all accessions met goal establishment. concentration phytohormones acidity culture medium have a great effect rate quality stock cultures. Out 17 accessions, 16 showed single combined viticola. identified as carrying alleles represent important genetic resources disease programs. These may contribute to new elite cultivars economic interest.
Язык: Английский
Процитировано
7
Deleted Journal, Год журнала: 2025, Номер 2(1)
Опубликована: Фев. 6, 2025
Vincetoxicum spirale (Forssk.) D.Z.Li, a threatened perennial climber with therapeutic values, belongs to the family Apocynaceae (Subfamily: Asclepiadoideae). Constraints related seeds and seedling establishment, coupled wide-spread habitat destruction depleted natural populations of this species. This study presents first report an efficient indirect organogenesis protocol using mature leaf-derived callus V. spirale. Of four types explants evaluated (leaf, node, internode root), leaf were found be most suitable for culture establishment de novo organogenesis. Leaf cultured on Murashige Skoog (MS, 1962) medium containing 2.0 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D) showed highest response (76.7%) induction. Optimum proliferation was achieved MS supplemented 1.0 2,4-D 0.5 Kinetin (Kin), which produced morphogenic, competent, light-green-friable callus. Shoot differentiation observed various concentrations 6-benzylaminopurine (BAP) or Kin alone in combination Indole-3-acetic (IAA) α-naphthalene acetic (NAA). these, consisted BAP, 0.25 Kin, 0.1 NAA, additives (25 each adenine sulphate, L-arginine, citric 50 ascorbic acid) 100 activated charcoal (AC) differentiated number shoots (30.17 ± 1.51) measuring shoot length 6.26 0.82 cm. More than 75% optimally rooted ex vitro after being pulse-treated 300 NAA 5 min. About 70% them successfully survived transplantation polybags. The Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis (SDS-PAGE) protein profiles non-regenerative (NR) regenerative (R) calli compared determine responsible polypeptides their expression levels these two conditions. can employed large-scale regeneration, situ conservation biotechnological applications important climber.
Язык: Английский
Процитировано
0
Plant Cell Tissue and Organ Culture (PCTOC), Год журнала: 2025, Номер 160(3)
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Frontiers in Plant Science, Год журнала: 2023, Номер 14
Опубликована: Дек. 11, 2023
The multifaceted nature of climate change is increasing the urgency to select resilient grapevine varieties, or generate new, fitter cultivars, withstand a multitude new challenging conditions. attainment this goal hindered by limiting pace traditional breeding approaches, which require decades result in selections. On other hand, marker-assisted has proved useful when it comes traits governed one few genes with great effects on phenotype, but its efficacy still restricted for complex controlled many loci. these premises, innovative strategies are emerging could help guide selection, taking advantage genetic diversity within Vitis genus entirety. Multiple germplasm collections also available as source material introgression alleles interest via adapted and pioneering transformation protocols, present themselves promising tools future applications notably recalcitrant species such grapevine. Genome editing intersects both strategies, not only being an alternative obtain focused changes relatively rapid way, supporting fine-tuning genotypes developed methods. A review state art concerning resources possibilities use techniques aid selection presented here support production climate-smart genotypes.
Язык: Английский
Процитировано
10
Plants, Год журнала: 2024, Номер 13(19), С. 2779 - 2779
Опубликована: Окт. 3, 2024
We present data on the ability for organogenesis in 22 genotypes of grapevine and developed a direct protocol cultivar Podarok Magaracha rootstock Kober 5BB. The does not require replacement culture media growth regulators, duration is 11 weeks. cultivation explants occurs modified MS medium with addition 2.0 mg L−1 benzyladenine indole-3-butyric acid (0.15 5BB or 0.05 Magaracha). consists three time periods: (1) culturing 2 weeks dark conditions meristematic bulk tissue, (2) followed by 4 light regeneration, (3) 5 shoot elongation. Based this protocol, Agrobacterium-mediated transformation were an efficiency 2.0% transgenic plants per 100 explants. Two stably transformed lines integration into genome pBin35SGFP plasmid construction, confirmed Southern blotting, obtained.
Язык: Английский
Процитировано
3
In Vitro Cellular & Developmental Biology - Plant, Год журнала: 2024, Номер unknown
Опубликована: Окт. 4, 2024
Язык: Английский
Процитировано
2
Plant Cell Reports, Год журнала: 2024, Номер 43(8)
Опубликована: Июль 24, 2024
Abstract Key message Gene silencing of BcDCL genes improves gray mold disease control in the cultivated strawberry. technology offers new opportunities to develop formulations or pathogen-resistant plants for reducing impacts agricultural systems. Recent studies offered proof concept that symptoms can be reduced by downregulating Dicer-like 1 ( DCL1 ) and 2 DCL2 Botrytis cinerea . In this study, we demonstrate both solutions based on dsRNA topical treatment planta expression targeting BcDCL1 BcDCL2 used strawberry mold, most harmful different fruit crops. 50, 70 100 ng μL −1 naked BcDCL1/2 dsRNA, sprayed Fragaria x ananassa cultivar Romina greenhouse, displayed significant reduction susceptibility, compared negative controls, but a lesser extent than chemical fungicide. Three independent lines were confirmed their stable hairpin gene construct targets Bc-DCL1 sequences (hp-Bc-DCL1/2), production hp construct-derived siRNAs, qRT-PCR Northern blot analyses. vitro vivo detached leaves, fruits from hp-Bc-DCL1/2 showed significantly enhanced tolerance fungal pathogen control. This decreased susceptibility was correlated biomass downregulation B. These results confirm potential RNAi-based products protecting infection, impact pesticides environment health consumers.
Язык: Английский
Процитировано
2
Research Square (Research Square), Год журнала: 2024, Номер unknown
Опубликована: Фев. 6, 2024
Abstract We present data on the ability to organogenesis in 22 genotypes of grapevine and developed a direct protocol for cultivar Podarok Magaracha rootstock Kober 5BB. Protocol does not require replacement culture media growth regulators, duration is 11 weeks. Cultivation explants occurs modified MS medium with addition 2.0 mg l -1 benzyladenine indole-3-butyric acid (0.15 5BB or 0.05 Magaracha). The consists three time periods: (1) culturing 2 weeks dark conditions callogenesis, (2) followed by 4 cultivation light regeneration, (3) 5 shoot elongation. Based this protocol, Agrobacterium -mediated transformation were an efficiency 2.0% transgenic plants per 100 explants. Two stably transformed lines integration into genome pBin35SGFP plasmid construction, confirmed Southern blotting, obtained.
Язык: Английский
Процитировано
1
Опубликована: Авг. 29, 2024
Somatic embryogenesis (SE) provides alternative methodologies for the propagation of grapevine (Vitis spp.) cultivars, conservation its germplasm resources and crop improvement. In this review, current state knowledge regarding SE as applied to these technologies is presented, with a focus on benefits, challenges, limitations method. The paper comprehensive overview different steps involved in process, including callus induction, maintenance embryogenic cultures, production plantlets. Additionally, review explores development high-health plant material through SE, molecular biochemical mechanisms underlying regulation gene expression, hormone signalling pathways, metabolic pathways well use improvement programmes. concludes by highlighting future directions research, new improved protocols, integration other tissue culture techniques, application elite endangered species cultivars unique traits that are valuable breeding programs.
Язык: Английский
Процитировано
1